Cloning, characterization, and expression pattern of Atlantic halibut (Hippoglossus hippoglossus L.) CD4-2, Lck, and ZAP-70

Abstract

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First Demonstration of Antigen Induced Cytokine Expression by CD4-1+ Lymphocytes in a Poikilotherm: Studies in Zebrafish (Danio rerio)

“…This result is similar to earlier findings from other fish species [20, 21, 62, 63]. In addition, previous work in our lab using whole mount in situ hybridization (WISH) with a CD4-1 antisense RNA probe demonstrated that the zfCD4-1 was also expressed within the developing thymus in zebrafish embryos 4 days post fertilization [64].…”

Molecular characterization and expression of ZAP-70 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus

“…In healthy tilapia, the highest expression level of ZAP-70 was detected in thymus, followed by spleen and head kidney, which is similar to what is seen for Atlantic halibut ZAP-70 (Overgard et al 2010). This pattern of expression is consistent with sites of lymphopoiesis in teleost.…”

“…In mammals, ZAP-70 is found to interact through its tandem SH2 domains, with phosphorylated ITAMs within the CD3 chains. These two features are conserved in all ZAP-70 (Christie et al 2010;Nunez Ortiz et al 2014;Overgard et al 2010) and CD3 (Laing and Hansen 2011) sequence characterized in teleosts, respectively, implying the interaction between ZAP-70 and CD3 in mammals is conserved in teleosts. The high conservation of the tandem SH2 domains of On-ZAP-70 agrees with this.…”

“…ZAP-70, the key molecule to be activated following TCRs engagement, plays a critical role in signal transductions of T-cell activation. ZAP-70 molecules have been identified from teleosts such as zebrafish (Christie et al 2010), Atlantic halibut (Overgard et al 2010) and sea bass (Nunez Ortiz et al 2014), but no information is available to date about ZAP-70 in Nile tilapia.…”

“…ZAP-70 has only been cloned from a few species of teleosts including zebrafish Danio rerio (Christie et al 2010), Atlantic halibut Hippoglossus hippoglossus (Overgard et al 2010) and sea bass Dicentrarchus labrax (Nunez Ortiz et al 2014), and the study of fish ZAP-70 expression profile and functional properties is rather limited. Nile tilapia (Oreochromis niloticus) is one of the most important economical fishes and widely cultured throughout the world.…”