Cloning, Characterization, and the Complete 56.8-Kilobase DNA Sequence of the Human NOTCH4 Gene

Li, Linheng; Huang, Guyang Matthew; Banta, Amy B.; Deng, Yu; Smith, T. M. F.; Dong, Penny; Friedman, Cynthia; Chen, Lei; Trask, Barbara J.; Spies, Thomas A.; Rowen, Lee; Hood, Leroy

doi:10.1006/geno.1998.5330

Cited by 49 publications

(52 citation statements)

References 72 publications

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“…Whether these RNA species correspond to splice variants or arise from a cryptic promoter within introns near the 3' end of the gene has not been de®nitively determined. In a survey of normal human tissue RNA, Li et al (1998) reported a major 6.7 Kb NOTCH-4/INT-3 RNA species and a minor 9.3 Kb RNA species. The latter transcript is the result of incomplete splicing.…”

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

“…We have used primer extension analysis to distinguish between the possibilities that the 1.8 Kb RNA species is a splice variant or is initiatated from a cryptic promoter within a NOTCH-4/INT-3 intron. A 37-mer oligonucleotide in exon 27, corresponding to 50417-50453 bp of the complete genomic DNA sequence (Li et al, 1998), was end-labeled with g-32 P-ATP and used for primer extension analysis on SW480 RNA. As shown in Figure 3, two potential transcription start sites (50314 and 50333 bp of NOTCH-4/INT-3 genomic DNA (Li et al, 1998)) were detected in intron 26.…”

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

“…A 37-mer oligonucleotide in exon 27, corresponding to 50417-50453 bp of the complete genomic DNA sequence (Li et al, 1998), was end-labeled with g-32 P-ATP and used for primer extension analysis on SW480 RNA. As shown in Figure 3, two potential transcription start sites (50314 and 50333 bp of NOTCH-4/INT-3 genomic DNA (Li et al, 1998)) were detected in intron 26. To provide evidence that expression of the 1.8 NOTCH-4/INT-3 RNA species in the testis and all of the positive tumor cell lines is initiated within intron 26 we carried out a reverse transcriptase-polymerase chain reaction (RT ± PCR) analysis of RNA from some of the cell lines tested by Northern blot analysis.…”

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

“…This methionine residue corresponds to the twelfth residue of the ®rst 31 amino acid Ankyrin repeat of the NOTCH-4/INT-3 ICD. To determine whether this potential translation start signal is active, the segment of NOTCH-4/INT-3 corresponding to the coding region of the 1.8 Kb RNA species (nucleotides 4855 ± 6102 of the full-length mRNA (Li et al, 1998)) was cloned into the NheI and XhoI sites of the pcDNA3.1(7) expression vector (Invitrogen). Using this expression vector in the TNT Coupled Transcription/Translation System (Promega) a 43 kDa product was detected by SDS ± PAGE analysis ( Figure 5a).…”

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

“…In humans, NOTCH-4/INT-3 has been reported to be normally expressed in all adult tissues as a major 6.5 Kb RNA species as well as a minor 9.0 Kb splice variant (Li et al, 1998). We initiated a survey of human breast, colon, and lung carcinoma cell lines for evidence of the expression of RNA species from genetically`activated' NOTCH-4/INT-3.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Identification of a novel NOTCH-4/INT-3 RNA species encoding an activated gene product in certain human tumor cell lines

Imatani

Callahan

2000

Oncogene

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Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

Section: Notch-4/int-3 Rna Expression In Normal Human Tissues and Tummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification of a novel NOTCH-4/INT-3 RNA species encoding an activated gene product in certain human tumor cell lines

Imatani

Callahan

2000

Oncogene

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Notch Genes

Loomes¹,

Kusumi²,

Spinner³

2002

Wiley Encyclopedia of Molecular Medicine

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Association of six polymorphisms of the NOTCH4 gene with schizophrenia in the Japanese population

Tochigi

Zhang

Umekage

et al. 2004

American J of Med Genetics Pt B

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The NOTCH4 gene is located at 6p21.3 and involved in the development and patterning of the central nervous systems. Recently, Wei and Hemmings [2000] observed that the gene was associated with schizophrenia. Subsequent to the report, several studies investigated the gene in schizophrenia, with controversial and inconclusive results. In the present study, we investigated six polymorphisms (SNPs 1-5 and a CTG repeat) of the gene in Japanese subjects with schizophrenia (n = 284) and the same number of controls. The polymorphisms include SNP5, which has been observed to be associated with schizophrenia in a Chinese population and two new SNPs 3-4 adjacent to SNP5, in addition to the SNPs 1-2 and the CTG repeat, which were suggested for the association with the disease in the previous study. As a result, no significant difference in genotypic distributions or allelic frequencies of the six polymorphisms of the gene was observed between the patients and the controls. Also, no significant difference was found in frequencies of haplotypes of the six polymorphisms between the patients and the controls. However, the distribution of SNP2 was significantly deviated from Hardy-Weinberg equilibrium in the patients (P = 0.000986), not in the controls, which could be a chance or due to an association of SNP2 with the disease. In conclusion, the present study provided no clear evidence for an association between the NOTCH4 gene and schizophrenia in the Japanese population.

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Cloning, Characterization, and the Complete 56.8-Kilobase DNA Sequence of the Human NOTCH4 Gene

Cited by 49 publications

References 72 publications

Identification of a novel NOTCH-4/INT-3 RNA species encoding an activated gene product in certain human tumor cell lines

Identification of a novel NOTCH-4/INT-3 RNA species encoding an activated gene product in certain human tumor cell lines

Notch Genes

Association of six polymorphisms of the NOTCH4 gene with schizophrenia in the Japanese population

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