Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (Penaeidae)

Zeng, Dingyuan; Lei, Aiying; Chen, X.H.

doi:10.4238/2013.november.22.15

Cited by 8 publications

(3 citation statements)

References 26 publications

(23 reference statements)

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“…Mud crab HP preferentially expressed spMIF1, and also highly expressed spMIF2 and spDDT. High level expression of MIF in HP has also been reported in other crustaceans such as white leg shrimp, mitten crab and mud crab [19][20][21]. The high level expression of mud crab MIF family members in HP and intestine, and of other crustacean MIFs in HP suggests the MIF family members are significant innate immune molecules in mud crab and other crustaceans.…”

Section: Accepted Manuscriptmentioning

confidence: 63%

“…Vertebrate liver is enriched with macrophages and natural killer cells and is a predominant innate immune organ that plays a vital role in the host defense against microorganisms [3,36]. Crab HP is a functional counterpart of vertebrate liver, and has been implicated in host defense in invertebrates [19]. Mud crab HP preferentially expressed spMIF1, and also highly expressed spMIF2 and spDDT.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Macrophage migration inhibitory factor (MIF) family in arthropods: Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in mud crabs, Scylla paramamosain

Huang

Duan

Huang

et al. 2016

Fish & Shellfish Immunology

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The macrophage migration inhibitory factor (MIF) family, consisting of MIF and D-dopachrome tautomerase (DDT) in vertebrates, is evolutionarily ancient and has been found across Kingdoms including vertebrates, invertebrates, plants and bacteria. The mammalian MIF family are chemokines at the top of the inflammatory cascade in combating infections. They also possess enzymatic activities, e.g. DDT catalysis results in the production of 5,6-dihydroxyindole (DHI), a precursor of eumelanin. MIF-like genes are widely distributed, but DDT-like genes have only been described in vertebrates and a nematode. In this report, we cloned a DDT-like gene, for the first time in arthropods, and a second MIF in mud crab. The mud crab MIF family have a three exon/two intron structure as seen in vertebrates. The identification of a DDT-like gene in mud crab and other arthropods suggests that the separation of MIF and DDT preceded the divergence of protostomes and deuterostomes. The MIF family is differentially expressed in tissues of adults and during embryonic development and early life. The high level expression of the MIF family in immune tissues, such as intestine and hepatopancreas, suggests an important role in mud crab innate immunity. Mud crab DDT is highly expressed in early embryos, in megalops and crablets and this coincides with the requirement for melanisation in egg chorion tanning and cuticular hardening in arthropods, suggesting a potential novel role of DDT in melanogenesis via its tautomerase activity to produce DHI in mud crab. The clarification of the presence of both MIF and DDT in this report paves the way for further investigation of their functional roles in immunity and in melanogenesis in mud crab and other arthropods.

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Section: Accepted Manuscriptmentioning

confidence: 63%

Section: Accepted Manuscriptmentioning

confidence: 99%

Macrophage migration inhibitory factor (MIF) family in arthropods: Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in mud crabs, Scylla paramamosain

Huang

Duan

Huang

et al. 2016

Fish & Shellfish Immunology

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“…Another central cytokine is the macrophage migration inhibitory factor (MIF), which was released following infection with the digenean trematode parasite Schistosoma mansoni (46). MIF has also been described from the Pacific white shrimp Litopenaeus vannamei in which it functions as a prominent pro-inflammatory cytokine, which is up-regulated following viral infections (47) and predominantly expressed in blood cells, heart, and hepatopancreas. The Pacific oyster genome encodes an IL-17 like cytokine, which is highly expressed following injection with pathogenic bacteria (48).…”

Section: Signal Moleculesmentioning

confidence: 99%

Evolution of Innate Immunity: Clues from Invertebrates via Fish to Mammals

2014

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Host responses against invading pathogens are basic physiological reactions of all living organisms. Since the appearance of the first eukaryotic cells, a series of defense mechanisms have evolved in order to secure cellular integrity, homeostasis, and survival of the host. Invertebrates, ranging from protozoans to metazoans, possess cellular receptors, which bind to foreign elements and differentiate self from non-self. This ability is in multicellular animals associated with presence of phagocytes, bearing different names (amebocytes, hemocytes, coelomocytes) in various groups including animal sponges, worms, cnidarians, mollusks, crustaceans, chelicerates, insects, and echinoderms (sea stars and urchins). Basically, these cells have a macrophage-like appearance and function and the repair and/or fight functions associated with these cells are prominent even at the earliest evolutionary stage. The cells possess pathogen recognition receptors recognizing pathogen-associated molecular patterns, which are well-conserved molecular structures expressed by various pathogens (virus, bacteria, fungi, protozoans, helminths). Scavenger receptors, Toll-like receptors, and Nod-like receptors (NLRs) are prominent representatives within this group of host receptors. Following receptor–ligand binding, signal transduction initiates a complex cascade of cellular reactions, which lead to production of one or more of a wide array of effector molecules. Cytokines take part in this orchestration of responses even in lower invertebrates, which eventually may result in elimination or inactivation of the intruder. Important innate effector molecules are oxygen and nitrogen species, antimicrobial peptides, lectins, fibrinogen-related peptides, leucine rich repeats (LRRs), pentraxins, and complement-related proteins. Echinoderms represent the most developed invertebrates and the bridge leading to the primitive chordates, cephalochordates, and urochordates, in which many autologous genes and functions from their ancestors can be found. They exhibit numerous variants of innate recognition and effector molecules, which allow fast and innate responses toward diverse pathogens despite lack of adaptive responses. The primitive vertebrates (agnathans also termed jawless fish) were the first to supplement innate responses with adaptive elements. Thus hagfish and lampreys use LRRs as variable lymphocyte receptors, whereas higher vertebrates [cartilaginous and bony fishes (jawed fish), amphibians, reptiles, birds, and mammals] developed the major histocompatibility complex, T-cell receptors, and B-cell receptors (immunoglobulins) as additional adaptive weaponry to assist innate responses. Extensive cytokine networks are recognized in fish, but related signal molecules can be traced among invertebrates. The high specificity, antibody maturation, immunological memory, and secondary responses of adaptive immunity were so successful that it allowed higher vertebrates to reduce the number of variants of the innate molecules originating from both inver...

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Evolution of Immunity

Buchmann

2018

Advances in Comparative Immunology

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Cloning, characterization, and expression of the macrophage migration inhibitory factor gene from the Pacific white shrimp Litopenaeus vannamei (Penaeidae)

Cited by 8 publications

References 26 publications

Macrophage migration inhibitory factor (MIF) family in arthropods: Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in mud crabs, Scylla paramamosain

Macrophage migration inhibitory factor (MIF) family in arthropods: Cloning and expression analysis of two MIF and one D-dopachrome tautomerase (DDT) homologues in mud crabs, Scylla paramamosain

Evolution of Innate Immunity: Clues from Invertebrates via Fish to Mammals

Evolution of Immunity

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