Cloning and sequencing of two actin genes from Taenia solium (Cestoda)

Campos, Andrés; Bernard, Philippe; Fauconnier, A; Landa, Abraham; Gómez, E.; Hernández, Roberto; Willms, Kaethe; Laclette, Juan Pedro

doi:10.1016/0166-6851(90)90082-w

Cited by 32 publications

(19 citation statements)

References 26 publications

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“…There is no evidence that any actin gene of these two species is muscle specific (Krause et al 1989;Campos et al 1990). We also included the sequence of the only cloned actin gene from the coelenterate Hydra attenuata (Fisher and Bode 1989).…”

Section: Methodsmentioning

confidence: 99%

Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins

et al. 1992

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Invertebrate actins resemble vertebrate cytoplasmic actins, and the distinction between muscle and cytoplasmic actins in invertebrates is not well established as for vertebrate actins. However, Bombyx and Drosophila have actin genes specifically expressed in muscles. To investigate if the distinction between muscle and cytoplasmic actins evidenced by gene expression analysis is related to the sequence of corresponding genes, we compare the sequences of actin genes of these two insect species and of other Metazoa. We find that insect muscle actins form a family of related proteins characterized by about 10 muscle-specific amino acids. Insect muscle actins have clearly diverged from cytoplasmic actins and form a monophyletic group emerging from a cluster of closely related proteins including insect and vertebrate cytoplasmic actins and actins of mollusc, cestode, and nematode. We propose that muscle-specific actin genes have appeared independently at least twice during the evolution of animals: insect muscle actin genes have emerged from an ancestral cytoplasmic actin gene within the arthropod phylum, whereas vertebrate muscle actin genes evolved within the chordate lineage as previously described.

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Section: Methodsmentioning

confidence: 99%

Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins

et al. 1992

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“…Both 5′ parasite Prx RACE fragments were amplified by PCR using reverse primer Prx6R (5′-AACA TCTTTGAGTTCACCATCGACAA-3′) and forward primer SMARTII (5′-AAGCAGTGGTATCAACGCAGAGTAC GCGGG-3′), following the manufacturer's directions. The 5′ fragment of T. solium actin pAT6 gene was done using the reverse primer PAT6R (5′-AGGGAGGGGAAGACAG CACGAGG-3′) designed from the pAT6 gene (Campos et al 1990) and SMARTII. The resulting band of each PCR reaction was cloned into pCRII and sequenced.…”

Section: Transcription Start Site Determinationmentioning

confidence: 99%

“…Cloning of Ts2-CysPrx and Tc2-CysPrx genes T. solium and T. crassiceps genomic DNA was extracted as described previously (Campos et al 1990). Briefly 1.5 g cysticerci was digested with Proteinase K for 2-3 h at 55°C in TRIS 50 mM, EDTA 1 mM, and sarcosyl 0.5%, followed by centrifugation at 1,000×g for 15 min, phenol/chloroform extractions, and isopropanol precipitation.…”

Section: Biological Materialsmentioning

confidence: 99%

“…1). Further sequence analysis showed that the Inr and TATA sequences of Ts2-CysPrx and Tc2-CysPrx are conserved in the Cestoda genes, such as T. solium actin genes pAT5 and pAT6, as well as to the Echinococcus granulosus actin genes EgactI and EgactII (Campos et al 1990;da Silva et al 1993); moreover, the nucleotide distance between both elements is conserved in all the sequences analyzed (Fig. 2).…”

Section: Transcription Start Sitementioning

confidence: 99%

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Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

2009

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The Taenia genus is capable of living for long periods within its hosts. Reports have shown that this successful establishment is related to its efficient defense mechanisms against host immune response and its high tolerance to oxidative stress. In this work, we describe the genomic sequences of one Taenia solium and Taenia crassiceps typical 2-Cys peroxiredoxins (Ts2-CysPrx, Tc2-CysPrx) genes, which are 94% identical in primary sequence with the typical 2-Cys Prxs catalytic motifs. Both genes have the same genomic architecture, showing a TATA box and Initiator (Inr) sequence in their proximal promoter, two exons split by a 67-bp type III intron and one unique transcription start site located inside the Inr. We show that T. crassiceps cysticerci are highly tolerant to H(2)O(2) presenting a lethal concentration 50 of 3.0 mM and demonstrate that the typical Tc2-CysPrx gene is not induced by H(2)O(2), showing a behavior of an antioxidant housekeeping gene. This study describes for first time the gene structure of a typical 2-Cys Prx in the Taenia genus.

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“…Another cysticercosis-related protein-T. solium paramyosin (AgB) (14) has at least three sequences, SHV, PST, and KAKASAES, similar or homologous to the sequences of phage B1, B16, and A8, respectively. Also, the T. solium actin-derived (15) sequences SVPI and GVMVGM are homologous to SVPI from phage A8, SVPW from phage B5 and SVS-VGM from phage A1. However, there were clones with amino acid sequences not encountered in any reported T. crassiceps or T. solium protein, suggesting that they represent either yet unknown proteins or mimotopes of the linear or conformational epitopes of known proteins.…”

Section: Resultsmentioning

confidence: 98%

Characterization of Cerebrospinal Fluid Antibody Specificities in Neurocysticercosis Using Phage Display Peptide Library

Manoutcharian

Sotelo

Garcı́a

et al. 1999

Clinical Immunology

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Cloning and sequencing of two actin genes from Taenia solium (Cestoda)

Cited by 32 publications

References 26 publications

Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins

Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins

Characterization of one typical 2-Cys Peroxiredoxin gene of Taenia solium and Taenia crassiceps

Characterization of Cerebrospinal Fluid Antibody Specificities in Neurocysticercosis Using Phage Display Peptide Library

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