Cloning, Characterization, and Expression Analysis of Eight Stress‐Related NAC Genes in Tartary Buckwheat

Abstract

Plant‐specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in various stress responses. However, the characteristics and functions of NAC TFs in Tartary buckwheat [Fagopyrum tataricum (L.) Gaertn.], an important pseudocereal crop that is strongly adapted to growth in adverse environments, have not been investigated. Here, eight NAC‐like genes, termed FtNAC2 through FtNAC9, were isolated and characterized from Tartary buckwheat. Amino acid sequence alignment analysis demonstrated that all the FtNAC proteins contain typical NAM domain of the NAC TFs family in the N terminus. Phylogenetic analysis showed that the eight FtNAC TFs were assigned to three abiotic stress‐related functional subgroups. To investigate their roles in response to stresses, the relative expression levels of FtNAC genes were analyzed under abiotic stresses (salt, drought, cold, and ultraviolet B) and exogenous phytohormones (abscisic acid, methyl jasmonate, and salicylic acid) treatments. All the FtNAC genes responded to one or more treatments, among which FtNAC4 and FtNAC7 showed >20‐fold upregulation of messenger RNA levels in response to salt, drought, abscisic acid, and salicylic acid treatments. The expression analysis of the NAC gene family in Tartary buckwheat under abiotic stress open the way to further investigate the molecular mechanism of NAC‐dependent transcriptional control of stress response.

Genome-Wide Identification of DNA Binding with One Finger (Dof) Gene Family in Tartary Buckwheat (Fagopyrum tataricum) and Analysis of Its Expression Pattern after Exogenous Hormone Stimulation

“…Furthermore, when seedlings grew to the three-leaf one-heart stage, they were treated with 100 μM methyl jasmonate (MeJA), 100 μM abscisic acid (ABA), 100 μM salicylic acid (SA), 10 μM indole-3-acetic acid (IAA) and 10 μM gibberellin (GA) by foliar spray and treated with deionised water as a blank control. The second leaf of the seedling was harvested 6 h after treatments [ 42 ] and then quickly frozen in liquid nitrogen. Three biological replicates were performed for each sample.…”

Functional Characterization of PsnNAC036 under Salinity and High Temperature Stresses

“…In addition, overexpression of ANAC069 can decrease plant ROS scavenging capability and proline biosynthesis, while knock down of ANAC069 improves plant salt and osmotic stress tolerance [ 36 ], indicating ANAC069 as a negative regulator of plant stress tolerance. In Arabidopsis, the homologous gene of PsnNAC036 is ANAC07 2 (AT4G27410.2, RD26 ), which has been reported to respond to drought, salinity, ABA, SA, and MeJA [ 37 , 38 ]. In this study, we provide several lines of evidence, showing that the PsnNAC036 can improve plant salinity and heattolerance.…”

Genome-wide identification of MAPK gene family members in Fagopyrum tataricum and their expression during development and stress responses

“…To predict the function of FtMAPK s, functionally identified transcription factors, including MYB ( FtMYB7 , FtMYB9 , FtMYB10 , FtMYB13 and FtMYB17 ) [ 45 ], FtMYB21 [ 46 ], FtWRKY46 [ 47 ], NAC ( FtNAC2 , FtNAC3 , FtNAC4 , FtNAC5 , FtNAC6 , FtNAC7 , FtNAC8 and FtNAC9 ) [ 48 ], bHLH ( FtbHLH1 [ 49 ], FtbHLH3 [ 50 ] and FtbHLH4 [ 51 ]), FtbZIP5 [ 52 ] and FtbZIP83 [ 53 ] were used to analyse the expression pattern of FtMAPK s in Tartary buckwheat. Salt stress transcriptome data were obtained from the NCBI Sequence Read Archive ( http://www.ncbi.nlm.nih.gov/Traces/sra/ ) with the accession number PRJNA528524 [ 54 ].…”