Cloning, functional expression and tissue distribution of rabbit αla-adrenoceptor

Miyamoto, Sayako; Taniguchi, Tadatsugu; Suzuki, Fumiko; Takita, Manabu; Kosaka, Nobuyuki; Negoro, Eiju; Okuda, Tomoyuki; Kosaka, Hirotaka; Murata, Satoshi; Nakamura, Seigo; Akagi, Yoshio; Oshita, Masafumi; Watanabe, Yoshinari; Muramatsu, Ikunobu

doi:10.1016/s0024-3205(97)00194-x

Cited by 10 publications

(11 citation statements)

References 14 publications

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“…The antagonistic potency of tamsulosin in prostatic strips from pigmented rabbits (9.11) is similar to that obtained in binding studies on cloned rabbit a 1a -ADRs (9.40). 17 On this basis, we conclude that PE-induced contraction of prostatic smooth muscle in pigmented rabbits is mediated by a 1 -ADRs. These are most likely the a 1a -ADR subtype, as previously claimed.…”

Section: Discussionmentioning

confidence: 70%

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Palea

Chang

Rekik

et al. 2008

Journal of Cataract and Refractive Surgery

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Tamsulosin was more effective than alfuzosin at blocking adrenergic contraction of the iris dilator muscle in pigmented rabbits. Both drugs were less potent in the iris than in the prostate, which suggests that an additional iris receptor could be involved. If valid in humans, our results suggest that attainable plasma concentrations of tamsulosin are able to antagonize iris dilator smooth muscle contraction, whereas those of alfuzosin are not. This could explain the higher frequency of intraoperative floppy-iris syndrome in patients treated with tamsulosin than with alfuzosin.

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Section: Discussionmentioning

confidence: 70%

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Palea

Chang

Rekik

et al. 2008

Journal of Cataract and Refractive Surgery

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“…These molecularly defined receptors (signified by the lowercase subscripts), correspond to the pharmacologically defined α "A -, α "B -and α "D -adrenoceptors (signified by the uppercase subscripts). However, none of the pig α " -adrenoceptor subtypes have been cloned, while in the rabbit only the α "a -and α "d -subtypes have been cloned Miyamoto et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Characterization of α1-Adrenoceptor Subtypes in the Eye

Wikberg-Matsson

Uhlén

Wikberg

2000

Experimental Eye Research

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“…However, the corresponding gene has not been identified yet. Being widely distributed in heart, liver, lower urinary tract tissues, vascular and ocular smooth muscle tissues, α 1 ‐adrenoceptors play an important physiological role in peripheral organs ( Lefkowitz & Caron, 1988 ; Minneman, 1988 ; Muramatsu, 1992 ; Ford et al ., 1996 ; Miyamoto et al ., 1997 ; Suzuki et al ., 1997 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of α₁‐adrenoceptors in the rabbit iris: pharmacological characterization and expression of mRNA

Nakamura

Taniguchi

Suzuki

et al. 1999

British J Pharmacology

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1 Subtypes of a 1 -adrenoceptor in rabbit iris have been examined in functional, binding and molecular biological experiments. 2 In functional studies, exogenous and endogenous noradrenaline produced contractions of the iris dilator muscle. The contractile responses to noradrenaline were competitively antagonized by a range of a 1 -adrenoceptor antagonists (pA 2 values): prazosin (8.1), WB4101 (8.2), BMY7378 (5.9), YM617 (9.5), JTH-601 (8.8), HV723 (7.8) and KMD-3213 (9.8). The same order of inhibitory potency was seen in the adrenergic responses to electrical stimulation. This a nity pro®le corresponds well to that of the putative a 1L -adrenoceptor, which has been proposed in lower urinary tract tissues. 3 In binding studies on rabbit iris membrane however, prazosin, KMD-3213 and WB4101 displayed high a nity (pK d or pK i : 9.6, 10.3, 9.6, respectively), and BMY7378 displayed low a nity (pK i : 6.9). These results show that the binding sites typically correspond to a 1A -adrenoceptor subtype in character, and we could not detect the signi®cant amount of a 1L -adrenoceptor subtype. 4 The expression of the three distinct mRNAs that encode proteins of a 1a -, a 1b -and a 1d -adrenoceptors was studied using reverse transcription-polymerase chain reaction (RT ± PCR). RT ± PCR demonstrated the strongest expression of the a 1a -adrenoceptor, weak expression of the a 1b -adrenoceptor and undetectable expression of the a 1d -adrenoceptor. 5 The present study suggests that a 1A -adrenoceptor is a major subtype detectable in binding and RT ± PCR studies in rabbit iris, but that the adrenergic contractions of iris dilator muscle are mediated via activation of a 1 -adrenoceptor subtype having low a nity for prazosin and WB4101.

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Cloning, functional expression and tissue distribution of rabbit αla-adrenoceptor

Cited by 10 publications

References 14 publications

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Characterization of α1-Adrenoceptor Subtypes in the Eye

Evaluation of α₁‐adrenoceptors in the rabbit iris: pharmacological characterization and expression of mRNA

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Cloning, functional expression and tissue distribution of rabbit αla-adrenoceptor

Cited by 10 publications

References 14 publications

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Comparative effect of alfuzosin and tamsulosin on the contractile response of isolated rabbit prostatic and iris dilator smooth muscles

Characterization of α1-Adrenoceptor Subtypes in the Eye

Evaluation of α1‐adrenoceptors in the rabbit iris: pharmacological characterization and expression of mRNA

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Evaluation of α₁‐adrenoceptors in the rabbit iris: pharmacological characterization and expression of mRNA