Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIalpha and IIbeta genes of Japanese flounder Paralichthys olivaceus

Srisapoome, Prapansak; Ohira, Tsuyoshi; Hirono, Ikuo; Aoki, Takashi

doi:10.1111/j.1444-2906.2003.00800.x

Cited by 58 publications

(46 citation statements)

References 52 publications

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“…In view of the good outcomes using MHC in marker-assisted selection breeding programs, a range of studies has been undertaken to better define the isolation and polymorphism of MHC molecules. Fish MHC genes have been cloned and identified, including those of zebrafish (Sültmann et al 1994), channel catfish (Godwin et al 1997), cichlid (Málaga-Trillo et al 1998, rainbow trout (Ristow et al 1999), flounder (Srisapoome et al 2004), turbot (Zhang & Chen 2006a), half-smooth tongue sole (Xu et al 2009), yellow croaker (Yu et al 2010), spotted halibut (Li et al 2011), Mi-iuy croaker , Chinese long snout catfish (Shen et al 2011) and Nile tilapia (Pang et al 2013;Zhou et al 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Major histocompatibility complex (MHC) loci encode cellular glycoproteins responsible for presenting self or non-self antigens to T cell receptors, and thereby initiate immune responses in invertebrates (Srisapoome et al 2004;Croisetiere et al 2008). MHC molecules, in general, are divided into two main subgroups according to their chemical structures and molecular functions (Rothbard & Gefter 1991).…”

Section: Introductionmentioning

confidence: 99%

“…MHC class II genes, on the other hand, are expressed in antigen-presenting cells such as B cells, macrophages, monocytes and dendritic cells. The predominant function of these genes is to present foreign peptides derived from extracellular pathogens to helper CD4 + T cells (Srisapoome et al 2004). MHC class II proteins are heterodimers, composed of two peptides (α and β chains).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Sun

Meng

et al. 2013

Biologia

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Major histocompatibility complex (MHC) class II molecules play an important role in the immune response of vertebrates. In this paper, full-length MHC IIA cDNA was isolated from swamp eel (Monopterus albus) by rapid amplification of cDNA ends PCR. The genomic structure, molecular polymorphism, tissue distribution, and immune response of the MHC IIA gene to bacterial challenge were investigated. The full-length cDNA (GenBank accession No.: KC616308) is 1,509 bp in length including an 83 bp-long 5' untranslated region (UTR) and a 709 bp-long 3' UTR, which encoded a 238 amino acids protein. In the 2,339 bp-long MHC IIA genomic DNA, four exons and three introns were identified. Sequence comparison exhibited that the deduced amino acid sequence shared 27.1-66.3% identity with those of other species. Seven alleles were identified from five healthy individuals. Number of alleles per individual diversified from two to five. Five different 5' UTR sequences and two different 3' UTR sequences from one individual may infer the existence of five loci at least. Real-time quantitative PCR demonstrated that swamp eel MHC IIA transcripts were ubiquitously expressed in ten tissues, but the expression level was distinctly different. Significant changes were observed in liver, spleen, kidney and intestine after challenged with pathogenic bacteria Aeromonas hydrophilia.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Sun

Meng

et al. 2013

Biologia

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“…Major histocompatibility complex (MHC) class I molecules are located on the surface of vertebrate nucleated cells, and are involved in presenting foreign peptide products to cytotoxic CD8 + T cells by the degradation of intracellular pathogens (Srisapoome et al, 2004). They consist of one membrane-spanning α chain (heavy chain) produced by MHC genes, and one b chain (light chain) produced by the b 2 -microglobuin (β 2 m) gene.…”

Section: Introductionmentioning

confidence: 99%

Genomic characterization and sequence diversity of the β2-microglobulin gene in the miiuy croaker, Miichthys miiuy

et al. 2015

Genet. Mol. Res.

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ABSTRACT. b 2 -Microglobulin (β 2 m) is related to major histocompatibility complex class I alpha chains, and forms cellsurface glycoproteins that mediate a variety of functions in immune defense. In general, β 2 m has no isoforms and is not polymorphic in higher vertebrates, but polymorphisms between different alleles have been found in some fish species. In this study, full-length β 2 m cDNA and genomic sequences were cloned from the miiuy croaker (Miichthys miiuy). The miiuy croaker β 2 m gene shares many of the same characteristics as other fish species. Three exons and two introns were identified in the miiuy croaker β 2 m gene; these genomic structural features are similar to those present in other fish. The deduced β 2 m amino acid sequence exhibited 34.7-90.1% identity with mammal and teleost β 2 m amino acid sequences. Sequence polymorphism analysis in six individuals identified three alleles that encoded two proteins, confirming that β 2 m polymorphisms exist in this species. Phylogenetic analysis elucidated the evolutionary history of the β 2 m protein among warm-blooded vertebrates and bony fish.

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“…Extensive variability of MHC Class II B genes was discovered in the 1 domain (exon 2), which encodes the functional peptide binding region (PBR) [17,26]. Allelic diversity is critical for resistance to a variety of parasites [27].…”

mentioning

confidence: 99%

MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

Chen

Liu

et al. 2012

Chin. Sci. Bull.

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This study examined genetic variation in the major histocompatibility complex (MHC) Class II B gene in turbot (Scophthalmus maximus) by virulent bacterial pathogen challenge. One hundred fry from each of six families were infected with Edwardsiella tarda by intraperitoneal injection. Family mortality ranged from 28.0% to 83.3%. Complete exon 2 and intron 1 sequences of MHC Class II B genes were amplified from five survivor and five non-survivor individuals per family using the clone-sequence method. Thirty-seven sequences from 60 individuals revealed 37 different alleles, 25 of which were unique to this study. The 25 unique alleles belonged to 16 major allele types. Nine alleles were used to examine the association between alleles and resistance/susceptibility to disease. Five alleles were present in an individual, suggesting a minimum of three loci or copies of the turbot MHC Class II B gene. The rate of non-synonymous substitution (d N ) was 2.30 and 1.58 times higher than synonymous substitution (d S ) in the peptide-binding regions (PBR) and non-PBR in whole families, respectively, which suggested balancing selection on exon 2 of the MHC Class II B gene in turbot. One allele, Scma-DBB1*02, was significantly more prevalent in survivor stock than in non-survivor stock (P=0.001). Therefore, this allele might be associated with resistance to bacteria. A second allele, Scma-DBB1*10, was significantly more prevalent in non-survivor stock (P=0.021), and is likely associated with susceptibility to bacteria. turbot (Scophthalmus maximus), Edwardsiella tarda, major histocompatibility complex II B, polymorphism, resistance, susceptibility

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Cloning, characterization and expression of cDNA containing major histocompatibility complex class I, IIalpha and IIbeta genes of Japanese flounder Paralichthys olivaceus

Cited by 58 publications

References 52 publications

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class IIA gene of swamp eel Monopterus albus

Genomic characterization and sequence diversity of the β2-microglobulin gene in the miiuy croaker, Miichthys miiuy

MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families

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