Cloning and tissue distribution of a fatty acyl Δ6-desaturase-like gene and effects of dietary lipid levels on its expression in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis)

Yang, Ziyi; Guo, Zengjun; Ji, Lianyuan; Zeng, Qing‐Yin; Wang, Yao; Yang, Xiaozhen; Cheng, Yongxu

doi:10.1016/j.cbpb.2013.03.010

Cited by 36 publications

(24 citation statements)

References 28 publications

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“…To the best of our knowledge, no gene-encoding desaturase enzyme that can be unequivocally categorised as front-end desaturase has been reported in crustaceans. In some species of decapod crustaceans, namely Chinese mitten crab Eriocheir sinensis (Yang et al 2013), the mud crab Scylla paramamosain (Lin et al 2017), Pacific white shrimp Litopenaeus vannamei (Chen et al 2017) and Australian red claw crayfish Cherax quadricarinatus (Wu et al 2018), desaturase genes referred to as "front-end desaturases" have been cloned but not functionally characterised. These desaturase-like sequences have some typical frontend desaturase features including a cytochrome b 5 -like domain with a heme-binding motif (HPGG) and multiple membrane-spanning regions.…”

Section: Crustaceansmentioning

confidence: 99%

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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Invertebrates represent a large proportion of the biomass in aquatic ecosystems, particularly in the ocean. In recent years, invertebrates have been well-consolidated models in evolutionary developmental biology research and, consequently, genomic and transcriptomic sequence databases from a plethora of species across the animal kingdom have become available. This has provided an excellent source of evidence confirming that invertebrates operate endogenous mechanisms for PUFA production. The present paper reviews the current knowledge of gene complement and, where possible, the function of desaturases and elongases with pivotal roles in PUFA biosynthesis of aquatic invertebrates. More specifically, this review covers three major enzyme types, namely ωx desaturases, front-end desaturases and elongases, that have been characterised from species of sponges, cnidarians, molluscs, annelids, crustaceans, rotifers, echinoderms and non-vertebrate chordates (amphioxus and sea squirt). These studies have shown that invertebrates operate alternative and unusual pathways of PUFA biosynthesis involving gene families with complex phylogeny and functional diversity. Consequently, research in this area provides potentially valuable molecular tools in the form of genes that can be used in the biotechnological production of n-3 products.

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Section: Crustaceansmentioning

confidence: 99%

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

2018

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“…Dietary lipid is essential to farmed fish and crustaceans (Chen et al, ; Hung, Cho, & Slinger, ; Saoud, Yta, & Ghanawi, ; Suprayudi, Takeuchi, & Hamasaki, ; Wu et al, ). Dietary lipid sources and levels exert great effects on growth performance (Yang et al, , ), survival, feed utilization, antioxidant defense, immunity and disease resistance in different aquatic animals (Ma et al, ; Sheen & Wu, ; Wang, Chen, et al, ). Fish meal and fish oil are the best lipid sources for aquacultural feed.…”

Section: Introductionmentioning

confidence: 99%

A combination of vitamins C and E alleviates oxidized fish oil‐induced hepatopancreatic injury in juvenile Chinese mitten crabEriocheir sinensis

et al. 2019

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Dietary lipid oxidation is a lasting concern to aquafeed manufacturers and aquaculture farmers. Here, we determined if there was an association between oxidized fish oil (OFO) and hepatopancreatic injury in Chinese mitten crab, and evaluated the alleviating effects of a combination of vitamins C and E on hepatopancreatic injury. There were significant reductions in the body weight gain, hepatopancreatic index, and feed efficiency in crabs that received OFO. Diets with OFO also resulted in reduced crude fat contents in the hepatopancreas and muscle tissues, and alternations in the hepatopancreatic fatty acid compositions, especially a reduction in polyunsaturated fatty acids. Moreover, dietary OFO exposure reduced the antioxidant defense and non‐specific immune response, caused hepatopancreatic injury, and even apparent whitening. An ultrastructural examination of the whitened hepatopancreas revealed that hepatopancreatic cells were injured at varying degrees following OFO exposure. The combination of vitamins C and E could efficiently maintain a constant fatty acid profile in the hepatopancreas exposed to OFO, thus alleviating OFO‐induced hepatopancreatic injury. In addition, a biochemical analysis indicated that a combination of vitamins C and E not only improved the antioxidant status by mitigating the harmful effects of oxidized oil, but also modulated non‐specific immune responses in the crab that received OFO. Overall, OFO‐induced hepatopancreatic injury may be alleviated by a dietary combination of vitamins C and E, and the feeding of rancid forage fish or diets that contain highly oxidized oil is likely a cause of hepatopancreatic necrosis that is frequently observed in farmed crabs.

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“…9) Over the past few decades, Á6-desaturase genes have been isolated from various organisms and characterized, including genes from cyanobacterium, 10) fungi, 11,12) plants, 13,14) and animals. 15,16) Furthermore, gene expression analysis has indicated that the transcript levels of Á6-desaturase genes in fungi increase under low temperature treatment, suggesting that these genes are involved in cold acclimation, 17,18) but the effect of environmental stresses on Á6-desaturase gene expression and their roles in stress responses have not been determined for higher plants.…”

Section: Identification and Functional Analysis Of A á6-desaturase Gementioning

confidence: 99%

Identification and Functional Analysis of a Δ6-Desaturase Gene and the Effects of Temperature and Wounding Stresses on Its Expression inMicroula sikkimensisLeaves

Zhang

Chen

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Cloning and tissue distribution of a fatty acyl Δ6-desaturase-like gene and effects of dietary lipid levels on its expression in the hepatopancreas of Chinese mitten crab (Eriocheir sinensis)

Cited by 36 publications

References 28 publications

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

Desaturases and elongases involved in polyunsaturated fatty acid biosynthesis in aquatic invertebrates: a comprehensive review

A combination of vitamins C and E alleviates oxidized fish oil‐induced hepatopancreatic injury in juvenile Chinese mitten crabEriocheir sinensis

Identification and Functional Analysis of a Δ6-Desaturase Gene and the Effects of Temperature and Wounding Stresses on Its Expression inMicroula sikkimensisLeaves

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