Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts

Zhang, Haifeng; Fu, Gang; Zhang, Dawei

doi:10.4014/jmb.1404.04039

Cited by 24 publications

(16 citation statements)

References 30 publications

(35 reference statements)

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“…In the present study, the optimal pH and temperature on the lytic activity of r Ps lysC were measured. The existence of a wide range of optimal conditions for the activity of r Ps lysC was in accordance with previous studies (Buonocore et al, ; Yu et al, ; Zhang, Fu, & Zhang, ), but with the species‐specific characteristics. In conclusion, a newly identified C‐type lysozyme was characterized from P. sinensis, and their expression patterns and antimicrobial activities were also investigated.…”

Section: Discussionsupporting

confidence: 91%

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Yuan

et al. 2019

Aquac Res

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Lysozymes play vital roles in humoural immune response against bacterial invasion by its lytic activity. In the present study, a new C‐type lysozyme was identified and characterized from Chinese soft‐shelled turtle Pelodiscus sinensis. The full‐length cDNA of PslysC was of 923 bp, encoding a polypeptide of 148 amino acid residues. The multiple alignments and phylogenetic relationship analysis revealed the highly enzyme‐related conserved residues. The real‐time PCR analysis suggested that PslysC was constitutively expressed in a wide range of tissues with highest level in blood cells and liver. The expression of PslysC could be significantly up‐regulated under Aeromonas jandaei infection and ammonia exposure, while no significant changes were found under Poly I:C infection. The rPslysC protein was expressed in E. coli and purified by Ni‐NTA. The optimal pH and temperature for rPslysC protein lytic activities were determined at pH 7 and 30℃. rPslysC can inhibit the growth of eight kinds of Gram‐negative bacteria, and three kinds of Gram‐positive bacteria. The binding activity of rPslysC to different microbial polysaccharides and microorganism was analysed. The results showed that rPslysC could bind to selected bacteria, and exhibit a strong binding activity to lipopolysaccharide and peptidoglycan, but a weak binding activity to β‐glucan. This suggests that the binding activity might be the major mechanism of action to realize the antibacterial activity. The present study will provide helpful evidence to further understand the innate immunity of P. sinensis, and the interaction mechanisms of C‐type lysozymes with bacterial membranes.

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Section: Discussionsupporting

confidence: 91%

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Yuan

et al. 2019

Aquac Res

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“…The produced lysozyme was resistant to pepsin and trypsin, to some extent at, 40 °C, and efficiently active in the pH range between 3 and 9 and from 20° to 60 °C, respectively. The promising properties of this preparation [ 76 ] as a food or feed additives was also found for the fungal lysozyme isolated from Trichoderma reesei , showing an antimicrobial activity improved at the acidic pH (<6.5) [ 77 ].…”

Section: Sourcesmentioning

confidence: 99%

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

Ferraboschi

Ciceri

Grisenti³

2021

Antibiotics

181

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Lysozyme is a ~14 kDa protein present in many mucosal secretions (tears, saliva, and mucus) and tissues of animals and plants, and plays an important role in the innate immunity, providing protection against bacteria, viruses, and fungi. Three main different types of lysozymes are known: the c-type (chicken or conventional type), the g-type (goose type), and the i-type (invertebrate type). It has long been the subject of several applications due to its antimicrobial properties. The problem of antibiotic resistance has stimulated the search for new molecules or new applications of known compounds. The use of lysozyme as an alternative antibiotic is the subject of this review, which covers the results published over the past two decades. This review is focused on the applications of lysozyme in medicine, (the treatment of infectious diseases, wound healing, and anti-biofilm), veterinary, feed, food preservation, and crop protection. It is available from a wide range of sources, in addition to the well-known chicken egg white, and its synergism with other compounds, endowed with antimicrobial activity, are also summarized. An overview of the modified lysozyme applications is provided in the form of tables.

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“…The most commonly used expression plasmids [22,55,56,57,58] and their key features such as promoters [59,60,61,62,63], affinity tags [64,65] and selection markers [7] have been extensively reviewed in the literature, primarily focusing on the E. coli prokaryotic expression system. Widely used Bacillus strains [66,67], vectors and promoters have also been reviewed [68,69,70].…”

Section: Production Of Recombinant Proteins In a Prokaryotic Exprementioning

confidence: 99%

The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production

Uhoraningoga¹,

Kinsella²,

Henehan³

et al. 2018

Bioengineering

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The production of high yields of soluble recombinant protein is one of the main objectives of protein biotechnology. Several factors, such as expression system, vector, host, media composition and induction conditions can influence recombinant protein yield. Identifying the most important factors for optimum protein expression may involve significant investment of time and considerable cost. To address this problem, statistical models such as Design of Experiments (DoE) have been used to optimise recombinant protein production. This review examines the application of DoE in the production of recombinant proteins in prokaryotic expression systems with specific emphasis on media composition and culture conditions. The review examines the most commonly used DoE screening and optimisation designs. It provides examples of DoE applied to optimisation of media and culture conditions.

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Cloning, Characterization, and Production of a Novel Lysozyme by Different Expression Hosts

Cited by 24 publications

References 30 publications

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Newly identified C‐type lysozyme in Chinese soft‐shelled turtle (Pelodiscus sinensis) exhibits potent antimicrobial activity

Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic

The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production

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