Cloning of cDNAs encoding senescence-associated genes, ACC synthase and ACC oxidase from stored snow pea pods (Pisum sativum L. var saccharatum) and their expression during pod storage

Pariasca, J.A.T.; Sunaga, Akiko; Miyazaki, Takeshi; Hisaka, Hiroyuki; Sonoda, Masaru; Nakagawa, Hiroki; Sato, Takahide

doi:10.1016/s0925-5214(01)00094-1

Cited by 22 publications

(16 citation statements)

References 36 publications

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“…After the database search, sixteen clones of the forward library and eighteen of the reverse presented similarity with a probable protein related to the senescence in snow pea-pods -ssa13 (Pariasca et al, 2001). This gene is downstream regulated, presents low transcription level in the leaves and immature pods and it does not present homology with known database sequences (Pariasca et al, 2001).…”

Section: Resultsmentioning

confidence: 99%

“…This gene is downstream regulated, presents low transcription level in the leaves and immature pods and it does not present homology with known database sequences (Pariasca et al, 2001). Senescence has been associated to genes that econde pathogenesis related proteins (Lers, 2007), degrading enzymes (Drake et al, 1996) and stress response proteins (Sharabi-Schwager et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

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IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

et al. 2015

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Brazil is the largest citrus producer in the world, being responsible for more than 20% of its production, which is, however still low due to phytosanitary issues such as citrus blight. Citrus blight is an anomaly whose causes still have not yet been determined, therefore there are no efficient control measures to minimize the production losses with the use of resistant varieties being considered the most appropriate method. However, little is known about the genes involved in the defense response of the plants to this anomaly. Considering that many physiological alterations associated with plant stress responses are controlled at a transcriptional level, in this study we sought the identification and characterization of the gene expression products differentially expressed in the response to the citrus blight. Through the suppressive subtractive hybridization technique, expressed cDNA libraries were built using mRNAs isolated from "Cravo" lemon tree roots (Citrus limonia L. Osbeck) under "Pera" orange (Citrus sinensis L. Osbeck) of healthy and sick plants. 129 clones were obtained by subtraction and their sequences were compared in databases. 34 of them linked to proteins associated to stress processes, while the others were similar to sequences of unknown functions or did not present similarity with sequences deposited in the databases. 3 genes were selected and their expressions were studied by RT -qPCR in real-time. Plants with citrus blight presented an increase of the expression level in two of those genes, suggesting that these can be directly involved with this anomaly.Index terms: Differential gene expression, subtractive hybridization, RT-qPCR. RESUMOO Brasil é o maior produtor de citros do mundo, sendo responsável por mais de 20% de sua produção. No entanto, a produção ainda é baixa, em decorrência de problemas fitossanitários, como o Declínio do Citros que é uma anomalia cuja causa ainda não foi determinada e, consequentemente, não existem medidas de controle para minimizar as perdas na produção. O uso de variedades resistentes é considerado como a medida de controle mais adequada. Contudo, pouco se conhece sobre os genes envolvidos na resposta de defesa das plantas a essa anomalia. Considerando que muitas alterações fisiológicas associadas com respostas a estresses em plantas são controladas em nível transcripcional, neste estudo objetivou-se a identificação e caracterização dos produtos de expressão gênica diferencialmente expressos na resposta ao Declínio dos Citros. Por meio da técnica de hibridação subtrativa supressiva, bibliotecas de cDNAs expressos foram construídas utilizando mRNAs isolados de raízes de limoeiro "Cravo" (Citrus limonia L. Osbeck) sob laranja "Pera" (Citrus sinensis L. Osbeck) de plantas sadias e doentes. Cento e vinte e nove clones foram obtidos por subtração e suas sequências foram comparadas em bancos de dados. Trinta e quatro delas relacionaram-se a proteínas associadas a processos de estresses, enquanto as outras foram similares a sequências de funções desconheci...

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

et al. 2015

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“…The utrophin homolog is only expressed in the F and W groups and could be involved in contractions associated with secretion. The senescence-associated protein was identified in aged snow peas and has no known function (Pariasca et al, 2001), but it seems to be expressed significantly more in the U group.…”

Section: Discussionmentioning

confidence: 99%

Gene expression in male tick salivary glands is affected by feeding in the presence of females

Anyomi

Bior

Essenberg

et al. 2006

Arch Insect Biochem Physiol

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In this study, we have compared gene expression in the salivary glands of male Dermacentor andersoni ticks fed in the presence or absence of females to that in unfed males. We have established that the patterns of expression are different using arbitrarily primed polymerase chain reaction following reverse transcription of RNA (RAP-PCR) and differential display. Our results indicate a significant difference in salivary gland gene expression between fed and unfed males and also between males fed in the presence of females versus males fed in the absence of females. This difference in expression was partially confirmed by hybridization of probes made from mRNA from the three groups of ticks against the cloned gene fragments. Gene fragments for septin, utrophin, eukaryotic chain release factor 3, and elongation factor 2 were identified in this study, though the majority of fragments were not similar to anything in the databases.

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“…After confirmation of ripeness by measuring ethylene production, total RNA from fresh tissue (mesocarp, placenta, and seed) was extracted using the guanidine thiocyanate method, described previously by Pariasca et al (2001) with modifications. The extraction buffer was composed of 4.2 mM guanidine thiocyanate and 25 mM trisodium citrate dihydrate and added to the tissue in a ratios: 1:2.5 for mesocarp and placental tissue, and 1:5 for seed tissue.…”

Section: Total Rna Isolationmentioning

confidence: 99%

Effect of p-CPA-parthenocarpic setting on the delayed ripening of netted-melon fruits

et al. 2005

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Application of plant growth regulators may induce parthenocarpic fruit setting, avoiding problems due to poor pollination. Their influence on the ripening of netted-melon fruit (Cucumis melo L.), however, is not yet well understood. The ripening behavior of parthenocarpic melon fruit (treated with 50 ppm 4-chlorophenoxy acetic acid (p-CPA) and 350 ppm gibberellic acid (GA 3 ) at anthesis) was compared with that of hand-pollinated fruit. The ethylene production in parthenocarpic fruit peaked up about 10 days behind that of pollinated fruit but was greater. The delay was accompanied by a retardation in fruit softening, thereby leading to an extended edible period. The expression of ethylene synthesis-related enzymes: ACC synthase (ACS) and ACC oxidase (ACO) genes at the commencement of the ripening process was also evaluated. Northern blot and RT-PCR analyses revealed a higher level in the mRNA transcript abundance of ACS genes (auxin-and wound-inducible) but lower abundance of the ripening-related gene ACO in parthenocarpic fruit compared to that of pollinated fruit. Western blot analysis further confirmed the lower level of ACO polypeptide in parthenocarpic fruit. The retarded ripening of parthenocarpic melon fruit resulted from the delayed production of ethylene, which is likely a consequence of the low abundance of ACO. A possible inhibitory effect of auxin on ACO transcript as a result of p-CPA application is proposed.

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Cloning of cDNAs encoding senescence-associated genes, ACC synthase and ACC oxidase from stored snow pea pods (Pisum sativum L. var saccharatum) and their expression during pod storage

Cited by 22 publications

References 36 publications

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

IDENTIFICATION AND QUANTIFICATION OF DIFFERENTIALLY EXPRESSED GENES ASSOCIATED WITH CITRUS BLIGHT (Citrus spp.)

Gene expression in male tick salivary glands is affected by feeding in the presence of females

Effect of p-CPA-parthenocarpic setting on the delayed ripening of netted-melon fruits

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