Cloning and Sequencing of Sulfite Reductase   Subunit Gene from Saccharomyces cerevisiae

Hosseini-Mazinani, Seyed Mehdi; Koshikawa, Naoki; Sugimoto, Kazunori; Aoki, Yasuhiro; Arisawa, Mikio

doi:10.1093/dnares/2.1.15

Cited by 3 publications

(1 citation statement)

References 14 publications

(10 reference statements)

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“…As a whole, expression levels were increased by heat-shock treatment ( Figure 2d) and decreased in nutrient-starved or sporulation conditions (Figure 2b and 2e). Remarkable induction of previously reported genes was observed, for instance, MET10 (YFR030w) in the nutrient-starved condition, which agrees well with a previous report (Hosseini-Mazinani et al, 1995).…”

Section: Quantitative Analysissupporting

confidence: 92%

Expression Profiles of Transcripts from 126 Open Reading Frames in the Entire Chromosome VI ofSaccharomyces cerevisiae by Systematic Northern Analyses

Naitou¹,

Hagiwara²,

Hanaoka³

et al. 1997

Yeast

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Chromosome VI of Saccharomyces cerevisiae contains 126 open reading frames (ORFs), and the functions of proteins encoded by 80 ORFs are still unknown. In this report, we have systematically examined the expression profiles of all 126 ORFs on chromosome VI under five kinds of growth conditions by quantitative Northern hybridization. A series of Northern analyses and reverse transcription polymerase chain reactions have revealed that more than 64 novel ORFs are transcribed. Two ORFs (YFL059w and YFR011c) are specifically expressed in the presence of galactose. Two ORFs (YFL012w and YFR032c) are specifically transcribed in sporulation. Six ORFs (YFL049w, YFL035c, YFL010c, YFR006w, YFR010w and YFR017c) are abundantly expressed in many growth conditions. © 1997 John Wiley & Sons, Ltd.

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Section: Quantitative Analysissupporting

confidence: 92%

Expression Profiles of Transcripts from 126 Open Reading Frames in the Entire Chromosome VI ofSaccharomyces cerevisiae by Systematic Northern Analyses

Naitou¹,

Hagiwara²,

Hanaoka³

et al. 1997

Yeast

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Fifteen open reading frames in a 30·8 kb region of the right arm of chromosome VI fromSaccharomyces cerevisiae

Eki

Naitou

Hagiwara

et al. 1996

Yeast

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The nucleotide sequence of cosmid clone 9765, which contains 30·8 kb of the right arm of chromosome VI, was determined. Both strands were sequenced, with an average redundancy of 8·17 per base pair by both dye primer and dye terminator cycle sequencing methods. The G+C content of the sequence was found to be 40·3%. Fifteen open reading frames (ORFs) greater than 100 amino acids and one tRNA‐Tyr gene (SUP6) were detected. Seven of the ORFs were found to encode previously identified genes (HIS2, CDC14, MET10, SMC2, QCR6, PH04 and CDC26). One ORF, 9765orfF010, was found to encode a new member of the Snf2/Rad54 helicase family. Three ORFs (9765orfR002, 9765orfR011 and 9765orfR013) were found to be homologous with Schizosaccharomyces pombe polyadenylate binding protein, Escherichia coli hypothetical 38·1‐kDa protein in the BCR 5′ region, and transcription regulatory protein Swi3, respectively. The sequence may be found in the DDBJ, EMBL and GenBank nucleotide sequence databases under Accession Number D44602.

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Antifungal azoxybacilin exhibits activity by inhibiting gene expression of sulfite reductase

Aoki¹,

Yamamoto²,

Hosseini-Mazinani³

et al. 1996

Antimicrob Agents Chemother

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Azoxybacilin, produced by Bacillus cereus, has a broad spectrum of antifungal activity in methionine-free medium and has been suggested to inhibit sulfite fixation. We have further investigated the mode of action by which azoxybacilin kills fungi. The compound inhibited the incorporation of [35S] sulfate into acid-insoluble fractions of Saccharomyces cerevisiae under conditions in which virtually no inhibition was observed for DNA, RNA, or protein synthesis. It did not interfere with the activity of the enzymes for sulfate assimilation but clearly inhibited the induction of those enzymes when S. cerevisiae cells were transferred from rich medium to a synthetic methionine-free medium. Particularly strong inhibition was observed in the induction of sulfite reductase. Northern (RNA) analysis revealed that azoxybacilin decreased the level of mRNA of genes for sulfate assimilation, including MET10 for sulfite reductase and MET4, the transactivator of MET10 and other sulfate assimilation genes. When activities of azoxybacilin were compared for mRNA and enzyme syntheses from MET10, the concentration required for inhibition of transcription of the gene was about 10 times higher (50% inhibitory concentration = 30 micrograms/ml) than that required for inhibition of induction of enzyme synthesis (50% inhibitory concentration = 3 micrograms/ml). The data suggest that azoxybacilin acts on at least two steps in the expression of sulfite reductase; the transcriptional activation of MET4 and a posttranscriptional regulation in MET10 expression. We conclude that azoxybacilin exhibits antifungal activity by interfering with the regulation of expression of sulfite reductase activity.

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Cloning and Sequencing of Sulfite Reductase Subunit Gene from Saccharomyces cerevisiae

Cited by 3 publications

References 14 publications

Expression Profiles of Transcripts from 126 Open Reading Frames in the Entire Chromosome VI ofSaccharomyces cerevisiae by Systematic Northern Analyses

Expression Profiles of Transcripts from 126 Open Reading Frames in the Entire Chromosome VI ofSaccharomyces cerevisiae by Systematic Northern Analyses

Fifteen open reading frames in a 30·8 kb region of the right arm of chromosome VI fromSaccharomyces cerevisiae

Antifungal azoxybacilin exhibits activity by inhibiting gene expression of sulfite reductase

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