Cloning of a New Gene/s in Chromosome 17p3.2-p13.1 that Control Apoptosis

Abstract

Public reporting burden for this collection of information is estimated to average 1 tx)ur per response, including tfie time for reviewing instructions, searctiing existing data sources, gathering and maintaining tfie data needed, and completing and reviewing tfiis collection of infomiation. Send comments regarding ttiis burden estimate or any other aspect of this collection of infomiation, including suggestions for reducing this burden to Washington Headquarters Services, Directorate for Infomtation Operations and Reports, 1215 Jefferson Davis Highway. Suite 1204, Arlington. VA 22202-4302, and to the Office of Management and Budget, Jose Russo, M.D. FUNDING NUMBERSDAMD17-02-1-0384 PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES)Fox Chase Cancer Center Philadelphia, Pennsylvania 19111 Abstract (Maximum 200 Words) {abstract should contain rto oroDrietarv or confidential information)Loss of genetic material (LOH) in the chromosome 17 pl3.2 at the microsateUite marker D17S796 has been identified in atypical ductal hyperplasia and in situ ductal carcinoma of the breast. Our results shown LOH at the same region in MCF-IOF cells treated with the chemical carcinogen benz(a)pyrene (BP). Moreover, microcell-mediated transfer of an intact copy of human chromosome 17 inhibited the tumorigenicity of BPIE and PCR-SSCP analyzes showed a restoration of the lost material in BPlE-17-neo. These experiments suggested the presence of a tumor suppressor gene in 17pl3.2 near the marker D17S796. We have been able to clone a fragment of the genes that could represent the last exon of a bigger peptide. The presence of a 3' splicing site in the putative introns and the ATTAAA region at the 3'end support this idea. The predicted amino acid sequence does not share significant homology with any known protein supporting the idea that this could be a novel protein. Further experiments will be done in order to clone the full-length cDNA and to study the regulation of the expression of this novel gene.