Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus

Hayakawa, Tetsuo; Xu, Jinfan; Hukuhara, Takehiko

doi:10.1016/0378-1119(96)00297-1

Cited by 30 publications

(18 citation statements)

References 7 publications

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“…The sequences used were : acgp37, AcMNPV GP37 (Wu & Miller, 1989) ; bmgp37, BmNPV GP37 (GenBank accession no. L33180) ; cfgp37, CfMNPV GP37 (U26734) ; opgp37, OpMNPV GP37 (Gross et al, 1993) ; mbgp37 and ldgp37, this paper ; lsgp37, Leucania separata NPV GP37 (AB009614) ; ancuepvfus, AncuEPV fusolin (Mitsuhashi et al, 1997) ; mmepvfus, MmEPV fusolin (Gauthier et al, 1995) ; haepvfus, HaEPV fusolin (Dall et al, 1993) ; psepvfus, PsEPV fusolin (Hayakawa et al, 1996) ; cbepvfus, CbEPV fusolin ; pschib, Pseudoalteromonas Chi B (AF007895) ; and shcbp, Streptomyces halstedii CBP (Garda et al, 1997).…”

Section: Transcription Start Sites Of Mbmnpv and Ldmnpv Gp37 Genesmentioning

confidence: 93%

“…The tripeptide motif His-Gly-Tyr is absolutely conserved near the N terminus of all proteins. This is the mature N terminus of several of the fusolins and the amino acids N-terminal of it are considered to constitute a signal sequence (Dall et al, 1993 ;Hayakawa et al, 1996 ;Mitsuhashi et al, 1997 ;Yuen et al, 1990). LdMNPV GP37 includes two potential signals for N-linked glycosylation, at amino acids 180-182 and 195-197.…”

Section: Mbmnpv and Ldmnpv Gp37 Genesmentioning

confidence: 99%

“…Indeed, there are instances among the EPVs where the spindle bodies are incorporated into the spheroids (Goodwin et al, 1991). In contrast to OBs, the proteins that form the matrix of the spindle body, termed GP37 in baculoviruses and fusolin in EPVs, are clearly related, sharing 30-40 % amino acid sequence identity (Dall et al, 1993 ;Gauthier et al, 1995 ;Gross et al, 1993 ;Hayakawa et al, 1996 ;Liu & Carstens, 1996 ;Mitsuhashi et al, 1997 ;Vialard et al, 1990). Both display a high cysteine content, presumably involved in crystal formation, and many of these proteins are glycoproteins.…”

Section: Introductionmentioning

confidence: 99%

“…There is accumulating evidence that this is the case for fusolin proteins. Hayakawa et al (1996) purified a factor present in Pseudaletia separata (Ps) EPV OB preparations that could enhance Ps nucleopolyhedrovirus (NPV) infection of this insect. Cloning and sequence analysis of this factor revealed it to be a fusolin protein.…”

Section: Introductionmentioning

confidence: 99%

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Identification and expression of two baculovirus gp37 genes.

Phanis

Miller

Cassar

et al. 1999

Journal of General Virology

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The gp37 genes of the Mamestra brassicae and Lymantria dispar multicapsid nucleopolyhedroviruses (MbMNPV and LdMNPV) have been identified and characterized. Both genes were similar to other baculovirus gp37 genes and to entomopoxvirus fusolin genes. Phylogenetic analysis showed that baculovirus gp37 genes and entomopoxvirus fusolin genes form two distinct and wellseparated clades. There was no evidence of recent gene transfer between the two groups. The gp37 genes also showed a distant similarity to bacterial cellulose-and chitin-binding protein genes, but the significance of this is unclear. MbMNPV and LdMNPV gp37 were both transcribed from consensus baculovirus late transcription start sites. MbMNPV gp37 was additionally transcribed from a putative early transcription start site. Tunicamycin treatment of MbMNPVinfected cells confirmed that MbMNPV GP37 is N-glycosylated. Confocal immunofluorescence microscopy revealed that the protein is located exclusively in the cytoplasm, probably in the endoplasmic reticulum.

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Section: Transcription Start Sites Of Mbmnpv and Ldmnpv Gp37 Genesmentioning

confidence: 93%

Section: Mbmnpv and Ldmnpv Gp37 Genesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and expression of two baculovirus gp37 genes.

Phanis

Miller

Cassar

et al. 1999

Journal of General Virology

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“…Moreover, PsEPV fusolin has been identified as the factor of enhancing PuNPV infection and was reported to be present in the virions within PsEPV spheroids, as well as PsEPV spindles (6,8,9,27). AcEPV fusolin produced by a recombinant baculovirus enhanced Bombyx mori NPV (BmNPV) infection in B. mori larvae (Y. Takemoto, W. Mitsuhashi, R. Murakami, K. Miyamoto, and S. Wada, Abstr.…”

mentioning

confidence: 99%

Spindles of an Entomopoxvirus Facilitate Its Infection of the Host Insect by Disrupting the Peritrophic Membrane

Mitsuhashi

Kawakita

Murakami

et al. 2007

J Virol

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The mode of action by which entomopoxvirus (EPV) spindles, proteinaceous crystalline bodies produced by EPVs, enhance EPV infection has not been clarified. We fed Anomala cuprea EPV (AcEPV) spindles to host insects; subsequent scanning electron microscopy revealed the disruption of the peritrophic membranes (PMs) of these insects. The PM is reportedly a barrier against the infection of some insects by viruses. Quantitative PCR of AcEPV DNA in the ectoperitrophic area revealed that PM disruption facilitated the passage of EPVs through the PM toward the initial infection site, the midgut epithelium. These results indicate that EPV spindles enhance infection by EPVs by disrupting the PM in the host insects. Fusolin is almost exclusively the constituent protein of the spindles and is the enhancing factor of the infectivity of nucleopolyhedroviruses (NPVs) and possibly that of EPVs. Spheroid is another type of proteinaceous crystalline structure produced by EPVs. Pseudaletia separata EPV (PsEPV) spheroids reportedly contain considerable amounts of fusolin and enhance NPV infection. We assessed the ability of AcEPV spheroids to enhance EPV infectivity and their effect on the PM and carried out immunological experiments; these experiments showed that AcEPV spheroids contain little or no fusolin and are biologically inactive, in contrasts to the situation in PsEPV.Entomopoxviruses (EPVs) are poxviruses that infect insects of orders such as Coleoptera, Lepidoptera, Orthoptera, and Diptera. Many EPVs form two types of proteinaceous crystalline bodies: spheroids and spindles. The spheroid contains virions, whereas the spindle does not; thus, spheroids are agents of infection. The major constituent proteins of the spheroid and spindle are spheroidin and fusolin, respectively, and these two are the most abundant proteins among those produced by EPVs. Peroral administration of the spindles of a coleopteran EPV, Anomala cuprea EPV (AcEPV), to Bombyx mori and Spilosoma imparilis, lepidopteran hosts of two species of nucleopolyhedroviruses (NPVs), enhances the infectivity of not only the occluded NPVs of these two species (16, 19) but also of a nonoccluded NPV of B. mori (4), although AcEPV itself cannot infect these two insects. However, these are artificial phenomena that occur by experimental combination of the spindles with NPVs; these insects on plants rarely ingest AcEPV spindles that are present in soils, together with the NPVs. Also, this mechanism of enhanced infectivity of NPVs is considered to be worthless for the survival of AcEPVs that cannot infect the insects. NPVs and EPVs are taxonomically distant DNA insect viruses. NPVs belong to the family Baculoviridae, whereas EPVs belong to the Poxviridae. However, AcEPV spindles also enhance the infectivity of AcEPV in its host insect, the larva of the cupreous chafer A. cuprea, strongly suggesting that the natural biological function of EPV spindles is to enhance the infectivity of EPVs (15,20). (17) showed that the peritrophic membrane (PM) of B. mori larvae was disinte...

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Insect-virus relationships: Sifting by informatics

2001

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Several groups of large DNA viruses successfully utilise the rich resource provided by insect hosts. Defining the mechanisms that enable these pathogens to optimise their relationships with their hosts is of considerable scientific and practical importance, but our understanding of the processes involved is, as yet, rudimentary. Here we describe an informatics-based approach that uses comparison of viral genomic sequences to identify candidate genes likely to be specifically involved in this process. We hypothesise that such genes should satisfy two essential criteria, namely, that they should be (i) present in those members of a virus family that infect insects, but absent from those that infect other hosts, and (ii) found in at least two unrelated taxa of insect viruses. These criteria currently identify six groups of viral genes, including one that encodes the fusolin/gp37 proteins. Demonstration that the fusolin/gp37 proteins can enhance oral infectivity of insect viruses provides a primary validation of this approach to the examination of insect-virus relationships.

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Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus

Cited by 30 publications

References 7 publications

Identification and expression of two baculovirus gp37 genes.

Identification and expression of two baculovirus gp37 genes.

Spindles of an Entomopoxvirus Facilitate Its Infection of the Host Insect by Disrupting the Peritrophic Membrane

Insect-virus relationships: Sifting by informatics

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