Cloning and transcription analysis of the nitrate reductase gene from Haematococcus pluvialis

Hou, Lulu; Liu, Feng; Zang, Xiaonan; Zhang, Xuecheng; He, Bian; Ding, Yating; Song, Xinwei; Xiao, Dongfang; Wang, Haitao

doi:10.1007/s10529-016-2283-0

Cited by 8 publications

(2 citation statements)

References 18 publications

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“…4 A, the transcription level of SjNR in young sporophytes showed a transient increase after N starvation. Although this phenomenon is uncommon, it has also been reported in several algae, including green algae Haematococcus pluvialis and Dunaliella salina [ 42 , 43 ], suggesting that this may be a compensation for plants to coping with N deficiency [ 41 ]. It was also found that the transcriptional activity of SjNR induced by nitrate decreased rapidly after reaching the peak value (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta)

Wang

Jiang

2023

BMC Plant Biol

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Background Brown macroalgae dominate temperate coastal ecosystems, and their productivity is typically limited by nitrate availability. As an economically important kelp, Saccharina japonica is the most productive farmed seaweed and needs to be supplemented with sufficient nitrate throughout the cultivation process. However, molecular characterization of genes involved in nitrogen assimilation has not been conducted in brown macroalgae. Results Here, we described the identification of the nitrate reductase (NR) gene from S. japonica (SjNR). Using two different cloning methods for SjNR, i.e. rapid amplification of cDNA ends (RACE) and cDNA cloning alone, a single fragment was obtained respectively. According to results of sequence analysis between these two fragments, the tentative coding sequence in two clones, SjNR-L and SjNR-S, were suggested to represent two transcripts of the single copy SjNR, and the ATG of SjNR-S was located inside the third exon of SjNR-L. In the 5′ upstream sequence of each transcript, promoter core elements, response elements, especially multiple N response elements which occurred in microalgal NR, were all predicted. Further sequence analysis revealed that both transcripts encoded all five domains conserved in eukaryotic plant NRs. RT-qPCR results showed that the transcription level of SjNR in juvenile sporophytes could be significantly induced by nitrate and inhibited by ammonium, which was in line with plant NRs. The recombinant SjNR-L and SjNR-S were all proved to have NR activity, suggesting that the single-copy gene SjNR might be regulated on transcription level based on alternative promoters and multiple transcriptional start sites. Moreover, both NADH and NADPH were found to be able to act as electron donors for SjNR alone, which is the first confirmation that brown algal NR has a NAD(P)H-bispecific form. Conclusion These results will provide a scientific basis for understanding the N demand of kelp in various stages of cultivation and evaluating the environmental remediation potential of kelp in eutrophic sea areas.

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Section: Discussionmentioning

confidence: 99%

Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta)

Wang

Jiang

2023

BMC Plant Biol

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“…High carbon-nitrogen ratio significantly increased the expression of acetate kinase (ACK), leading to the increase of Acetyl-CoA [49]. Studies showed that the addition of NaAC and the decrease of nitrate concentration caused the stress in H. pluvialis resulted in chlorophyll content and Rubisco activity [35,50]. In H. pluvialis, carbon dioxide (CO 2 ) is fixed through C3 pathway, which is one of the functions of plant photosynthesis [37,[51][52][53].…”

Section: Increased Expression Of Genes Related To Carbon Fixation Provide Energy For the Synthesis Of Astaxanthin And Fatty Acids Under Smentioning

confidence: 99%

Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis

et al. 2021

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Astaxanthin Production from Microalgae

Butler

Golan²

2020

Microalgae Biotechnology for Food, Health and High Value Products

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Cloning and transcription analysis of the nitrate reductase gene from Haematococcus pluvialis

Abstract: This study provides a better understanding of nitrate regulation in H. pluvialis.

Cited by 8 publications

References 18 publications

Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta)

Cloning and characterization of nitrate reductase gene in kelp Saccharina japonica (Laminariales, Phaeophyta)

Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis

Astaxanthin Production from Microalgae

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