Cloning, expression and molecular modeling of the anthocyanidin reductase (FaANR) gene during strawberry fruit development

Mandave, Pallavi; Kuvalekar, Aniket; Mantri, Nitin; Islam, Md Autal; Ranjekar, P. K.

doi:10.17660/th2017/72.3.3

Cited by 4 publications

(1 citation statement)

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“…The genes encoding ANR and LAR enzymes have been cloned and characterized in plants including poplar [ 23 ], buckwheat [ 10 ], lotus [ 24 ] and fruits such as grapevine [ 25 ], strawberry [ 26 ], persimmon [ 27 ] and apple [ 28 ]. The expression pattern of both genes is highly correlated with PA accumulation [ 26 , 27 , 28 , 29 , 30 ]. In apple, the transcript levels of LAR1 and ANR2 genes were significantly correlated with the contents of catechin and epicatechin [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical and Functional Characterization of Anthocyanidin Reductase (ANR) from Mangifera indica L.

et al. 2018

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Mango (Mangifera indica L.) is abundant in proanthocyanidins (PAs) that are important for human health and plant response to abiotic stresses. However, the molecular mechanisms involved in PA biosynthesis still need to be elucidated. Anthocyanidin reductase (ANR) catalyzes a key step in PA biosynthesis. In this study, three ANR cDNAs (MiANR1-1,1-2,1-3) were isolated from mango, and expressed in Escherichia coli. In vitro enzyme assay showed MiANR proteins convert cyanidin to their corresponding flavan-3-ols, such as (−)-catechin and (−)-epicatechin. Despite high amino acid similarity, the recombinant ANR proteins exhibited differences in enzyme kinetics and cosubstrate preference. MiANR1-2 and MiANR1-3 have the same optimum pH of 4.0 in citrate buffer, while the optimum pH for MiANR1-1 is pH 3.0 in phosphate buffer. MiANR1-1 does not use either NADPH or NADH as co-substrate while MiANR1-2/1-3 use only NADPH as co-substrate. MiANR1-2 has the highest Km and Vmax for cyanidin, followed by MiANR1-3 and MiANR1-1. The overexpression of MiANRs in ban mutant reconstructed the biosynthetic pathway of PAs in the seed coat. These data demonstrate MiANRs can form the ANR pathway, leading to the formation of two types of isomeric flavan-3-ols and PAs in mango.

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Section: Introductionmentioning

confidence: 99%

Biochemical and Functional Characterization of Anthocyanidin Reductase (ANR) from Mangifera indica L.

et al. 2018

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Genetic Improvement of Strawberry (Fragaria × ananassa Duchesne)

Ather-uz-Zaman¹,

Al-Khayri

Islam

2018

Advances in Plant Breeding Strategies: Fruits

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Gene expression and metabolite accumulation during strawberry (Fragaria × ananassa) fruit development and ripening

Baldi

Orsucci

Moser

et al. 2018

Planta

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A coordinated regulation of different metabolic pathways was highlighted leading to the accumulation of important compounds that may contribute to the final quality of strawberry fruit. Strawberry fruit development and ripening involve complex physiological and biochemical changes, ranging from sugar accumulation to the production of important volatiles compounds that contribute to the final fruit flavor. To better understand the mechanisms controlling fruit growth and ripening in cultivated strawberry (Fragaria × ananassa), we applied a molecular approach combining suppression subtractive hybridization and next generation sequencing to identify genes regulating developmental stages going from fruit set to full ripening. The results clearly indicated coordinated regulation of several metabolic processes such as the biosynthesis of flavonoid, phenylpropanoid and branched-chain amino acids, together with glycerolipid metabolism and pentose and glucuronate interconversion. In particular, genes belonging to the flavonoid pathway were activated in two distinct phases, the first one at the very early stages of fruit development and the second during ripening. The combination of expression analysis with metabolomic data revealed that the functional meaning of these two inductions is different, as during the early stages gene activation of flavonoid pathway leads to the production of proanthocyanidins and ellagic acid-derived tannins, while during ripening anthocyanins are the main product of flavonoid pathway activation. Moreover, the subtractive approach allowed the identification of different members of the same gene family coding for the same or very similar enzymes that in some cases showed opposite regulation during strawberry fruit development. Such regulation is an important trait that can help to understand how plants specifically channel metabolic intermediates towards separate branches of a biosynthetic pathway or use different isoforms of the same enzyme in different organs or developmental stages.

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Cloning, expression and molecular modeling of the anthocyanidin reductase (FaANR) gene during strawberry fruit development

Cited by 4 publications

References 50 publications

Biochemical and Functional Characterization of Anthocyanidin Reductase (ANR) from Mangifera indica L.

Biochemical and Functional Characterization of Anthocyanidin Reductase (ANR) from Mangifera indica L.

Genetic Improvement of Strawberry (Fragaria × ananassa Duchesne)

Gene expression and metabolite accumulation during strawberry (Fragaria × ananassa) fruit development and ripening

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