Cloning of a yolk protein gene family from Caenorhabditis elegans

Blumenthal, Thomas; Squire, Michael D.; Kirtland, Sarah; Cane, Jerome; Donegan, Mary; Strouboulis, John; Sharrock, William J.

doi:10.1016/0022-2836(84)90361-9

Cited by 98 publications

(54 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Vitellogenin gene expression is restricted in three ways: by tissue, by sex, and by stage: the mRNA is present in abundance, but only in the adult hermaphrodite intestine (4). We hypothesize that the sequences we have identified are cis-acting elements involved in this developmental regulation.…”

Section: Regulation Of Viteloeni Gene Transcriptionmentioning

confidence: 80%

“…The vitellogenins, precursors of the yolk proteins, are encoded by a small family of genes whose expression is limited by stage, sex, and tissue. They are synthesized in the adult hermaphrodite intestine, cotranslationally secreted into the body cavity, and subsequently taken up by the gonad (3,4). The vitellogenin gene family is composed of six genes, each about 5.1 kb in length including a few short introns (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…Although transcription of the vitellogenin genes is apparently restricted to the 32 cells of the adult hermaphrodite intestine, the mRNAs accumulate to a level sufficient to make them among the most abundant mRNAs in a mixed population of larval and adult worms (4). We cing overlapping fragments cloned from restriction sites discovered by the previous sequencing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

TheC. elegansvitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes

et al. 1985

Self Cite

View full text Add to dashboard Cite

The nematode Caenorhabditis elegans contains a small family of vitellogenin genes which is expressed abundantly, but only in the intestine of the adult hermaphrodite worm. In order to identify possible regulatory elements, we have sequenced the DNA surrounding the 5' ends of five of the six genes. Contained within regions which have largely diverged from one another, two different heptameric sequences are found repeated within the first 200 bp upstream of each of the genes. The first sequence, TGTCAAT, is present as a perfect heptamer at least once upstream of each gene. It is repeated in both orientations four to six times in each 5' flanking region, allowing a one-base mismatch. The second sequence, CTGATAA, is also present as a perfect heptamer in a restricted region upstream of each gene. These two sequence elements may be involved in regulation of the vitellogenin genes. Remarkably, the CTGATAA sequence is present in a similar location in the promoter regions of vertebrate vitellogenin genes. In fact, our data reveal a surprising degree of similarity between the nematode and vertebrate vitellogenins.

show abstract

Section: Regulation Of Viteloeni Gene Transcriptionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TheC. elegansvitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes

et al. 1985

Self Cite

View full text Add to dashboard Cite

show abstract

“…4,6). Often, multiple Vtg genes are present and expressed in a species (4,(22)(23)(24)(25). MTP is present in vertebrates and invertebrates and has been shown to enhance the biosynthesis of other LLTPs, as demonstrated for apolipoproteins and Vtg (26)(27)(28).…”

mentioning

confidence: 99%

Molecular diversity and evolution of the large lipid transfer protein superfamily

Smolenaars

Madsen

Rodenburg

et al. 2007

Journal of Lipid Research

158

160

View full text Add to dashboard Cite

Circulatory lipid transport in animals is mediated to a substantial extent by members of the large lipid transfer (LLT) protein (LLTP) superfamily. These proteins, including apolipoprotein B (apoB), bind lipids and constitute the structural basis for the assembly of lipoproteins. The current analyses of sequence data indicate that LLTPs are unique to animals and that these lipid binding proteins evolved in the earliest multicellular animals. In addition, two novel LLTPs were recognized in insects. Structural and phylogenetic analyses reveal three major families of LLTPs: the apoB-like LLTPs, the vitellogenin-like LLTPs, and the microsomal triglyceride transfer protein (MTP)-like LLTPs, or MTPs. The latter are ubiquitous, whereas the two other families are distributed differentially between animal groups. Besides similarities, remarkable variations are also found among LLTPs in their major lipid-binding sites (i.e., the LLT module as well as the predicted clusters of amphipathic secondary structure): variations such as protein modification and number, size, or occurrence of the clusters. Strikingly, comparative research has also highlighted a multitude of functions for LLTPs in addition to circulatory lipid transport. The integration of LLTP structure, function, and evolution reveals multiple adaptations, which have come about in part upon neofunctionalization of duplicated genes. Moreover, the change, exchange, and expansion of functions illustrate the opportune application of lipid-binding proteins in nature.Accordingly, comparative research exposes the structural and functional adaptations in animal lipid carriers and brings up novel possibilities for the manipulation of lipid transport.

show abstract

“…YP170A, YP170B, YP115 e YP88 (BLUMENTHAL et al,1984). Esses polipeptídios são codificados em seis genes, dos quais cinco estão no cromossomo IV (vit-1 a vit-5) e um no cromossomo X (vit-6) (BLUMENTHAL; HEINE, 1986).…”

Section: Sobrevivência a Anidrobioseunclassified

Caracterização preliminar dos hábitos extremófilos, vitelinas e proteases de Panagrolaimus sp. CEW2, um nematoide de vida livre isolado da Região Amazônica.

Coelho¹

View full text Add to dashboard Cite

Cloning of a yolk protein gene family from Caenorhabditis elegans

Cited by 98 publications

References 30 publications

TheC. elegansvitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes

TheC. elegansvitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes

Molecular diversity and evolution of the large lipid transfer protein superfamily

Caracterização preliminar dos hábitos extremófilos, vitelinas e proteases de Panagrolaimus sp. CEW2, um nematoide de vida livre isolado da Região Amazônica.

Contact Info

Product

Resources

About