Cloning of a cDNA encoding a plasma membrane-associated, uronide binding phosphoprotein with physical properties similar to viral movement proteins.

Abstract

Oligogalacturonides are structural and regulatory homopolymers from the extracellular pectic matrix of plants. In vitro micromolar concentrations of oligogalacturonates and polygalacturonates were shown previously to stimulate the phosphorylation of a small plasma membrane-associated protein in potato. lmmunologically cross-reactive proteins were detected in plasma membrane-enriched fractions from all angiosperm subclasses in the Cronquist system. Polygalacturonate-enhanced phosphorylation of the protein was observed in four of the six dicotyledon subclasses but not in any of the five monocotyledon subclasses. A cDNA for the protein was cloned from potato. The deduced protein is extremely hydrophilic and has a proline-rich N terminus. The C-terminal half of the protein was predicted to be a coiled coil, suggesting that the protein interacts with other macromolecules. The recombinant protein was found to bind both simple and complex galacturonides. The behavior of the protein suggests severa1 parallels with vira1 proteins involved in intercellular communication.

Genome-Wide Identification of Nodule-Specific Transcripts in the Model Legume Medicago truncatula

“…These include TCs that encode proteins similar to: (a) purine permease, a high-affinity transporter for adenine, cytosine, and purine derivatives (Gillissen et al, 2000); (b) plantacyanin, a plant-specific blue copper protein, apparently involved in the defense response (Nersissian et al, 1998); (c) a homolog of an Arabidopsis embryospecific protein, whose cellular function is yet to be understood; (d) B12D protein, a protein known to be accumulated in plants during embryo development, seed maturation, and leaf senescence (Aalen et al, 1994;Huang et al, 2001); (e) remorin, a membrane phosphoprotein, suggested to be involved in intercellular communications (Reymond et al, 1996); (f) 2-on-2 hemoglobin, the higher plant homolog of the "truncated" hemoglobins found in bacteria, protozoa, and algae, which possess unique biochemical properties that are likely distinct from those of other plant hemoglobins (Watts et al, 2001); and (g) calmodulinlike proteins.…”

Proteome Analysis. Novel Proteins Identified at the Peribacteroid Membrane from Lotus japonicus Root Nodules

“…Remorin is a small hydrophilic protein tightly associated to the PM. It binds uronides and is thought to be a plasmodesmataassociated protein involved in cell-to-cell signaling or transport (Reymond et al, 1996). Interestingly, the enzyme isocitrate lyase normally located in glyoxysomes was also found as a component of complex I.…”

Grain setting defect1, Encoding a Remorin Protein, Affects the Grain Setting in Rice through Regulating Plasmodesmatal Conductance

“…Overexpression of LjSYMREM1, the ortholog of MtSYMREM1 in Lotus japonicus, resulted in increased root nodulation (Lefebvre et al, 2010;Tóth et al, 2012). Although a potential association between remorins and PD permeability has been proposed (Raffaele et al, 2009), the diversity observed across remorins, plus the fact that remorin mutants generated through different approaches fail to show obvious phenotypes (Reymond et al, 1996;Bariola et al, 2004), have made it challenging to characterize the function of remorins in cell-to-cell transport.…”