Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase α-subunit

Macias, M. T.; Palmero, Ignacio; Sastre, Leandro

doi:10.1016/0378-1119(91)90151-z

Cited by 34 publications

(32 citation statements)

References 38 publications

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“…In the present immunocytochemical study, the mouse monoclonal IgGα 5 appears to recognizes its epitope in the branchial cavity of the developing freshwater crayfish, Astacus leptodactylus. This high antibody specificity may be attributable to the conservation of the α-subunit of the protein in the animal kingdom during the course of evolution, as illustrated in Artemia franciscana by the analysis of its amino acid sequence, which is about 80 % similar to the vertebrate sequence (Macias et al, 1991), whereas there is approximately 90 % similarity between birds, fish and mammals (Schull et al, 1985;Kawakami et al, 1985;Takeyasu et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

et al. 2004

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The ontogeny of osmoregulation was examined in the branchial cavity of embryonic and early postembryonic stages of the crayfish Astacus leptodactylus maintained in freshwater, at the sub-cellular level through the detection of the Na + ,K + -ATPase. The embryonic rate of development was calculated according to the eye index (EI) which was 430-450 µm at hatching. The distribution of the enzyme was identified by immunofluorescence microscopy using a monoclonal antibody IgGα 5 raised against the avian α-subunit of the Na + ,K + -ATPase.Immunoreactivity staining indicating the presence of Na + ,K + -ATPase appeared in the gills of late embryos (EI ≥ 400 µm), i.e. a few days before hatching time, and steadily increased throughout the late embryonic and early postembryonic development. The appearance of the enzyme correlates with the ability to osmoregulate which also occurs late in the embryonic development at EI 410-420 µm and with tissue differentiation within the gill filaments. These observations indicate that the physiological shift from osmoconforming embryos to hyperregulating late embryos and post-hatching stages in freshwater must originate partly from the differentiation in the gill epithelia of ionocytes which are the site of ion pumping, as suggested by the location of Na + ,K + -ATPase. Only the gills were immunostained and a lack of specific staining was noted in the lamina and the branchiostegites. Therefore, osmoregulation through Na + active uptake is likely achieved in embryos at the gill level; all the newly-formed gills in embryos function in ion regulation; other parts of the branchial chamber such as the branchiostegites and lamina do not appear to be involved in osmoregulation.2

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Section: Discussionmentioning

confidence: 99%

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

et al. 2004

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“…The nucleotide sequence of these cDNA clones has shown that the two a-subunits differ at 26.4% of their predicted amino acid sequences, suggesting that they would not cross-hybridize under stringent hybridization conditions. Macias et al (1991) had shown that the pArATNal36 insert does not hybridize with the a2 mRNA. O n the basis of these data, two cDNA probes were prepared to study the tissue specificity of expression of the two Na,K-ATPase a-subunits by in situ hybridization: the complete pArATNal36 cDNA insert and a fragment of the 5' end of the a2-subunit that presented a relatively low homology to pArATNal36 and was cloned by reverse transcribed PCR (see Materials and Methods).…”

Section: Resultsmentioning

confidence: 99%

“…Expression of Na,K-ATl?ase a-Subunits in Second-instar Larvae cDNA clones have been isolated that code for two different Artemia Na,K-ATPase a-subunits: the pArATNal36 cDNA clone (Macias et al, 1991) and several overlapping cDNA clones coding for the a2-subunit ( B a x t e r -h e et al, 1989). The nucleotide sequence of these cDNA clones has shown that the two a-subunits differ at 26.4% of their predicted amino acid sequences, suggesting that they would not cross-hybridize under stringent hybridization conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Biochemical and electrophoretic studies performed in Artemia have allowed identification of two different a-subunits and one P-subunit (Peterson et al, 1978). Complementary DNA clones have also been isolated that code for one P-subunit and two different a-subunits (Macias et al, 1991;Bhattacharyya et al, 1990;Baxter-Lowe et al, 1989). The amino acid sequences predicted from two a-subunit cDNA clones are only 74% identical, showing that they are encoded by two different genes.…”

Section: Introductionmentioning

confidence: 99%

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In situ hybridization analyses of Na, K-ATPase alpha-subunit expression during early larval development of Artemia franciscana.

Escalante

García-Sáez²,

Sastre³

1995

J Histochem Cytochem.

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(4A3444).The spatial pattern of expression of the mRNA encoded by the Na,K-ATPase a-subunit cDNA done pArATNal36 was determined by in situ hybridization of fm, second, and third instar Artma fianuscaaa larvae. This mRNA was expresed at high levels in the salt gland, the antennal gland, and the end of the midgut, which are the three main osmoregulatory organs in Artema at these stages of development. The pattem of expression was similar at the three stages of development analyzed, although the level of expression increased during development, especially in the salt and antennal glands. The expression of the mRNA coding for another Na, K-Aflpase a-subunit isoform, the proposed a2-isoform, was also determined and was shown to be limited to the salt gland. These results suggest that the done pArATNal36 codes for the biwhemicaly defied al-isoform of the Na,K-ATF'ase a-subunit and reinforce the importance of this isoform in osmoregulation at the three larval stages studied. The a2-isoform may also be involved in osmoregulation during the first stages of larval development. (JHistochem Cytochem

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“…Isoformas homólogas da subunidade  dos vertebrados ainda não foram descritas nos invertebrados (EMERY et al, 1998), e mesmo com a identificação de duas isoformas nos camarões Artemia salina e Artemia franciscana, nenhuma corresponde às descritas nos vertebrados (MACÍAS et al, 1991;JORGENSEN & PEDERSEN, 2001). Também foram identificadas duas isoformas para Pachygrapsus marmoratus, a diferença se deve pela inserção de 81 nucleotídeos na porção N-terminal (JAYASUNDARA et al, 2007).…”

Section: Figura 3 Esquema Da Estrutura Das Subunidades Da (Na + K +unclassified

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

Farı́as¹

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Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase α-subunit

Cited by 34 publications

References 38 publications

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

Immunolocalization of Na+,K+-ATPase in the branchial cavity during the early development of the crayfish Astacus leptodactylus (Crustacea, Decapoda)

In situ hybridization analyses of Na, K-ATPase alpha-subunit expression during early larval development of Artemia franciscana.

Caracterização cinética e molecular da (Na+,K+)-ATPase do tecido branquial do caranguejo Cardisoma guanhumi (Latreille,1825).

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