Cloning, Expression in Yeast, and Functional Characterization of CYP76A4, a Novel Cytochrome P450 of Petunia That Catalyzes (ω-1)-Hydroxylation of Lauric Acid

Tamaki, Katsutomo; Imaishi, Hiromasa; Ohkawa, Hideo; Oono, Kiyoharu; Sugimoto, Mikihiro

doi:10.1271/bbb.69.406

Cited by 8 publications

(6 citation statements)

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“…The closest homolog to KLU with a described catalytic activity is the maize CYP78A1 protein, which, in vitro, catalyzes omega-hydroxylation of a fatty acid (Imaishi et al, 2000). Also, eight of the nine cytochrome P450 genes that are transcriptionally regulated by KLU activity (CYP76C1, 76C2, 76C3, 76C4, 81F3, 86A4, 86A7, and 94B1) are linked to fattyacid modification: omega-hyroxylation of fatty acids has been directly shown for CYP86A4 and CYP86A7 (Duan and Schuler, 2005), whereas CYPs 76C1, 76C2, 76C3, 76C4, 81F3, and 94B1 are all closely related to proteins for which fatty-acid hydroxylation has been shown (Cabello-Hurtado et al, 1998;Pinot et al, 2000;Tamaki et al, 2005). The homology to CYP78A1 and the concerted regulation of these genes by KLU suggest that KLU itself modifies a fatty-acid-related molecule, which could then feedback regulate other enzymatic activities in a biosynthetic pathway.…”

Section: A Distinct Pathway For Controlling Organ Sizementioning

confidence: 99%

Control of Plant Organ Size by KLUH/CYP78A5-Dependent Intercellular Signaling

et al. 2007

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Plant organs grow to characteristic sizes that are genetically controlled. In animals, signaling by mobile growth factors is thought to be an effective mechanism for measuring primordium size, yet how plants gauge organ size is unclear. Here, we identify the Arabidopsis cytochrome P450 KLUH (KLU)/CYP78A5 as a stimulator of plant organ growth. While klu loss-of-function mutants form smaller organs because of a premature arrest of cell proliferation, KLU overexpression leads to larger organs with more cells. KLU promotes organ growth in a non-cell-autonomous manner, yet it does not appear to modulate the levels of known phytohormones. We therefore propose that KLU is involved in generating a mobile growth signal distinct from the classical phytohormones. The expression dynamics of KLU suggest a model of how the arrest of cell proliferation is coupled to the attainment of a certain primordium size, implying a common principle of size measurement in plants and animals.

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Section: A Distinct Pathway For Controlling Organ Sizementioning

confidence: 99%

Control of Plant Organ Size by KLUH/CYP78A5-Dependent Intercellular Signaling

et al. 2007

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“…Two additional P450s in this C. roseus pathway are tabersonine 16-hydroxylase CYP71D12 [103] and geraniol 10-hydroxylase CYP76B6 [104]. CYP76A4 and CYP76B9 (Petunia hybrida, petunia) hydroxylate lauric acid at its ω-or ω-1 positions [105,106]. CYP71D20 (Nicotiana tabacum, tobacco), the fourth CYP71D subfamily sequence with a function assigned, acts to hydroxylate 5-epi-aristolochene and 1-deoxycapsidiol to form the defense compound capsidiol, a bicyclic, dihydroxylated sesquiterpene [107].…”

Section: Isoflavonoids and Plant Defense Moleculesmentioning

confidence: 99%

Comparison of Cytochrome P450 Genes from Six Plant Genomes

Nelson

Ming

Alam

et al. 2008

Tropical Plant Biol.

134

127

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Plants depend on cytochrome P450 (CYP) enzymes for nearly every aspect of their biology. In several sequenced angiosperms, CYP genes constitute up to 1% of the protein coding genes. The angiosperm sequence diversity is encapsulated by 59 CYP families, of which 52 families form a widely distributed core set. In the 20 years since the first plant P450 was sequenced, 3,387 P450 sequences have been identified and annotated in plant databases. As no new angiosperm CYP families have been discovered since 2004, it is now apparent that the sampling of CYP diversity is beginning to plateau. This review presents a comparison of 1,415 cytochrome P450 sequences from the six sequenced genomes of Vitis vinifera (grape), Carica papaya (papaya), Populus trichocarpa (poplar), Oryza sativa (rice), Arabidopsis thaliana (Arabidopsis or mouse ear's cress) and Physcomitrella patens (moss). An evolutionary analysis is presented that tracks land plant P450 innovation over time from the most ancient and conserved sequences to the newest dicot-specific families. The earliest or oldest P450 families are devoted to the essential biochemistries of sterol and carotenoid synthesis. The next evolutionary radiation of P450 families appears to mediate crucial adaptations to a land environment. And, the newest CYP families appear to have driven the diversity of angiosperms in mediating the synthesis of pigments, odorants, flavors and order-/genus-specific secondary metabolites. Family-by-family comparisons allow the visualization of plant genome plasticity by whole genome duplications and massive gene family expansions via tandem duplications. Molecular evidence of human domestication is quite apparent in the repeated P450 gene duplications occurring in the grape genome.

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“…These include CYP76B6 isolated from C. roseus (Collu et al, 2001;Ginis et al, 2012;Meijer et al, 1993), CYP76B10 from Swertia mussotii (Wang et al, 2010), CYP76A4 from Petunia x hybrida (Tamaki et al, 2005) and CYP76C1 (Mizutani and Ohta, 1998) and CYP76C4 (Hofer et al, 2013) from Arabidopsis thaliana. However, although it has been suggested that GOH hydroxylation could be a common characteristic of CYP76s http://dx.doi.org/10.1016/j.phytochem.2015.08.005 0031-9422/Ó 2015 Elsevier Ltd. All rights reserved.…”

Section: Introductionmentioning

confidence: 99%

“…Due to the difficulties usually encountered in fractioning individual proteins from endogenous CYP pools, characterization of CYPs has primarily depended on gene cloning and heterologous expression in eukaryotic systems, such as Pichia pastoris yeast (Wang et al, 2010), insect cells (Sung et al, 2011), and Saccharomyces cerevisiae (Tamaki et al, 2005). The use of eukaryotic systems allows CYP enzyme activities to be defined in cell lysates or purified microsomes with endogenous or co-expressed CPRs (Sung et al, 2011).…”

Section: Introductionmentioning

confidence: 99%