Cloning and sequencing of the bovine STAT5A cDNA reveals significant sequence divergence with ovine

Schröder, Petra; Meyer, Lutz; Wheeler, Thomas T.; Thiesen, Hans‐Jürgen; Seyfert, Hans‐Martin

doi:10.1016/s0167-4781(98)00054-2

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…For both proteins, the putative DNAbinding (Horvath et al 1995;Schindler et al 1995), SH3 and SH2 domains are highly conserved whereas the COOH-terminal transactivating domain is less conserved (Liu et al 1995;Moriggl et al 1996). Similarity analysis of STAT5A protein sequences showed that the porcine sequence is 97%, 97% and 96% identical to the human (Lin et al 1996), bovine (Schroder et al 1998)) and mouse sequences, respectively. Amino acid sequence identity of 97% between the porcine and human STAT5B (Lin et al 1996) and of 96% between porcine and mouse STAT5B were obtained ).…”

Section: Cloning and Sequence Analysis Of Porcine Stat5a And Stat5bmentioning

confidence: 98%

“…Second strand cDNA was synthesized and a Marathon cDNA adaptor was ligated as recommended by the manufacturer. The 3' end amplification of pig STAT5A and STAT5B mRNA was performed using an upstream primer 5'-GCGAGGCGCT-CAACATGAAATTC-3', which corresponds to bovine STAT5A cDNA between nucleotides 1591 and 1613 (GenBank accession number Z72482) (Schroder et al 1998) and to human STAT5B cDNA sequence between nucleotides 1669 and 1691 (GenBank accession number U47686) (Lin et al 1996). The amplified 3' STAT5A corresponds to a 1.5 kb fragment and the 3' amplified STAT5B corresponds to a 3.4 kb fragment.…”

Section: Rna Extractionmentioning

confidence: 99%

“…STAT5B showed three amino acid variations, which are P153L, R378Q and C392Y. The sequences for STAT5 proteins are from the following sources: pig STAT5A (this study; GenBank accession numbers AF135122), pig STAT5B (this study; GenBank accession numbers AF135123), human STAT5A [ (Lin et al 1996); GenBank accession number U43185], human STAT5B [ (Lin et al 1996); GenBank accession number U47686], bovine STAT5A [ (Schroder et al 1998); GenBank accession number Z72482], mouse STAT5B [ negatively correlated with DNA concentrations in mammary parenchyma (r = -0.42, P < 0.05). Levels of STAT5B mRNA were correlated with parenchymal weight (r = 0.50, P < 0.01), extraparenchymal weight (r = 0.62, P < 0.001) and to a lesser extent with total RNA (r = 0.45, P < 0.05) and the RNA/DNA ratio (r = 0.46, P < 0.05).…”

Section: Stat5a and Stat5b Mrna Levels In Mammary Glandmentioning

confidence: 99%

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Expression levels of STAT5A and STAT5B in mammary parenchymal tissue from Upton-Meishan and Large White gilts

Palin¹,

Beaudry²,

Roberge³

et al. 2002

Can. J. Anim. Sci.

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. 2002. Expression levels of STAT5A and STAT5B in mammary parenchymal tissue from Upton-Meishan and Large White gilts. Can. J. Anim. Sci. 82: 507-518. The implication of STAT5A and STAT5B in mammary gland development and maintenance of lactation is well documented in rodents and humans. However, little is known regarding their roles in mammary gland development during gestation in pigs. We identified and analyzed the complete coding sequences of swine STAT5A and STAT5B and evaluated their mRNA levels in mammary glands of gestating gilts (day 110) in two different breeds, Upton-Meishan and Large White. Sequence analysis revealed a new APASA insertion in the STAT5A amino acid sequence that is in close proximity to residue Tyr 699 and whose phosporylation leads to the activation of target genes' transcription. STAT5A mRNA levels were higher in Upton-Meishan than in Large White. In both breeds, STAT5B mRNA levels were higher than those of STAT5A, which is contrary to what was found in other mammals. A correlation between circulating IGF-I levels and STAT5B mRNA levels in the mammary gland was noticed in the Upton-Meishan breed only. STAT5B mRNA levels in mammary tissue of Large White gilts were highly correlated with extra-parenchymal tissue weight, parenchymal tissue weight, total parenchymal DNA, RNA and RNA/DNA ratio. In Upton-Meishan gilts, correlations were observed only between extra-parenchymal weight and STAT5A and STAT5B mRNA levels. These results indicate that there are significant differences in mRNA levels of STAT5A and STAT5B in the mammary glands of pregnant gilts when compared to other mammals, and between swine breeds.

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Section: Cloning and Sequence Analysis Of Porcine Stat5a And Stat5bmentioning

confidence: 98%

Section: Rna Extractionmentioning

confidence: 99%

Section: Stat5a and Stat5b Mrna Levels In Mammary Glandmentioning

confidence: 99%

See 1 more Smart Citation

Expression levels of STAT5A and STAT5B in mammary parenchymal tissue from Upton-Meishan and Large White gilts

Palin¹,

Beaudry²,

Roberge³

et al. 2002

Can. J. Anim. Sci.

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“…Genomic isolates from STAT5-encoding genes were obtained from previously constructed bovine libraries (Koczan et al 1991) using bovine STAT5A cDNA as a hybridization probe (Schröder et al 1998) using standard procedures. Isolation of a STAT5A harboring cosmid clone has already been described (McCracken et al 1997).…”

Section: Methodsmentioning

confidence: 99%

“…We have previously reported a description of the bovine STAT5A cDNA (Schröder et al 1998) and the identification of polymorphic microsatellites in both genes (McCraken et al 1997;Meyer et al 1999) which can be used to link STAT5 with production traits in cattle breeding populations. However, we had detected several copies of pseudogenes among our first genomic isolations, which possessed near-sequence identify over several kilobases to the true STAT5 genes.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

et al. 2000

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The STAT transcription factors form a family of signal transducers and activators of transcription. We sequenced the bovine STAT5B cDNA and both STAT5-encoding genes, STAT5A and STAT5B, representing the first complete description of any STAT5-encoding gene. DNA fiber FISH hybridization revealed that the genes reside only 40 kbp apart on BTA19. Both genes are segmented into 19 exons and all but two of the homologous exons are of equal size. The genes harbor a central block of nearly identical DNA sequence (97.5% sequence identity over 3373 bp), spanning from intron 5 to intron 9. Isolation and sequencing of the homologous segments from mouse revealed the same unusually high degree of intronic sequence conservation in these segments of the murine STAT5-encoding genes. However, the respective sequences are completely divergent between the two species. A comparison of the inter- and intragenic cDNA sequence preservation at nonsynonymous sites reveals that the DNA-binding domain is under the strongest selection pressure for both intergenic and factor-specific intragenic sequence preservation. The so-called "SH3" segment of the linker domain, in contrast, shows species-specific sequence identity in all but one amino acid residues in both factors, in cattle, human, and mouse. This indicates that the same species-specific selection pressure occurs on the linker domain from both factors, STAT5A and STAT5B. Thus, the comparison of evolutionary selection pressures resting on various domains suggests that the DNA-binding domain might contribute to differential DNA binding of STAT5A and STAT5B factors, while both might interact equally well with other cellular factors through a segment of the linker domain.

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Stat5 phosphorylation status and DNA-binding activity in the bovine and murine mammary glands

Wheeler

Broadhurst

Sadowski³

et al. 2001

Molecular and Cellular Endocrinology

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Cloning and sequencing of the bovine STAT5A cDNA reveals significant sequence divergence with ovine

Cited by 5 publications

References 36 publications

Expression levels of STAT5A and STAT5B in mammary parenchymal tissue from Upton-Meishan and Large White gilts

Expression levels of STAT5A and STAT5B in mammary parenchymal tissue from Upton-Meishan and Large White gilts

Molecular Characterization of STAT5A- and STAT5B-Encoding Genes Reveals Extended Intragenic Sequence Homogeneity in Cattle and Mouse and Different Degrees of Divergent Evolution of Various Domains

Stat5 phosphorylation status and DNA-binding activity in the bovine and murine mammary glands

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