Cloning of <i>Arabidopsis</i> and barley cDNAs encoding HAK potassium transporters in root and shoot cells

Rubio, Francisco; Santa-Marı́a, Guillermo E.; Rodríguez‐Navarro, Alonso

doi:10.1034/j.1399-3054.2000.100106.x

Cited by 244 publications

(271 citation statements)

References 68 publications

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“…Other such possible routes include nonselective cation channels (NSCCs), such as the cyclic nucleotide gated (CNGC) and/or glutamate receptor (GLR) channels (Lemtiri-Chlieh and Berkowitz 2004;Meyerhoff et al 2005;Wolf et al 2005;Zhao et al 2011; see also Véry et al 1998;Tyerman and Skerrett 1999). It is also possible that members of the KUP/HAK/KT family, generally attributed to primary K + uptake in roots (Gierth and Mäser 2007), might contribute, as these have been shown to be capable of mediating low-affinity Na + fluxes in roots under special circumstances (Santa-María et al 1997;Takahashi et al 2007; see also Mäser et al 2002), and are also expressed in shoots (Kim et al 1998;Rubio et al 2000;Bañuelos et al 2002;Su et al 2002). However, a specific demonstration in guard cell opening and closing has not been made in any study of which we are aware.…”

Section: Sodium As a Nutrientmentioning

confidence: 99%

Sodium as nutrient and toxicant

et al. 2013

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Background Sodium (Na + ) is one of the most intensely researched ions in plant biology and has attained a reputation for its toxic qualities. Following the principle of Theophrastus Bombastus von Hohenheim (Paracelsus), Na + is, however, beneficial to many species at lower levels of supply, and in some, such as certain C4 species, indeed essential. Scope Here, we review the ion's divergent roles as a nutrient and toxicant, focusing on growth responses, membrane transport, stomatal function, and paradigms of ion accumulation and sequestration. We examine connections between the nutritional and toxic roles throughout, and place special emphasis on the relationship of Na + to plant potassium (K + ) relations and homeostasis. Conclusions Our review investigates intriguing connections and disconnections between Na + nutrition and toxicity, and concludes that several leading paradigms in the field, such as on the roles of Na + influx and tissue accumulation or the cytosolic K + /Na + ratio in the development of toxicity, are currently insufficiently substantiated and require a new, critical approach.

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Section: Sodium As a Nutrientmentioning

confidence: 99%

Sodium as nutrient and toxicant

et al. 2013

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“…Another member of the same family, KUP12, was down-regulated in shoots after K 1 resupply (Evalue 0.0079, FDR ,0.004%; see supplemental material). None of the KUP/HAK genes that have been functionally characterized to date seem to be involved in K 1 nutrition although many of them transport K 1 when expressed in heterologous systems (Fu and Luan, 1998;Kim et al, 1998;Rubio et al, 2000), but some of them produce developmentally impaired phenotypes when knocked-out in Arabidopsis (Rigas et al, 2001;Elumalai et al, 2002).…”

Section: Peroxidasesmentioning

confidence: 99%

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

2004

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Full genome microarrays were used to assess transcriptional responses of Arabidopsis seedlings to changing external supply of the essential macronutrient potassium (K+). Rank product statistics and iterative group analysis were employed to identify differentially regulated genes and statistically significant coregulated sets of functionally related genes. The most prominent response was found for genes linked to the phytohormone jasmonic acid (JA). Transcript levels for the JA biosynthetic enzymes lipoxygenase, allene oxide synthase, and allene oxide cyclase were strongly increased during K+ starvation and quickly decreased after K+ resupply. A large number of well-known JA responsive genes showed the same expression profile, including genes involved in storage of amino acids (VSP), glucosinolate production (CYP79), polyamine biosynthesis (ADC2), and defense (PDF1.2). Our findings highlight a novel role of JA in nutrient signaling and stress management through a variety of physiological processes such as nutrient storage, recycling, and reallocation. Other highly significant K+-responsive genes discovered in our study encoded cell wall proteins (e.g. extensins and arabinogalactans) and ion transporters (e.g. the high-affinity K+ transporter HAK5 and the nitrate transporter NRT2.1) as well as proteins with a putative role in Ca2+ signaling (e.g. calmodulins). On the basis of our results, we propose candidate genes involved in K+ perception and signaling as well as a network of molecular processes underlying plant adaptation to K+ deficiency.

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“…In Arabidopsis (Arabidopsis thaliana), the high-affinity K + transporter HAK5 (Rubio et al, 2000;Nieves-Cordones et al, 2010) and the Shaker inward K + channel AKT1 (Lagarde et al, 1996;Hirsch et al, 1998;Spalding et al, 1999;Ivashikina et al, 2001) have been considered two major components that conduct K + uptake in root cells under low-K + (LK) conditions (Gierth et al, 2005;Pyo et al, 2010;Nieves-Cordones et al, 2014). The transcription of HAK5 is induced by K + deficiency (Gierth et al, 2005), which is an important mechanism in plant responses to LK stress .…”

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confidence: 99%

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

et al. 2016

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In Arabidopsis (Arabidopsis thaliana), the Shaker K + channel AKT1 conducts K + uptake in root cells, and its activity is regulated by CBL1/9-CIPK23 complexes as well as by the AtKC1 channel subunit. CIPK23 and AtKC1 are both involved in the AKT1-mediated low-K + (LK) response; however, the relationship between them remains unclear. In this study, we screened suppressors of low-K + sensitive [lks1 (cipk23)] and isolated the suppressor of lks1 (sls1) mutant, which suppressed the leaf chlorosis phenotype of lks1 under LK conditions. Map-based cloning revealed a point mutation in AtKC1 of sls1 that led to an amino acid substitution (G322D) in the S6 region of AtKC1. The G322D substitution generated a gain-of-function mutation, AtKC1 D , that enhanced K + uptake capacity and LK tolerance in Arabidopsis. Structural prediction suggested that glycine-322 is highly conserved in K + channels and may function as the gating hinge of plant Shaker K + channels. Electrophysiological analyses revealed that, compared with wild-type AtKC1, AtKC1 D showed enhanced inhibition of AKT1 activity and strongly reduced K + leakage through AKT1 under LK conditions. In addition, phenotype analysis revealed distinct phenotypes of lks1 and atkc1 mutants in different LK assays, but the lks1 atkc1 double mutant always showed a LK-sensitive phenotype similar to that of akt1. This study revealed a link between CIPK-mediated activation and AtKC1-mediated modification in AKT1 regulation. CIPK23 and AtKC1 exhibit distinct effects; however, they act synergistically and balance K + uptake/leakage to modulate AKT1-mediated LK responses in Arabidopsis.

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Cloning of Arabidopsis and barley cDNAs encoding HAK potassium transporters in root and shoot cells

Cited by 244 publications

References 68 publications

Sodium as nutrient and toxicant

Sodium as nutrient and toxicant

The Potassium-Dependent Transcriptome of Arabidopsis Reveals a Prominent Role of Jasmonic Acid in Nutrient Signaling

AtKC1 and CIPK23 Synergistically Modulate AKT1-Mediated Low-Potassium Stress Responses in Arabidopsis

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