Cloning, expression and characterization of potential immunogenic recombinant hemagglutinin-neuraminidase protein of Porcine rubulavirus

Cuevas-Romero, Julieta Sandra; Rivera-Benítez, José Francisco; Hernández-Baumgarten, E.; Hernández-Jáuregui, P; Vega, Marco; Blomström, Anne-Lie; Berg, Mikael; Baule, Claudia

doi:10.1016/j.pep.2016.08.002

Cited by 6 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In fact, the antigenic prediction of the rHN-PorPV protein compared with published sequences of the HN of PRV showed the conservation of important residues in the active sites independently of the genetic mutation observed to occur through the years. [3,14,33]. In addition, evaluation of a commercial PRV vaccine in piglets of 5 and 6 weeks of age by viral challenge using three different isolates with different genetic homology (100%, 99.7% and 97.6% respectively) has been documented, which showed high efficacy and protection, regardless of the genetic variation of the strain used in the challenge [5].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, three-dimensional modeling showed that the main conformational and functional domains of the rHN-PorPV protein were preserved, results further confirmed with functional assays demonstrating that this protein maintained antigenicity and immunogenicity characteristics similar to native HN-PRV protein. [14].…”

Section: Introductionmentioning

confidence: 99%

“…Despite their demonstrated effectiveness in preventing disease, sometimes-sporadic outbreaks occur in vaccinated farms, opening some biosafety concerns [ 5 ]. Work performed in our laboratory has shown the feasibility to produce two recombinant variants of the PRV HN proteins as candidate antigens, one of them r HN-PorPV becoming an alternative economic and scalable Escherichia coli platform to produce large amounts of safe and effective recombinant subunit vaccines in a short timeframe [ 7 , 14 ]. Thus, three-dimensional modeling showed that the main conformational and functional domains of the r HN-PorPV protein were preserved, results further confirmed with functional assays demonstrating that this protein maintained antigenicity and immunogenicity characteristics similar to native HN-PRV protein [ 14 ].…”

mentioning

confidence: 99%

See 2 more Smart Citations

Immunogenicity of a recombinant hemagglutinin neuraminidase-Porcine rubulavirus produced by Escherichia coli of Porcine rubulavirus gives protective immunity of litter after challenge

Cuevas-Romero

Cerriteño-Sánchez

Lara-Romero

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

Porcine rubulavirus (PRV) is a contagious virus that affects the Mexican swine industry. This work aimed to evaluate the immunogenicity of an rHN-PorPV candidate vaccine on pregnant sows, and the protective efficacy afforded to their 7-day-old suckling piglets against PRV lethal challenge. Three sows were immunized with rHN-PorPV formulated with immunestimulating complex (ISCOMs) and two sows with rHN-PorPV protein alone as well as a mock-immunized pregnant sow (negative control). Quantitative ELISA detected a high concentration of anti-rHN-PorPV Immunoglobulin G (IgG) antibodies in sow sera after the second dose of vaccine administered on day 14 until farrowing, showing viral-neutralizing and cross-neutralization activity against different variants of PRV. Sera samples from piglets of immunized sows (with or without adjuvant), showed high concentrations of IgG antibodies.As expected, piglets from the negative control sow (n=5), exhibited severe signs of disease and 100% of mortality after PRV challenge study. Conversely, 75% and 87.5% of the piglets born from the rHN-PorPV and the rHN-PorPV-ISCOMs-immunized sows (n=8), survived, respectively, showing milder PRV clinical signs. Our data indicate that rHN-PorPV candidate vaccine produced in E. coli induces efficient humoral response in pregnant sows and that the maternally derived immunity provides high protection to suckling piglets against PRV lethal challenge.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Immunogenicity of a recombinant hemagglutinin neuraminidase-Porcine rubulavirus produced by Escherichia coli of Porcine rubulavirus gives protective immunity of litter after challenge

Cuevas-Romero

Cerriteño-Sánchez

Lara-Romero

et al. 2022

The Journal of Veterinary Medical Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…Por ello, se han investigado más opciones, por ejemplo, la posibilidad de emplear proteínas recombinantes de RVP como antígenos para producir una respuesta protectora. Se ha estudiado el uso de la proteína HN expresada en E. coli y Pichia pastoris, las cuales inducen la formación de anticuerpos (91,92) . Estudios de predicción estructural y antigénica demuestran que, además de la proteína HN, la proteína F, NP y M, potencialmente inducirían una respuesta inmunitaria.…”

Section: Enfermedad Del Ojo Azulunclassified

Salud porcina: historia, retos y perspectivas

Rivera-Benítez

Luz-Armendáriz

Gómez-Núñez

et al. 2021

Rev. Mex. Cienc. Pecu.

Self Cite

View full text Add to dashboard Cite

En los sistemas de producción porcina, uno de los puntos críticos que deben ser atendidos con estricto rigor, es la salud de los cerdos. La salud, es un componente estructural del bienestar animal y refleja un estado óptimo de los animales, lo que repercute directamente en un mayor desempeño productivo y mejores condiciones de desarrollo. Uno de los eslabones más frágiles de la salud de los cerdos, es la presencia de enfermedades infecciosas más importantes, las cuales pueden representar pérdidas hasta del 100 % de la producción, por lo cual, debe ser un tema de atención constante, y continuamente vigilado por el Médico Veterinario Zootecnista y los productores, en perfecta coordinación con las autoridades sanitarias oficiales. En la actualidad, la implementación de mejores prácticas en la cadena productiva es de interés para productores y consumidores. El control de las enfermedades infecciosas debe ser un tema de colaboración entre los diferentes actores del entorno y ser considerado un bien público, ya que las repercusiones negativas, pueden ser desde el nivel local hasta mundial. En la presente revisión, se abordará la temática relacionada con las principales enfermedades infecciosas que ponen en riesgo la salud porcina, el impacto, las principales aportaciones realizadas por el Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP) en sus 35 años de vida, específicamente en el Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad (CENID-SAI), anteriormente conocido como el emblemático CENID-Microbiología o Palo Alto.

show abstract

“…The expression of two variants of recombinant HN capable of inducing antibodies in mice has been reported as part of the effort to look for an effective vaccine against PRV [14,15]. Indeed, our research group has demonstrated that murine antibodies generated against recombinant HN proteins can neutralize PRV infection in cell cultures [14].…”

Section: Introductionmentioning

confidence: 99%

Immunoinformatics approach for predicting epitopes in HN and F proteins of Porcine rubulavirus

et al. 2020

View full text Add to dashboard Cite

Porcine rubulavirus (PRV), which belongs to the family Paramyxoviridae, causes blue eye disease in pigs, characterized by encephalitis and reproductive failure in newborn and adult pigs, respectively. There is no effective treatment against PRV and no information on the effectiveness of the available vaccines. Continuous outbreaks have occurred in Mexico since the early 1980s, which have caused serious economic losses to pig producers. Vaccination can be used to control this disease. Searching for effective antigen candidates against PRV, we first sequenced the PAC1 F protein, then we used various immunoinformatics tools to predict antigenic determinants of B-cells and T-cells against the two glycoproteins of the virus (HN and F proteins). Finally, we used AutoDock Vina to determine the binding energies. We obtained the F gene sequence of a PRV strain collected in the early 1990s in Mexico and compared its amino acid profile with previous and more recent strains, obtaining an identity similarity of 97.78 to 99.26%. For the F proteins, seven linear B-cell epitopes, six conformational B-cell epitopes and twenty-nine T-cell MHC class I epitopes were predicted. For the HN proteins, sixteen linear B-cell epitopes, seven conformational B-cell epitopes and thirty-four T-cell MHC class I epitopes were predicted. The ATRSETDYY and AAYTTTTCF epitopes of the HN protein might be important for neutralizing the viral infection. We determined the in silico binding energy between the predicted epitopes on the F and HN proteins and swine MHC-I molecules. The binding energy of these epitopes ranged from-5.8 to-7.8 kcal/mol. The present study aimed to assess the use of HN and F proteins as antigens, either as recombinant proteins or as a series of peptides that could activate different responses of the immune system. This may help identify relevant immunogens, saving time and costs in the development of new vaccines or diagnostic tools.

show abstract

Cloning, expression and characterization of potential immunogenic recombinant hemagglutinin-neuraminidase protein of Porcine rubulavirus

Cited by 6 publications

References 24 publications

Immunogenicity of a recombinant hemagglutinin neuraminidase-<i>Porcine rubulavirus</i> produced by <i>Escherichia coli</i> of <i>Porcine rubulavirus</i> gives protective immunity of litter after challenge

Immunogenicity of a recombinant hemagglutinin neuraminidase-<i>Porcine rubulavirus</i> produced by <i>Escherichia coli</i> of <i>Porcine rubulavirus</i> gives protective immunity of litter after challenge

Salud porcina: historia, retos y perspectivas

Immunoinformatics approach for predicting epitopes in HN and F proteins of Porcine rubulavirus

Contact Info

Product

Resources

About