Cloning, Bioinformatics Analysis and Physiological Function of the Pine Wood Nematode Bxadh2 Gene

Abstract

In previous research, the pine wood nematode Bxadh2 gene significantly increased its expression in pine seedlings inoculated with endophytic Bacillus cereus GD2 and pine wood nematode. To explore pine wood nematode Bxadh2 gene function, we cloned and analyzed its biological information, and we preliminarily studied its physiological function through RNA interference. We found that the Bxadh2 gene’s full CDS length is 1269 bp, which encodes 422 amino acids, and presents a relatively stable hydrophobic protein. The protein encoded by the Bxadh2 gene has no signal peptide or transmembrane structure, and it is an intracellular protein that does not participate in transmembrane movement. The RNAi interference results showed that when the pine wood nematode’s Bxadh2 gene was suppressed, its survival rate and fecundity significantly decreased, indicating that the expression of the Bxadh2 gene was related to the growth and development of pine wood nematodes.

Rapid and Efficient Molecular Detection of Phytophthora nicotianae Based on RPA-CRISPR/Cas12a

“…The supernatant was diluted to 10% and then divided into conical bottles, adding 3g Agar every 200mL and sterilizing at 121 °C for 15 min.) To propagate, Bursaphelenchus xylophilus were cultivated on the mycelium of Botrytis cinerea for 4-5 days at 25 °C, and allowed to breed [37]. The genomic DNA (gDNA) was extracted using the DNA Secure Plant Kit, which was obtained from Beijing Tian Gen Biotech Co., Ltd. (Beijing, China).…”