Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish

Rojas, Diego; Perez-Munizaga, Daniela A.; Centanin, Lázaro; Antonelli, Marcelo; Wappner, Pablo; Allende, Miguel L.; Reyes, Ariel E.

doi:10.1016/j.modgep.2006.08.002

Cited by 85 publications

(64 citation statements)

References 33 publications

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“…The prolyl hydroxylase inhibitor dimethyloxallyl glycine (DMOG; 50mM) produced similar results ( Figure 6A-C). Hif1a is expressed in dorsal aorta of the zebrafish embryo 35 ; MO knockdown of hif1a severely decreased HSC formation and blunted the effect of glucose ( Figure 6D-F). To verify the importance of hif1a in coordinating the biological response in HSC production to fluctuations in environmental glucose levels, we examined genomewide expression changes in pooled cohorts of glucose-treated and control embryos at 36 hpf by microarray and ingenuity pathway analysis.…”

Section: Glucose-induced Ros Activates Hif1a To Mediate Effects On Hsmentioning

confidence: 98%

Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC induction in vivo

Harris

Esain

Frechette

et al. 2013

Blood

103

125

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• Glucose metabolism enhances hematopoietic stem cell formation and function in the vertebrate embryo • Glucose metabolism modulates hif1a activity via mitochondrial generation of reactive oxygen species to impact HSC-relevant gene expressionMany pathways regulating blood formation have been elucidated, yet how each coordinates with embryonic biophysiology to modulate the spatiotemporal production of hematopoietic stem cells (HSCs) is currently unresolved. Here, we report that glucose metabolism impacts the onset and magnitude of HSC induction in vivo. In zebrafish, transient elevations in physiological glucose levels elicited dose-dependent effects on HSC development, including enhanced runx1 expression and hematopoietic cluster formation in the aorta-gonad-mesonephros region; embryonic-to-adult transplantation studies confirmed glucose increased functional HSCs. Glucose uptake was required to mediate the enhancement in HSC development; likewise, metabolic inhibitors diminished nascent HSC production and reversed glucose-mediated effects on HSCs. Increased glucose metabolism preferentially impacted hematopoietic and vascular targets, as determined by gene expression analysis, through mitochondrialderived reactive oxygen species (ROS)-mediated stimulation of hypoxia-inducible factor 1a (hif1a). Epistasis assays demonstrated that hif1a regulates HSC formation in vivo and mediates the dose-dependent effects of glucose metabolism on the timing and magnitude of HSC production. We propose that this fundamental metabolic-sensing mechanism enables the embryo to respond to changes in environmental energy input and adjust hematopoietic output to maintain embryonic growth and ensure viability. (Blood. 2013;121(13):2483-2493

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Section: Glucose-induced Ros Activates Hif1a To Mediate Effects On Hsmentioning

confidence: 98%

Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC induction in vivo

Harris

Esain

Frechette

et al. 2013

Blood

103

125

View full text Add to dashboard Cite

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“…To date, HIF-1α sequences have been reported in several fish species, e.g., Atlantic croaker (Micropogonias undulatus) [51], zebrafish (Danio rerio) [52], Wuchang bream (Megalobrama amblycephala) [53], scale-less carp (Gymnocypris przewalskii) [54], ruff (Gymnocephalus cernuus), flounder (Platichthys flesus), perch (Perca fluviatilis), stickleback (Gasterosteus aculeatus), Russian sturgeon (Acipenser gueldenstaedtii) [55], sea bass (Dicentrarchus labrax) [56], Indian catfish (Clarias batrachus) [57], and channel catfish (Ictalurus punctatus) [58]. Additionally, HIF-2α has been identified in zebrafish [52], Atlantic croaker [51], grass carp (Ctenopharyngodon idella) [59], Indian catfish [57], and …”

Section: Hypoxia-inducible Factorsmentioning

confidence: 99%

The hypoxia signaling pathway and hypoxic adaptation in fishes

Xiao

2015

Sci. China Life Sci.

127

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The hypoxia signaling pathway is an evolutionarily conserved cellular signaling pathway present in animals ranging from Caenorhabditis elegans to mammals. The pathway is crucial for oxygen homeostasis maintenance. Hypoxia-inducible factors (HIF-1α and HIF-2α) are master regulators in the hypoxia signaling pathway. Oxygen concentrations vary a lot in the aquatic environment. To deal with this, fishes have adapted and developed varying strategies for living in hypoxic conditions. Investigations into the strategies and mechanisms of hypoxia adaptation in fishes will allow us to understand fish speciation and breed hypoxia-tolerant fish species/strains. This review summarizes the process of the hypoxia signaling pathway and its regulation, as well as the mechanism of hypoxia adaptation in fishes. Approximately 2.5 billion years ago, photosynthesis led to the accumulation of oxygen to levels that were likely toxic to many obligate anaerobes. However, increased availability of atmospheric O 2 led to the evolution of an extraordinarily efficient system of oxidative phosphorylation. In this system, chemical energy stored in the carbon bonds of organic molecules is transferred to the high-energy phosphate bond in ATP, which is then used to power physicochemical reactions in living cells [1]. Additionally, O 2 serves as the final electron acceptor in oxidative phosphorylation, which is not only required for energy production, but is also the direct substrate of many enzymes. Thus, it is critical for the growth, development, and reproduction of organisms. Consequently, metazoans have evolved complicated systems of cellular metabolism and physiology to maintain oxygen homeostasis and have developed a biochemical response to low oxygen levels [2]. There are a number of oxygen-sensing pathways that promote hypoxia tolerance by activating transcription and inhibiting translation: the energy and nutrient sensor mTOR, the unfolded protein response that activates the endoplasmic stress response, and the nuclear factor (NF)-B transcriptional response [3]. However, hypoxia-inducible factors (HIFs) are recognized as master regulators of the cellular response to hypoxic stress [4,5]. The hypoxia signaling pathway is evolutionarily conserved from Caenorhabditis elegans to human beings and it activates similar or homogenous gene expression, resulting in similar physical and biochemical responses. Compared with the terrestrial environment, oxygen concentrations vary greatly in the aquatic environment [6]. Thus, compared with most birds and mammals, fishes are tolerant of this varying oxygen availability. Natural selection by oxygen concentration has facilitated the evolution of fishes with a range of adaptations to variable oxygen concentration. Even in waters at the same latitude, closely related species or different strains within a species exhibit varied adaptations to oxygen concentration. Additionally, closely related fishes distributed in waters at different latitudes exhibit extensive variation in their tolerance of hypoxia. De...

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“…Unlike HIF-1α in these three teleost, HIF-1α of grass carp was highly expressed in the eyes and kidneys (Law et al 2006). HIF-1α of zebrafish was expressed mainly in the gill, ovary and brain (Rojas et al 2007), and HIF-1α of Atlantic croaker, Micropogonias undulatus (Linnaeus, 1766), was abundantly expressed in the brain Qi et al 206 Antarctic eelpout HIF-1α AAZ52828 Cynoglossus semilaevis.Bootstrap values were generated by testing the tree 10000 times using MEGA 5. and gonads (Rahman and Thomas 2007). The distinctive expression of HIF-1α suggested that this gene might possess different physiological functions in different fish species (Chen et al 2012).…”

Section: Expression Analysis Of Hif-1α Inmentioning

confidence: 99%

“…It has been found that the majority of these strategies are regulated by HIF-1, suggesting the important role it plays in hypoxia adaptation. Recent studies showed that different fish HIF-1α possessed distinctive expression patterns under normoxic or hypoxic conditions (Soitamo et al 2001, Law et al 2006, Rahman and Thomas 2007, Rojas et al 2007, Shen et al 2010). Thus, demonstrates that the regulation pathway of HIF-1 in different fish might be different.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

Qi¹,

Wang²,

Zhang³

et al. 2013

Acta Icth et Piscat

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Cloning of hif-1α and hif-2α and mRNA expression pattern during development in zebrafish

Cited by 85 publications

References 33 publications

Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC induction in vivo

Glucose metabolism impacts the spatiotemporal onset and magnitude of HSC induction in vivo

The hypoxia signaling pathway and hypoxic adaptation in fishes

Molecular Cloning and Expression Analysis of Hypoxia Inducible Factor 1α in Tongue Sole, <I>Cynoglossus Semilaevis</I> (Actinopterygii: Pleuronectiformes: Cynoglossidae), Subjected to Acute Hypoxia

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