Cloning endangered gray wolves (Canis lupus) from somatic cells collected postmortem

Oh, Hyun Ju; Kim, M K; Jang, Goo; Kim, H.J.; Hong, So Gun; Park, Jung E.; Park, Kwangwoo; Park, C.; Sohn, Sea Hwan; Kim, Dae-Yong; Shin, Namsoo; Lee, Byeong Chun

doi:10.1016/j.theriogenology.2008.04.032

Cited by 92 publications

(49 citation statements)

References 43 publications

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“…Furthermore, their mitochondrial (mt) DNA originated from the oocytes of donor dogs. This result is in line with our previous studies [9,14]. In regard to mtDNA, it is important to note that it is believed that the mtDNA of offspring is transmitted from the mother, not the father.…”

supporting

confidence: 92%

“…Additionally, female gray wolves (Canis lupus) were cloned from a 2-year-old female wolf [9], and male cloned gray wolves from postmortem tissue [14]. This indicates that canine SCNT can be applied to conserve endangered species of the Canidae family.…”

mentioning

confidence: 99%

“…Some of the trials to conserve endangered animals using intra-and interspecies SCNT have failed to produce viable offspring [2,11,18]. However, live cloned offspring, such as normal mouflon [13], african wild cat [4,5] and gray wolves [9,14], have been obtained successfully using SCNT.…”

mentioning

confidence: 99%

“…This study was conducted in accordance with recommendations described in "The Guide for the Care and Use of Laboratory Animals" published by Seoul National University. The surgical approach for collection of in vivo matured oocytes and transfer of cloned embryos to the oviduct was performed in accordance with our studies [7,9,12,14]. In brief, anesthesia was induced with 6 mg/kg ketamine HCl and 1 mg/kg xylazine, and general anesthesia was maintained with 2% isoflurane.…”

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confidence: 99%

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Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using Somatic Cell Nuclear Transfer

Jang

Hong

Kang

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. A recent emerging technology, somatic cell nuclear transfer (SCNT), has been considered for conserving threatened or endangered species. Sapsaree is a native breed in Korea and has been designated as a Natural Monument. The aim of this study was to produce a Sapsaree by SCNT for breed conservation. Donor fibroblasts from a 9-year-old male Sapsaree were placed into the perivitelline spaces of enucleated in vivo matured oocytes and fused electrically. A total of 309 cloned embryos were transferred into the oviducts of 15 naturally synchronized recipients. Two recipients were diagnosed as pregnant, and each delivered one cloned puppy, both of which weighed 530 g. Overall, this study demonstrated that an endangered canine breed can be conserved by SCNT.

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confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using Somatic Cell Nuclear Transfer

Jang

Hong

Kang

et al. 2009

The Journal of Veterinary Medical Science

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“…72(1): 89-92, 2010 Cloning technology using somatic cell nuclear transfer (SCNT) provides valuable opportunities for the fields of biomedical science, agriculture and preservation of endangered animals [2,6,17]. In canids, SCNT has been applied not only to the cloning of companion dogs [12,13,16] but also to the preservation of endangered canine species [14,18]. In some species, cloned offspring produced by SCNT have been reported to show normal reproductive characteristics and successfully produce progeny by natural mating [1,2,6,7,21].…”

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confidence: 99%

Serum Levels of Reproductive Hormones and Ultrasonographic Monitoring of Ovarian Follicles in Female Cloned Dogs

Hong

Park

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. This study investigated the reproductive characteristics in 2 cloned female beagle dogs (Clones A and B) during proestrus and early estrus. The pre-ovulatory estradiol peak occurred 2 days before the pre-ovulatory luteinizing hormone (LH) surge, while the follicle stimulating hormone surge started concomitantly with the LH surge in both cloned dogs. Serum progesterone levels increased during proestrus and estrus and its concentration on the day of the LH surge was 3.59 and 2.71 ng/ml in Clones A and B, respectively. Gradual follicular growth was observed by ultrasonography during proestrus. Although the numbers are limited, these cloned female dogs showed normal ranges of reproductive hormone levels and follicular changes during proestrous and early estrous stages of the cycle.KEY WORDS: cloned dog, estrous cycle, puberty, reproductive endocrinology/hormone, ultrasonography.J. Vet. Med. Sci. 72(1): 89-92, 2010 Cloning technology using somatic cell nuclear transfer (SCNT) provides valuable opportunities for the fields of biomedical science, agriculture and preservation of endangered animals [2,6,17]. In canids, SCNT has been applied not only to the cloning of companion dogs [12,13,16] but also to the preservation of endangered canine species [14,18]. In some species, cloned offspring produced by SCNT have been reported to show normal reproductive characteristics and successfully produce progeny by natural mating [1,2,6,7,21]. To our knowledge, however, there are no studies on the reproductive characteristics of cloned dogs. Accordingly, this study was designed to investigate systemic reproductive characteristics of cloned beagles, specifically their hormonal profiles and ovarian follicular development.Recently, we produced two cloned beagles (designated as Clones A and B) derived from canine fetal fibroblasts [10]. For this study, they were monitored regularly from six months of age for the presence of vulvar swelling and serosanguinous vaginal discharge, which were used as indications of the onset of proestrus. From the first day of proestrus, jugular venous blood samples were collected twice a day for hormonal analysis. Serum was prepared by centrifuging at 3,000 rpm for 20 min and stored at -30C until use for hormone measurement. Additionally, to gain more detailed information regarding the changes of hormones around the time of ovulation, blood samples were collected three times a day after the serum progesterone concentration exceeded 1 ng/ml. Previous studies demonstrated that progesterone can be used to accurately predict ovulation in canines [11,14].For analyzing reproductive hormones, progesterone, estradiol-17 (E 2 ), follicle stimulating hormone (FSH), and luteinizing hormone (LH), the assays were performed with the assistance of the Neodin Veterinary Laboratory, Seoul, South Korea (http://www.vetlab.co.kr). Briefly, serum progesterone concentrations were measured by radioimmunoassay (RIA) using a Coat-a-Count Progesterone Kit (Siemens Medical Solutions Diagnostics, U.S.A.). The kit cont...

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Mitochondrial Inheritance Following Nuclear Transfer: From Cloned Animals to Patients with Mitochondrial Disease

Burgstaller

Chiaratti

2023

Methods in Molecular Biology

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Cloning endangered gray wolves (Canis lupus) from somatic cells collected postmortem

Cited by 92 publications

References 43 publications

Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using Somatic Cell Nuclear Transfer

Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using Somatic Cell Nuclear Transfer

Serum Levels of Reproductive Hormones and Ultrasonographic Monitoring of Ovarian Follicles in Female Cloned Dogs

Mitochondrial Inheritance Following Nuclear Transfer: From Cloned Animals to Patients with Mitochondrial Disease

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