Cloning, expression and bioactivity of duck BAFF

Zhang, Guan; Ye, Ji-Lin; Dan, Wen-Bing; Yao, Wenjuan; Zhang, Shuangquan

doi:10.1016/j.molimm.2006.05.011

Cited by 33 publications

(12 citation statements)

References 18 publications

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“…As shown in Fig. 6, we observed a dose-dependent response to EGFP/dsBAFF treatment, just like NusA-His-dsBAFF (Guan et al 2007). Saturation was reached if treated with 10 lg/ml of EGFP/ dsBAFF, whilst the negative control protein EGFP (5 lg/ml) or PBS did not have any survival effect on the bursal B cells.…”

Section: Egfp/dsbaff-promoted Survival Activitysupporting

confidence: 53%

“…Our previous studies indicated duck BAFF (dBAFF) plays an important role in survival and proliferation of duck B cells (Guan et al 2007), and little information is available on dBAFF receptors. To aid further research on dBAFF and its receptors, in this study, using enhanced green fluorescent protein (EGFP) (excitation maximum at 488 nm; emission maximum at 507 nm), we constructed a novel fluorescent fusion protein, EGFP/dsBAFF, and demonstrated its application for single-step fluorescence detection assay in the study of dBAFF and its receptors.…”

Section: Introductionmentioning

confidence: 99%

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Construction of bifunctional fusion proteins consisting of duck BAFF and EGFP

Dan

Zhang

et al. 2007

Biotechnol Lett

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We constructed fusion proteins consisting of fluorescence-enhanced green fluorescent protein (EGFP) and soluble domain of duck B-cell-activating factor of the TNF family (dsBAFF). The soluble EGFP/dsBAFF was efficiently expressed in Escherichia coli BL 21 (DE3) and was purified in milligram amounts using metal chellate affinity chromatography. The fusion protein exhibited similar fluorescence spectra with free EGFP and promoted the survival of duck bursal B cells in vitro as well as dsBAFF. EGFP/dsBAFF has shown specific binding to duck BAFF receptors positive-cells and the stained cells could be analyzed with flow cytometry. Thus, the fusion protein represents a readily obtainable source of biologically active dsBAFF that may prove useful in further studies on duck BAFF and its receptors.

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Section: Egfp/dsbaff-promoted Survival Activitysupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Construction of bifunctional fusion proteins consisting of duck BAFF and EGFP

Dan

Zhang

et al. 2007

Biotechnol Lett

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“…Finally, the target protein was purified with His-Bind Columns (Qiagen, Germany), according to the manual. The expression of His 6 -tagged SUMO-zsTWEAK was analyzed by Western blotting with an anti-His 6 -tag mouse antibody (Invitrogen, USA) (Guan et al 2007). …”

Section: Expression Purification Of Recombinant Zstweak and Westernmentioning

confidence: 99%

Molecular Cloning, Phylogenetic Analysis, Tissue Distribution, and Expression of the Cytokine TWEAK in Zebrafish (Danio rerio)

Liang

et al. 2011

Biochem Genet

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“…A fusion protein system pET43.1a (Novagen) designed to give soluble expression in E. coli was used (Guan et al, 2006). After digestion with BamH I and Hind III, the PCR product was cloned into the vector pET43.1a.…”

Section: Construction Of Expression Vector Pet431a-gsaprilmentioning

confidence: 99%