Cloning of a Human cDNA for CTP-Phosphoethanolamine Cytidylyltransferase by Complementation in Vivo of a Yeast Mutant

Nakashima, Asae; Hosaka, K.; Nikawa, Jùn‐ichi

doi:10.1074/jbc.272.14.9567

Cited by 55 publications

(47 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ECT genes have been identified in several eukaryotic species: yeast [85], human [86], rat [87] and the algae C. reinhardtii [88].…”

Section: Choline and Ethanolamine Cytidylyltransferases (Ccts And Ects)mentioning

confidence: 99%

“…Disruption of the D. melanogaster CCT1 gene results also in a reproductive phenotype [91] attributed to aberrant membrane trafficking of ovarian growth and differentiation factor receptors [92]. S. cerevisiae CCT and ECT mutants are not essential for viability [80,86] indicative of the dominant role of the CDP-DAG route to phospholipid biosynthesis in this organism [93]. However, only CCT mutants suppressed the lethal sec14 phenotype [93].…”

Section: Choline and Ethanolamine Cytidylyltransferases (Ccts And Ects)mentioning

confidence: 99%

See 1 more Smart Citation

Comparative genomics and evolution of eukaryotic phospholipid biosynthesis

Lykidis

2007

Progress in Lipid Research

View full text Add to dashboard Cite

Phospholipid biosynthetic enzymes produce diverse molecular structures and are often present in multiple forms encoded by different genes. This work utilizes comparative genomics and phylogenetics for exploring the distribution, structure and evolution of phospholipid biosynthetic genes and pathways in 26 eukaryotic genomes. Although the basic structure of the pathways was formed early in eukaryotic evolution, the emerging picture indicates that individual enzyme families followed unique evolutionary courses. For example, choline and ethanolamine kinases and cytidylyltransferases emerged in ancestral eukaryotes, whereas, multiple forms of the corresponding phosphatidyltransferases evolved mainly in a lineage specific manner.Furthermore, several unicellular eukaryotes maintain bacterial-type enzymes and reactions for the synthesis of phosphatidylglycerol and cardiolipin. Also, base-exchange phosphatidylserine synthases are widespread and ancestral enzymes. The multiplicity of phospholipid biosynthetic enzymes has been largely generated by gene expansion in a lineage specific manner. Thus, these observations suggest that phospholipid biosynthesis has been an actively evolving system. 2Finally, comparative genomic analysis indicates the existence of novel phosphatidyltransferases and provides a candidate for the uncharacterized eukaryotic phosphatidylglycerol phosphate phosphatase.

show abstract

“…ECT genes have been identified in several eukaryotic species: yeast [85], human [86], rat [87] and the algae C. reinhardtii [88].…”

Section: Choline and Ethanolamine Cytidylyltransferases (Ccts And Ects)mentioning

confidence: 99%

Section: Choline and Ethanolamine Cytidylyltransferases (Ccts And Ects)mentioning

confidence: 99%

Comparative genomics and evolution of eukaryotic phospholipid biosynthesis

Lykidis

2007

Progress in Lipid Research

View full text Add to dashboard Cite

show abstract

“…Ethanolaminephosphate is then converted to CDP-ethanolamine by the ethanolaminephosphate cytidylyltransferase (Ect1p/Muq1p). 170,184 In an ect1/muq1 deletion strain, conversion of cholinephosphate to CDP-choline appears to be unaffected. Finally, PtdEtn is formed in the endoplasmic reticulum by CDP-ethanolamine:1,2-diacylglycerol ethanolaminephosphotransferase (Ept1p).…”

Section: Phospholipid Synthesismentioning

confidence: 99%

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Daum

Lees

Bard

et al. 1998

Yeast

579

380

View full text Add to dashboard Cite

The yeast Saccharomyces cerevisiae is a powerful experimental system to study biochemical, cell biological and molecular biological aspects of lipid synthesis. Most but not all genes encoding enzymes involved in fatty acid, phospholipid, sterol or sphingolipid biosynthesis of this unicellular eukaryote have been cloned, and many gene products have been functionally characterized. Less information is available about genes and gene products governing the transport of lipids between organelles and within membranes, turnover and degradation of complex lipids, regulation of lipid biosynthesis, and linkage of lipid metabolism to other cellular processes. Here we summarize current knowledge about lipid biosynthetic pathways in S. cerevisiae and describe the characteristic features of the gene products involved. We focus on recent discoveries in these fields and address questions on the regulation of lipid synthesis, subcellular localization of lipid biosynthetic steps, cross‐talk between organelles during lipid synthesis and subcellular distribution of lipids. Finally, we discuss distinct functions of certain key lipids and their possible roles in cellular processes. © 1998 John Wiley & Sons, Ltd.

show abstract

“…In contrast to CTP:phosphocholine cytidylyltransferase, which is encoded by two genes and exists in four isoforms in the mouse (130, 131), the cytidylyltransferase of the CDP-ethanolamine pathway is encoded by only a single gene that has extensive regions of homology to the corresponding enzyme of the CDP-choline pathway. One difference between the cytidylyltransferases that participate in phosphatidylcholine and PE synthesis is that CTP:phosphoethanolamine cytidylyltransferase contains two copies of the putative catalytic domain, whereas CTP:phosphocholine cytidylyltransferase contains only a single copy of this motif (128). Moreover, unlike CTP: phosphocholine cytidylyltransferase, the activity of CTP: phosphoethanolamine cytidylyltransferase does not reside in the nucleus and is not regulated by reversible translocation between a soluble form and a membraneassociated form (reviewed in Ref.…”

Section: The Cdp-ethanolamine Pathwaymentioning

confidence: 99%

Thematic Review Series: Glycerolipids. Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids

Vance

2008

Journal of Lipid Research

415

355

View full text Add to dashboard Cite

Phosphatidylserine(PS)andphosphatidylethanolamine (PE) are two aminophospholipids whose metabolism is interrelated. Both phospholipids are components of mammalian cell membranes and play important roles in biological processes such as apoptosis and cell signaling. PS is synthesized in mammalian cells by base-exchange reactions in which polar head groups of preexisting phospholipids are replaced by serine. PS synthase activity resides primarily on mitochondria-associated membranes and is encoded by two distinct genes. Studies in mice in which each gene has been individually disrupted are beginning to elucidate the importance of these two synthases for biological functions in intact animals. PE is made in mammalian cells by two completely independent major pathways. In one pathway, PS is converted into PE by the mitochondrial enzyme PS decarboxylase. In addition, PE is made via the CDP-ethanolamine pathway, in which the final reaction occurs on the endoplasmic reticulum and nuclear envelope. The relative importance of these two pathways of PE synthesis has been investigated in knockout mice. Elimination of either pathway is embryonically lethal, despite the normal activity of the other pathway. PE can also be generated from a base-exchange reaction and by the acylation of lyso-PE. Cellular levels of PS and PE are tightly regulated by the implementation of multiple compensatory mechanisms.-Vance, J. E. Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids.

show abstract

Cloning of a Human cDNA for CTP-Phosphoethanolamine Cytidylyltransferase by Complementation in Vivo of a Yeast Mutant

Cited by 55 publications

References 34 publications

Comparative genomics and evolution of eukaryotic phospholipid biosynthesis

Comparative genomics and evolution of eukaryotic phospholipid biosynthesis

Biochemistry, cell biology and molecular biology of lipids ofSaccharomyces cerevisiae

Thematic Review Series: Glycerolipids. Phosphatidylserine and phosphatidylethanolamine in mammalian cells: two metabolically related aminophospholipids

Contact Info

Product

Resources

About