Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae

Sales, Belisa Bordin de; Scheid, Bruna; Gonçalves, Davi L.; Knychala, Marilia M.; Matsushika, Akinori; Bon, Elba P. S.; Stambuk, Boris U.

doi:10.1007/s10529-015-1893-2

Cited by 27 publications

(25 citation statements)

References 52 publications

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“…Indeed, in a recombinant hxt-null strain expression of the HXT1 permease allows consumption of xylose during xylose-glucose co-fermentations (but not with xylose alone), and preventing its ubiquitination (and thus endocytosis) also allows growth of the yeast cells on xylose (Gonçalves et al, 2014;Nijland et al, 2016). Nevertheless, recombinant hxt-null strains with genes for xylose metabolism are suitable platforms for cloning and characterization of novel xylose transporters from other xylose-fermenting yeasts (e.g., de Sales et al, 2015;Saloheimo et al, 2007;Young, Poucher, Comer, Bailey, & Alper, 2011). Sequence comparisons and structural analysis of several sugar transporters have allowed the development of "hexose" permeases (mutant GAL2 or HXT7) with increased affinity (or preference) for xylose (Farwick et al, 2014;Young, Tong, Bui, Spofford, & Alper, 2014).…”

Section: Xylose Transport By S Cerevisiaementioning

confidence: 99%

d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

Patiño

Ortiz

Velásquez

et al. 2019

Yeast

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Xylose is the second most abundant sugar in nature. Its efficient fermentation has been considered as a critical factor for a feasible conversion of renewable biomass resources into biofuels and other chemicals. The yeast Saccharomyces cerevisiae is of exceptional industrial importance due to its excellent capability to ferment sugars. However, although S. cerevisiae is able to ferment xylulose, it is considered unable to metabolize xylose, and thus, a lot of research has been directed to engineer this yeast with heterologous genes to allow xylose consumption and fermentation. The analysis of the natural genetic diversity of this yeast has also revealed some nonrecombinant S. cerevisiae strains that consume or even grow (modestly) on xylose. The genome of this yeast has all the genes required for xylose transport and metabolism through the xylose reductase, xylitol dehydrogenase, and xylulokinase pathway, but there seems to be problems in their kinetic properties and/or required expression. Self‐cloning industrial S. cerevisiae strains overexpressing some of the endogenous genes have shown interesting results, and new strategies and approaches designed to improve these S. cerevisiae strains for ethanol production from xylose will also be presented in this review.

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Section: Xylose Transport By S Cerevisiaementioning

confidence: 99%

d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

Patiño

Ortiz

Velásquez

et al. 2019

Yeast

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“…Mainly discuss the expression of carbohydrates transporters in yeast are focused to improve different substrates and in the modification of specific aminoacides into transporters to regulate the affinity, order to alleviate transport inhibition by sugar concentration. The capacity to co-transport glucose and xylose into yeast has remained a technical challenge in the field [11,29]. Due to the lack of an endogenous xylose transporter in Saccharomyces cerevisiae, the xylose uptake depends on transporter engineering to increase transport rates avoiding glucose-based inhibition, thus enhancing the potential of using lignocellulosic biomass as a feedstock for yeast [11,29].…”

Section: Applications In Biotechnologymentioning

confidence: 99%

“…The capacity to co-transport glucose and xylose into yeast has remained a technical challenge in the field [11,29]. Due to the lack of an endogenous xylose transporter in Saccharomyces cerevisiae, the xylose uptake depends on transporter engineering to increase transport rates avoiding glucose-based inhibition, thus enhancing the potential of using lignocellulosic biomass as a feedstock for yeast [11,29]. Besides of to the generation of fuels, the production of value-added chemicals from renewable biomass has been widely studied.…”

Section: Applications In Biotechnologymentioning

confidence: 99%

Secretion Mechanism across Wall

López-Vargas¹,

Kutralam-Muniasamy²,

Reyes³

et al. 2018

The Yeast Role in Medical Applications

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Yeast organisms are widely explored by humans for different biotechnological applications. During their growth, they need to adapt and interact themselves with the environment medium. For this purpose, organisms uptake nutrients and at the same time secrete different molecules include proteins to extracellular medium. This phenomenon requires the use of specialized structures to regulate entry and exit of molecules called transporters. Two transporters, namely Proteins and Vesicles, are specialized in translocating molecules in and out across the wall. The knowledge of these systems is important and served to bring novel applications of yeast. Taking together, this book chapter is divided into two parts: at first, it primarily accounts on few examples of protein (carbohydrates and peroxisome proteins) and vesicle (intracellular and extracellular vesicles) transporters of yeasts. Second, it deals with the recent advances of yeast applications in diverse area of science.

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“…This limitation is mainly due to the deterioration in xylose utilization efficiency during co-fermentation. Therefore, to overcome this problem, transporter engineering has been applied to boost the xylose import into cells by introducing heterologous pentose transporters or overexpressing homologous pentose-switchable hexose transporters [4][5][6][7]. Nevertheless, the co-fermentation performance of transporter-engineered S. cerevisiae strains in the sequential utilization of glucose and xylose remains suboptimal despite significantly increasing xylose uptake [8,9].…”

Section: Introductionmentioning

confidence: 99%

Improved simultaneous co-fermentation of glucose and xylose by Saccharomyces cerevisiae for efficient lignocellulosic biorefinery

Tran

Gong

et al. 2020

Biotechnol Biofuels

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Background: Lignocellulosic biorefinery offers economical and sustainable production of fuels and chemicals. Saccharomyces cerevisiae, a promising industrial host for biorefinery, has been intensively developed to expand its product profile. However, the sequential and slow conversion of xylose into target products remains one of the main challenges for realizing efficient industrial lignocellulosic biorefinery. Results: In this study, we developed a powerful mixed-sugar co-fermenting strain of S. cerevisiae, XUSEA, with improved xylose conversion capacity during simultaneous glucose/xylose co-fermentation. To reinforce xylose catabolism, the overexpression target in the pentose phosphate pathway was selected using a DNA assembler method and overexpressed increasing xylose consumption and ethanol production by twofold. The performance of the newly engineered strain with improved xylose catabolism was further boosted by elevating fermentation temperature and thus significantly reduced the co-fermentation time by half. Through combined efforts of reinforcing the pathway of xylose catabolism and elevating the fermentation temperature, XUSEA achieved simultaneous co-fermentation of lignocellulosic hydrolysates, composed of 39.6 g L −1 glucose and 23.1 g L −1 xylose, within 24 h producing 30.1 g L −1 ethanol with a yield of 0.48 g g −1. Conclusions: Owing to its superior co-fermentation performance and ability for further engineering, XUSEA has potential as a platform in a lignocellulosic biorefinery toward realizing a more economical and sustainable process for large-scale bioethanol production.

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Cloning novel sugar transporters from Scheffersomyces (Pichia) stipitis allowing d-xylose fermentation by recombinant Saccharomyces cerevisiae

Abstract: Cloning novel sugar transporters not previously identified in the S. stipitis genome using an hxt-null S. cerevisiae strain with a high xylose-utilizing pathway provides novel promising target genes for improved lignocellulosic ethanol production by yeasts.

Cited by 27 publications

References 52 publications

d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

d‐Xylose consumption by nonrecombinant Saccharomyces cerevisiae: A review

Secretion Mechanism across Wall

Improved simultaneous co-fermentation of glucose and xylose by Saccharomyces cerevisiae for efficient lignocellulosic biorefinery

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