Cloning, molecular characterization, and expression pattern of FGF5 in Cashmere goat (Capra hircus)

Bao, Wenlei; Yao, Ruiyuan; He, Qiburi; Guo, Zhixin; Chai, Bin; Wang, Y F; Wang, Z G

doi:10.4238/2015.september.22.9

Cited by 4 publications

(4 citation statements)

References 17 publications

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“…In the reproductive system studies, FGF5 showed specific expression in Sertoli cells in mouse testes [36]. Another study demonstrated that FGF5 was expressed extremely higher in the testis compared with those in the liver and pancreas in Cashmere goat, indicating it may be involved in the regulation of spermatogenesis [25]. In our study, FGF5 was verified to be significantly expressed higher in Sertoli cells from males with normal spermatogensis, compared with SCOS patients.…”

Section: Discussionsupporting

confidence: 67%

“…It has been reported that FGF5 acts as a mitogen to stimulate the proliferation of mesenchymal fibroblasts and mesenchymal stem cells [23]. As for the reproductive system, it has been demonstrated that FGF5 was mainly expressed in mouse Sertoli cells and also displayed plenty of expressions in goat testes [24, 25]. However, the function of FGF5 in male reproduction regulation remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation

et al. 2019

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BackgroundSertoli cells are the most important somatic cells contributing to the microenvironment (named niche) for spermatogonial stem cells (SSCs). They produce amounts of crucial growth factors and structure proteins that play essential roles in the complex processes of male SSCs survival, proliferation, and differentiation. It has been suggested that Sertoli cell abnormalities could result in spermatogenesis failure, eventually causing azoospermia in humans. However, to the end, the gene expression characteristics and protein functions of human Sertoli cells remained unknown. In this study, we aimed to evaluate the effect of fibroblast growth factor-5 (FGF5), a novel growth factor downregulated in Sertoli cells from Sertoli cell-only syndrome (SCOS) patients compared to Sertoli cells from obstructive azoospermia (OA) patients, on SSCs.MethodsWe compared the transcriptome between Sertoli cell from SCOS and OA patients. Then, we evaluated the expression of FGF5, a growth factor which is downregulated in SCOS Sertoli cells, in human primary cultured Sertoli cells and testicular tissue. Also, the proliferation effect of FGF5 in mice SSCs was detected using EDU assay and CCK-8 assay. To investigate the mechanism of FGF5, Phospho Explorer Array was performed. And the results were verified using Western blot assay.ResultsUsing RNA-Seq, we found 308 differentially expressed genes (DEGs) between Sertoli cells from SCOS and OA patients. We noted and verified that the expression of fibroblast growth factor-5 (FGF5) was higher in Sertoli cells of OA patients than that of SCOS patients at both transcriptional and translational levels. Proliferation assays showed that rFGF5 enhanced the proliferation of mouse SSCs line C18-4 in a time- and dose-dependent manner. Moreover, we demonstrated that ERK and AKT were activated and the expression of Cyclin A2 and Cyclin E1 was enhanced by rFGF5.ConclusionThe distinct RNA profiles between Sertoli cells from SCOS and OA patients were identified using RNA-Seq. Also, FGF5, a growth factor that downregulated in SCOS Sertoli cells, could promote SSCs proliferation via ERK and AKT activation.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation

et al. 2019

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“…; Bao et al . ). As a regulator of hair length, FGF5 has been previously reported in a variety of mammals.…”

Section: Resultsmentioning

confidence: 97%

Transcriptomic analysis reveals differentially expressed genes associated with wool length in rabbit

et al. 2018

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Rabbit fur characteristics are primarily genetically determined traits. We used Illumina high-throughput sequencing technology to assess gene expression in the skin tissues of rabbits derived from a cross between Wanxi Angora rabbits and Rex rabbits, which exhibit differential characteristics of short and long wool respectively, to investigate molecular mechanisms related to wool length determination. To identify key regulatory genes involved in rabbit wool length, genes that were differentially expressed between the long- and short-wool rabbits based on a P-value < 0.05 and log |fold change| > 1 were characterized. A total of 798 genes were up-regulated and 523 were down-regulated in the long-wool group compared to expression levels in the short-wool group, and these genes were annotated with GO terms and KEGG pathways, revealing wool-development-related biological functions. The Wnt, Hedgehog and TGF-β signaling pathways, which are related to cell proliferation, fibroblast proliferation and hair follicle regulation respectively, were identified. The expression levels of eight genes were validated by RT-qPCR. In addition, an interaction network was constructed to show the regulatory relationships among the differentially expressed genes. In this study, we found that FGF5, WNT5A, BMP4 and BMP7 showed significant differential expression between the two groups. These transcriptomic profiling results provide comprehensive gene expression information for improving understanding of the molecular mechanisms involved in the growth and development of rabbit wool.

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“…Interestingly, all of the proposed or candidate genes responsible for hair length are attributed to fibroblast growth factor 5 (FGF5). So far, a number of spontaneous mutations of FGF5 associated with hair length have been identified in humans , mice , dogs , cats , donkeys , goats and hamsters . It is indicative that the FGF5 gene is the leading candidate for hair length variation.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9‐mediated loss of FGF5 function increases wool staple length in sheep

Liu²,

Zhang³

et al. 2017

The FEBS Journal

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Fibroblast growth factor 5 (FGF5) regulates hair length in humans and a variety of other animals. To investigate whether FGF5 has similar effects in sheep, we used clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated 9 (Cas9) to generate loss-of-function mutations with the FGF5 gene in Chinese Merino sheep. A total of 16 lambs were identified with genetic mutations within the targeting locus: 13 lambs had biallelic modifications and three lambs had monoallelic modifications. Characterization of the modifications revealed that 13 were frameshift mutations that led to premature termination, whereas the other three were inframe deletions. Thus, CRISPR/Cas9 efficiently generated loss-of-function mutations in the sheep FGF5 gene. We then investigated the effect of loss of FGF5 function on wool traits in 12 lambs and found that wool staple length and stretched length of genetically modified (GM) yearling sheep were significantly longer compared with that of wild-type (WT) control animals. The greasy fleece weight of GM yearling sheep was also significantly greater compared with that of WT sheep. Moreover, the mean fiber diameter in GM sheep showed no significant difference compared with WT sheep, suggesting that the increase in greasy fleece weight was likely attributed to the increase in wool length. The results of this study suggest that CRISPR/Cas9-mediated loss of FGF5 activity could promote wool growth and, consequently, increase wool length and yield.

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Cloning, molecular characterization, and expression pattern of FGF5 in Cashmere goat (Capra hircus)

Cited by 4 publications

References 17 publications

Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation

Fibroblast growth factor-5 promotes spermatogonial stem cell proliferation via ERK and AKT activation

Transcriptomic analysis reveals differentially expressed genes associated with wool length in rabbit

CRISPR/Cas9‐mediated loss of FGF5 function increases wool staple length in sheep

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