Cloning, Bioinformatics and Expression Analysis of <i>SmWRKY53 </i>Gene in <i>Solanum melongena </i>L

Abstract

In this study, SmWRKY53 gene was selected from the data of high temperture transcriptome and gene expression detection of "Tewangda" seedlings. It was then cloned, bioinformatics and preliminary expression analyzed. The results showed that the open reading frame of SmWRKY53 gene was 1 083 bp, encoding 360 amino acids. The 138-144 amino acids contain a typical "WRKYGQK" conserved domain, and the zinc finger structure is C2HC type, belonging to the Group Ⅲ subfamily. The homology comparison and phylogenetic tree analysis showed that SmWRKY53 was closely related to StWRKY53 of potato (Solanum tuberosum), and the homology is 91.41%. Bioinformatics prediction showed that SmWRKY53 was a basic and unstable hydrophilic protein with no signal peptide and transmembrane domain. The ratio of random coil in the secondary structure of protein was the highest, 63.89%. There was amino acid disorder structural regions in the SmWRKY53 protein. Subcellular localization prediction indicated that the protein was most likely located in the nucleus. Quantitative Real-time PCR results showed that the relative expression levels of SmWRKY53 was the highest in mature stem and the lowest in flesh. The expression of SmWRKY53 in roots, stems and leaves of eggplant seedlings first increased and then decreased, and reached the peak at 3 h under high temperature stress. This study provides the reference for further exploring the function of SmWRKY53 gene.