Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius

Holmes, David; Drocourt, Daniel; Tiraby, Gérard; Cundliffe, Eric

doi:10.1016/0378-1119(91)90532-g

Cited by 35 publications

(26 citation statements)

References 11 publications

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“…The kamA of S. tenjimariensis, kamB of S. tenebrarius NCIB 11028 and kamC of another sporaricin-producing Sac. hirsuta strain, CL102, have been reported to have DNA similarity (Skeggs et al, 1987;Cundliffe, 1989;Holmes et al, 1991). The fmrT-type resistance genes obtained in this study gave a similar resistance profile to that of the karn type resistance genes.…”

Section: Discussionsupporting

confidence: 64%

“…During the preparation of this report, the isolation of kamC (see above for designation of kam) of another sporaricin producer, Sac. hirsuta CL102, was reported by Holmes et al (1991). However, the physical map of our clone was quite different from their kamCcontaining DNA fragment ; the taxonomic relationship of the two strains is unclear.…”

Section: (A) Although Skeggs Et Almentioning

confidence: 57%

“…The resistance pattern of the fmrT-type was similar to the kam genes isolated from S. tenjimariensis (kamA) (Skeggs et al, 1985), a nebramycin producer S. tenebrarius NCIB 11028 (kamB) (Skeggs et al, 1987) and a sporaricin producer Sac. hirsuta CL102 (kamC) (Holmes et al, 1991), in spite of the discrepancy (Fig. 1 a) and the 3.2 kb PvuII fragment in pFMR02 (Fig.…”

Section: Resistance Projiles Of Fmr Genesmentioning

confidence: 87%

“…1 c ) ; however, it differed in size from the 2-6 kb BamHI fragment containing kamC in another sporaricin producer, Sac. hirsuta CL 102 (Holmes et al, 1991). Sac.…”

Section: Distribution Of Dna Fragments Homologous To Fmrt and Fmr0mentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Two Different Types of Resistance Genes Among Producers of Fortimicin-Group Antibiotics

Ohta

Dairi²,

Hasegawa³

1993

Journal of General Microbiology

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Section: Discussionsupporting

confidence: 64%

Section: (A) Although Skeggs Et Almentioning

confidence: 57%

Section: Resistance Projiles Of Fmr Genesmentioning

confidence: 87%

“…1 c ) ; however, it differed in size from the 2-6 kb BamHI fragment containing kamC in another sporaricin producer, Sac. hirsuta CL 102 (Holmes et al, 1991). Sac.…”

Section: Distribution Of Dna Fragments Homologous To Fmrt and Fmr0mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Two Different Types of Resistance Genes Among Producers of Fortimicin-Group Antibiotics

Ohta

Dairi²,

Hasegawa³

1993

Journal of General Microbiology

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“…Until recently, the only target sites that have been experimentally determined were G1405 for KgmB from Streptoalloteichus tenebrarius (62), and A1408 for KamA and KamC from Streptomyces tenjimariensis and Saccharopolyspora hirsute, respectively (22,62). The site of action for other MTases has been usually inferred indirectly by their inability to further methylate ribosome subunits already protected by one of these enzymes, just as we have shown it for Sgm MTase to be G1405 (77).…”

Section: From Fundamental Research To Applicationmentioning

confidence: 96%

rRNA Methyltransferases and their Role in Resistance to Antibiotics

Morić¹,

Savic²,

Ilić-Tomič³

et al. 2010

Journal of Medical Biochemistry

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MTaze metiluju nukleinske kiseline (DNK, RNK) i proteine, moduli{u}i tako njihovu aktivnost, funkciju i strukturnu organizaciju. Metilacija G1405 ili A1408 baza u 16S rRNK mikroorganizama koji proizvode aminoglikozide obezbe |u -je rezistenciju na sopstvene toksi~ne pro izvode. Ovaj mehanizam rezistencije je donedavno bio opi san samo kod proizvo|a~a antibiotika. Od 2003. godine i kod patogenih bakterija bele`i se neprestan porast rezi sten cije na aminoglikozide putem ovog mehanizma, {to predstavlja veliku pretnju efikasnoj upotrebi aminoglikozida u klini~koj praksi. Jedno od mogu}ih re{enja problema le`i u razvoju novih jedinjenja koja bi efikasno delovala na nova mesta u okviru ribozoma. Drugi pristup re{avanju ovog problema uklju~uje razvoj inhibitora MTaza odgovornih za rezisten ciju, sa idejom da se onemogu}i modifikacija bakte rijske rRNK i na taj na~in vrati terapeutska efikasnost postoje}im aminogliko zidima. Fundamentalna istra`ivanja vezana za proteinsku ekspresiju, potpuno razumevanje mehanizma rezistencije kao i razre{enje tercijarne strukture proteina su neophodan preduslov za primenu inhibitora 16S rRNK MTaza u medicini.

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Aminoglycosides and Sugar Components in Other Secondary Metabolites

Piepersberg¹,

Distler²

1997

Biotechnology

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Cloning of an aminoglycoside-resistance-encoding gene, kamC, from Saccharopolyspora hirsuta: comparison with kamB from Streptomyces tenebrarius

Cited by 35 publications

References 11 publications

Characterization of Two Different Types of Resistance Genes Among Producers of Fortimicin-Group Antibiotics

Characterization of Two Different Types of Resistance Genes Among Producers of Fortimicin-Group Antibiotics

rRNA Methyltransferases and their Role in Resistance to Antibiotics

Aminoglycosides and Sugar Components in Other Secondary Metabolites

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