Cloning, chromosome mapping and expression pattern of porcinePLINandM6PRBP1genes

Abstract

-The PAT proteins, named after the three PLIN/ADRP/TIP47 (PAT) proteins, PLIN for perilipin, ADRP for adipose differentiation-related protein and TIP47 for tail-interacting protein of 47 kDa, now officially named M6PRBP1 for mannose-6-phosphate receptor binding protein 1, is a set of intracellular lipid droplet binding proteins. They are localized in the outer membrane monolayer enveloping lipid droplets and are involved in the metabolism of intracellular lipid. This work describes the cloning and sequencing of porcine PLIN and M6PRBP1 cDNAs, the chromosome mapping of these two genes, as well as the expression pattern of porcine PAT genes. Sequence analysis shows that the porcine PLIN cDNA contains an open reading frame of 1551 bp encoding 516 amino acids and that the porcine M6PRBP1 cDNA contains a coding region of 1320 bp encoding 439 amino acids. Comparison of PLIN and M6PRBP1 amino-acid sequences among various species reveals that porcine and bovine proteins are the most conserved. Porcine PLIN and M6PRBP1 genes have been mapped to pig chromosomes 7 and 2, respectively, by radiation hybrid analysis using the IMpRH panel. Expression analyses in pig showed a high expression of PLIN mRNA in adipose tissue, M6PRBP1 mRNA in small intestine, kidney and spleen and ADRP mRNA in adipose tissue, lung and spleen.pig / PLIN / M6PRBP1 / cDNA cloning / chromosome mapping / tissue expression pattern

Porcine perilipin (PLIN) gene: Structure, polymorphism and association study in Large White pigs

“…Tissue expression patterns of the porcine PLIN gene studied by Tao et al (2008) revealed ubiquitous expression of PLIN mRNA, with the highest level found in adipose tissue and low level in other tis-sues (liver, kidney, heart, muscle, lung, spleen, pancreas, intestine, brain, stomach). Tao et al (2008) mapped the PLIN gene by radiation hybrid analysis close to microsatellite SWR1210, which is located at position 82.3 cM on the USDA -USMARC linkage map of porcine chromosome 7 (http://www.…”

“…Tao et al (2008) mapped the PLIN gene by radiation hybrid analysis close to microsatellite SWR1210, which is located at position 82.3 cM on the USDA -USMARC linkage map of porcine chromosome 7 (http://www. marc.usda.gov/genome/swine/marker_list.html).…”

Cloning of Perilipin 2 Gene and Investigating its Expression Level in Porcine Longissimus Muscle

“…Non-ubiquitous PLIN2 expression in pigs has been recently reported by Tao et al [29], which indicated that PLIN2 gene did not express in muscle tissue. Davoli et al [12] also detected PLIN2 gene expression analysis in skeletal muscle of Italian Large White and Italian Duroc pigs divergent for backfat thickness and visible intermuscular fat showed a trend of higher expression level in pigs with higher intermuscular fat.…”