Cloning, Expression and Purification of Native VP2 Mature Peptide of Canine Parvovirus in Prokaryotic Expression System

Abstract

Canine Parvovirus (CPV-2) is a major cause of gastroenteritis in dogs and wild carnivores worldwide. There are three variants of CPV-2 (CPV2a, CPV2b and CPV2c) circulating worldwide and generated due to mutations at VP2 capsid protein. VP2 is the major immuno dominant capsid protein that determines host specificity and diversity of the virus. In the present study, VP2 gene of Canine parvovirus was cloned and purified in E.coli (DE3) expression system. In-silico analysis of transformed pET22b-VP2 sequences were completely matched with amino acids sequences of the selected mature peptide. Purification of the r-VP2 protein was carried out in native condition by Ni-NTA sepharose column chromatography method. The purified protein can be used for development of diagnostics and prophylaxis for Canine Parvovirus (CPV-2).