Cloning of FSHβ, LHβ, and glycoprotein α subunits from the Russian Sturgeon (Acipenser gueldenstaedtii), β-subunit mRNA expression, gonad development, and steroid levels in immature fish

Hurvitz, Avshalom; Degani, Gad; Goldberg, D.A.; Din, Svetlana Yom; Jackson, Karen; Levavi‐Sivan, Berta

doi:10.1016/j.ygcen.2004.09.019

Cited by 52 publications

(42 citation statements)

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“…Conversely, pituitary and plasma concentrations of LH␤ were higher than those of FSH␤ during ovulation and spermiation. And, the expression of FSH␤ gene in immature (2-to 4-year-old) individuals was also reported in Russian sturgeon (Hurvitz et al, 2005). In comparison with Russian sturgeon, the expression of Chinese sturgeon FSH␤ was not detected in all of 2-and 3-year-old males and females, and even in 4-year-old females.…”

Section: Discussionmentioning

confidence: 85%

“…Similarly, the deduced amino acid sequence of AsLH␤ was also conserved in the 12 cysteines and one N-linked glycosylation sites. Hormone glycosylation had been demonstrated to be important for receptor-mediated activation of adenylate cyclase upstream of G-protein activation (Arey et al, 1997;Hurvitz et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Then, the modified cDNAs were ligated to pMD-18T vector (Promega) and the plasmids were used to transform Escherichia coli DH5␣ super competent cells. The plasmid cDNA library was plated to appropriate density to pick AsFSH␤ and AsLH␤ subunits cDNA were amplified by 3 -and 5 -RACE (rapid amplification of cDNA ends) as described previously (Hurvitz et al, 2005). Briefly, gene-specific primers P1 for the AsFSH␤ subunit and P3 for the AsLH␤ subunit (Table 2) were designed according to FSH␤ sequence (GenBank Accession No.…”

Section: Cloning and Sequencingmentioning

confidence: 99%

“…For Chinese sturgeon, mature males and females need at least 9 and 14 years, respectively. However, for Russian sturgeon (A. gueldenstaedtii), most males mature at the age of 4 years, and females reach first sexual maturity from 6 to 12 years (Hurvitz et al, 2005). In sterlet (A. ruthenus), males mature at the age from 3 to 6 years, and most females get sexual maturation before 8 years (Holcik, 1989).…”

Section: Introductionmentioning

confidence: 99%

“…Quérat et al (2000) cloned two ␤-subunits of GTHs in Siberian sturgeon (A. baeri) and termed them FSH and LH. Hurvitz et al (2005) reported GTH-␣, FSH␤ and Above researches had examined the relationship between gonadotropins and reproduction regulation in other sturgeons, but the relative information was almost unknown in the extremely late sexual maturation Chinese sturgeon. The aim of this study was to clone and characterize Chinese sturgeon GTH-␣, FSH␤ and LH␤, and observe their localization and cellular distribution in pituitaries of mature and immature individuals.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Cao

Zhou

Zhang

et al. 2009

Molecular and Cellular Endocrinology

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Cloning and Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Cao

Zhou

Zhang

et al. 2009

Molecular and Cellular Endocrinology

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Ultrastructural investigations on spermatogenesis and spermatozoan morphology in the endangered Adriatic sturgeon, Acipenser naccarii (Chondrostei, Acipenseriformes)

Grandi

Astolfi

Chicca

et al. 2018

Journal of Morphology

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Spermatogenesis was investigated in the Adriatic sturgeon, Acipenser naccarii, by light and electron microscopy. The testis of the unrestricted type had a germinal compartment composed of lobules containing germ cells and Sertoli cells, and separated by a basal lamina from the interstitial compartment, in which Leydig and myoid cells were detected for the first time in Acipenseridae. Spermatogenesis occurred in spermatocysts produced when Sertoli cells became associated with type A spermatogonia of subsequent generations, which produced a clone of synchronized aligned spermatogonia. In primary spermatocytes at zygo-pachytene stage, the large spherical nucleus contained synaptonemal complexes. The smaller secondary spermatocytes were ovoid with a central round nucleus and scarce cytoplasm. Spermatids were interconnected by cytoplasmic bridges until early spermiogenesis. Chromatin initially condensed as long, twisted, and nonhomogeneous fibers and finally as a compact structure made of thick filaments.Early spermatids showed the flagellum, the primordia of centriole complex and of "implantation fossa," followed by the acrosomal vesicle formed by Golgi complexes and a fibrous body associated to centriole complex. The spermatozoan head had 10 postero-lateral projections and a trapezoidal nucleus, a cylindrical midpiece with six to eight mitochondria, the centriole complex, and a "9 + 2" flagellum with a pair of lateral fins. Three helical endonuclear canals crossed the nucleus from the acrosome base to the implantation fossa; their spiralization and that of chromatin fibers suggest a spiral twisting of the nucleus during spermiogenesis. The Sertoli cells performed phagocytosis of degenerating spermatids and spermatozoa. Significant interindividual differences were detected in most morphological parameters of spermatozoa. Data on spermatogenesis in A. naccarii and morphometric measurements on mature spermatozoa provide information about the reproductive biology of the species useful not only for phylogenetic studies but also for evaluation of sperm quality for artificial reproduction projects and restocking of this and other critically endangered sturgeon species. K E Y W O R D S Acipenseridae, Sertoli cells, spermatozoon, testis, transmission electron microscopy

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Cloning of Russian sturgeon (Acipenser gueldenstaedtii) growth hormone and insulin-like growth factor I and their expression in male and female fish during the first period of growth

Din

Hurvitz

Goldberg

et al. 2008

J Endocrinol Invest

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In this study, the GH and IGF-I of the Russian sturgeon (rs), Acipenser gueldenstaedtii, were cloned and sequenced, and their mRNA gene expression determined. In addition, to improve our understanding of the GH function, the expression of this hormone was assessed in young males and females. Moreover, IGF-I expression was quantified in young males and compared to that in older ones. The nucleotide sequence of the rsGH cDNA was 980 bp long and had an open reading frame of 642 bp, beginning with the first ATG codon at position 39 and ending with the stop codon at position 683. A putative polyadenylation signal, AATAAA, was recognized 42 bp upstream of the poly (A) tail. The position of the signal- peptide cleavage site was predicted to be at position 111, yielding a signal peptide of 24 amino-acids (aa) and a mature peptide of 190 aa. When the rsGH aa sequence was compared with other species, the highest degree of identity was found to be with mammalians (66-70% identity), followed by anguilliformes and amphibia (61%) and other fish (39-47%). The level of rsGH mRNA was discovered to be similar in pituitaries of females and males of 5 age groups (1, 2, 3, 4, and 5- yr-old). In females and males, the levels did not change dramatically during the first 5 yr of growth. The partial nucleotide sequence of the rsIGF-I was 445 bp long and had an open reading frame of 396 bp, beginning with the ATG codon at position 50. The position of the signal-peptide cleavage site was predicted to be at position 187, yielding a signal peptide of 44 aa. The highest level of IGF-I mRNA expression was recorded in the kidney of adult sturgeons. The IGF-I mRNA expression levels in the intestine, pituitary gland, and liver were not significantly different. Low levels of expression were found in the brain, heart, and muscle. In most tissues, there was no significant difference between mRNA levels of one and 5-yr-old fish. In conclusion, based on the GH-sequence analysis, A. gueldenstaedtii is genetically distant from other teleosts. The expression of the GH mRNA was similar in males and females, and its level remained constant during the first 5 yr of growth. While the IGF-I mRNA expression differed amongst various tissues, the level in each tissue was similar in 1 and 5-yr-old fish.

show abstract

Cloning of FSHβ, LHβ, and glycoprotein α subunits from the Russian Sturgeon (Acipenser gueldenstaedtii), β-subunit mRNA expression, gonad development, and steroid levels in immature fish

Cited by 52 publications

References 50 publications

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals

Ultrastructural investigations on spermatogenesis and spermatozoan morphology in the endangered Adriatic sturgeon, Acipenser naccarii (Chondrostei, Acipenseriformes)

Cloning of Russian sturgeon (Acipenser gueldenstaedtii) growth hormone and insulin-like growth factor I and their expression in male and female fish during the first period of growth

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