Cloning of Higher Plant [omega]-3 Fatty Acid Desaturases

Yadav, Narendra Singh; Wierzbicki, Anna; Aegerter, M; Caster, C. S.; Pérez-Grau, Lluis; Kinney, Anthony J.; Hitz, William D.; Booth, J. R.; Schweiger, Bruce; Stecca, Kevin L.; Allen, Stephen M.; Blackwell, Meredith; Reiter, Robert S.; Carlson, Thomas J.; Russell, Sandra H.; Feldmann, Kenneth A.; Pierce, James C.; Browse, John

doi:10.1104/pp.103.2.467

Cited by 265 publications

(204 citation statements)

References 29 publications

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“…PCC7002 and Anabaena variabilis [15], and also in those of higher plants such as Arabidopsis thaliana [28], rapeseed [29], soybean [29] and spinach [30], as well as in the o93 acyl-lipid desaturases of Synechocystis sp. PCC6803 [9], A. thaliana [31][32][33], rapeseed [33], soybean [33] and mung bean [34]. However, this histidine residue is not conserved in the/19 and/16 desaturases of Synechocystis sp.…”

Section: Site-directed Mutagenesand Of A12 Desaturasementioning

confidence: 99%

Site‐directed mutagenesis of histidine residues in the Δ12 acyl‐lipid desaturase of Synechocystis

et al. 1995

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In the cyanobacterium Synechocystis sp. PCC 6803, there are four acyl-lipid desaturases that are, respectively, specific to the A6, A9, A12 and to3 positions of fatty acids. The desA gene for the A12 acyl-lipid desaturase was modified by sitedirected mutagenesis, such that four of the histidine residues that are conserved in the four desaturases and one histidine residue that is not conserved were replaced by arginine, and the mutated desA genes were overexpressed in Escherichia coil All of these mutations eliminated the A12 desaturase activity. These results demonstrate that the five histidine residues are essential for the activity of the A12 desaturase, perhaps by providing the ligands for the catalytic Fe center.

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Section: Site-directed Mutagenesand Of A12 Desaturasementioning

confidence: 99%

Site‐directed mutagenesis of histidine residues in the Δ12 acyl‐lipid desaturase of Synechocystis

et al. 1995

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“…Primers generated from base sequences conserved between microsomal w-3 fatty acid desaturases from mung bean (Yamamoto et al, 1992) and rapeseed (Arondel et al, 1992) (upstream primer, 5'-ATTCCNAAGCATTCCTGC-3'; downstream primer, 5'-ACCATCATCATCCAAGTA-3'). (Iba et al, 1993), 67.9% identical to the ER u-3 fatty acid desaturase (Yadav et al, 1993), 34.7% identical to the ER fatty acid desaturase (Okuley et al, 1994) in Arabidopsis. Subcellular Location:…”

mentioning

confidence: 99%

“…By screening a cDNA library with one of the PCR-derived sequences, four positive clones were obtained. Three of these clones encoded a plastid w-3 fatty acid desaturase reported by Iba et al (1993) and Yadav et al (1993). The other clone encoded a novel desaturase.…”

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confidence: 99%

“…Two mutants, fud3 and fudD (or fad7), have been established in Arubidopsis that are deficient in enzyme activity in the ER and the chloroplasts, respectively (Browse and Somerville, 1991). Recently, the FAD3 and the FADD genes have been cloned by T-DNA tagging (Yadav et al, 1993) and positional cloning (Iba et al, 1993), respectively. These results demonstrate that both gene products are closely related to each other in terms of their amino acid sequences (66% identity).…”

mentioning

confidence: 99%

“…clones for the 0-3 fatty acid desaturase in the ER (Yadav et al, 1993) using the other sequence of the PCR product as a probe.…”

mentioning

confidence: 99%

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A New Isozyme of Plastid [omega]-3 Fatty Acid Desaturase in Arabidopsis thaliana

Watahiki

Yamamoto²

1994

Plant Physiol.

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Effects of developmental age, ambient temperature, and dietary alterations on ?12 desaturase activity in the house cricket,Acheta domesticus

Batcabe

Howell

Blomquist

et al. 2000

Arch. Insect Biochem. Physiol.

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Double bond formation in polyunsaturated fatty acids (PUFA) is mediated by desaturase enzymes. Certain insect species have been found to possess a Delta(12) desaturase, previously thought to occur exclusively in plants. We have begun to characterize this enzyme to determine its relatedness to those found in plants and animals. Desaturase activity can be altered significantly by a number of environmental factors in protozoa, cyanobacteria, plants, fish, and rats. We present evidence here that Delta(12) desaturase activity in Acheta domesticus is affected by developmental stage, starvation, dietary alterations, and fluctuations in ambient temperature. Highest activity is observed during the middle of the penultimate instar and 3 to 6 days after adult emergence. Starvation markedly decreases Delta(12) activity, whereas resumption of feeding on fat-free or low fat diets increases activity.

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Cloning of Higher Plant [omega]-3 Fatty Acid Desaturases

Cited by 265 publications

References 29 publications

Site‐directed mutagenesis of histidine residues in the Δ12 acyl‐lipid desaturase of Synechocystis

Site‐directed mutagenesis of histidine residues in the Δ12 acyl‐lipid desaturase of Synechocystis

A New Isozyme of Plastid [omega]-3 Fatty Acid Desaturase in Arabidopsis thaliana

Effects of developmental age, ambient temperature, and dietary alterations on ?12 desaturase activity in the house cricket,Acheta domesticus

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