Cloning and sequence of partial cDNAs encoding the human type V and VI adenylyl cyclases and subsequent RNA-quantification in various tissues

Raimundo, Sebastian; Giray, Jeanette; Volff, Jean‐Nicolas; Schwab, Matthias; Altenbuchner, Joe; Ratge, D.; Wisser, H.

doi:10.1016/s0009-8981(99)00067-4

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…Quantitative data about mRNA expression levels of AC5 and AC6 in human hearts are conflicting [43], [44]. In adult murine cardiomyocytes, qRT-PCR readily detected mRNA expression for AC4, 5, 6 and 9, a weak expression for AC7 and no expression for AC1, 2, 3 and 8 [45].…”

Section: Resultsmentioning

confidence: 99%

Similarly Potent Inhibition of Adenylyl Cyclase by P-Site Inhibitors in Hearts from Wild Type and AC5 Knockout Mice

et al. 2013

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Adenylyl cyclase type 5 (AC5) was described as major cardiac AC isoform. The knockout of AC5 (AC5KO) exerted cardioprotective effects in heart failure. Our study explored the impact of AC5KO on mouse heart AC activities and evaluated putative AC5-selective inhibitors. In cardiac membranes from AC5KO mice, basal AC activity was decreased, while AC stimulation was intact. The putative AC5-selective P-site inhibitors SQ22,536 [9-(tetra-hydro-2-furanyl)-9H-purin-6-amine], vidarabine (9-β-D-arabinosyladenine) and NKY80 [2-amino-7-(2-furanyl)-7,8-dihydro-5(6H)-quinazolinone] inhibited recombinant AC5 more potently than AC2 and AC1, but selectivity was only modest (∼4-40-fold). These compounds inhibited cardiac AC from WT and AC5KO mice with similar potencies. In conclusion, AC regulation in AC5KO hearts was unimpaired, questioning the supposed dominant role of AC5 in the heart. Moreover, the AC inhibitors SQ22,536, NKY80 and vidarabine lack adequate selectivity for AC5 and, therefore, do not present suitable tools to study AC5-specific functions.

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Section: Resultsmentioning

confidence: 99%

Similarly Potent Inhibition of Adenylyl Cyclase by P-Site Inhibitors in Hearts from Wild Type and AC5 Knockout Mice

et al. 2013

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“…A possible function of these proteins is the physiological control of the digestive tube. Ci-ADCY5/6 is orthologous to human ADCY5 and ADCY6, both of which are expressed in the gut (Raimundo et al, 1999). It is known that the functioning of ADCY6 is inhibited by Ca 2+ (Stevens et al, 1995).…”

Section: Characteristics Of the Genes Expressed In The Digestive Tubementioning

confidence: 99%

Establishment of Enhancer Detection Lines Expressing GFP in the Gut of the Ascidian Ciona intestinalis

Yoshida

Sasakura

2012

Zoological Science

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The gut is a tubular, endodermal organ for digesting food and absorbing nutrients. In this study, we characterized eight enhancer detection lines that express green fluorescent protein (GFP) in the whole or part of the digestive tube of the ascidian Ciona intestinalis. Three enhancer detection lines for the pyloric gland, a structure associated with the digestive tube, were also analyzed. These lines are valuable markers for analyzing the mechanisms of development of the gut. Based on the GFP expression of the enhancer detection lines together with morphological characteristics, the digestive tube of Ciona can be subdivided into at least 10 compartments in which different genetic cascades operate. Causal insertion sites of the enhancer detection lines were identified, and the expression pattern of the genes near the insertion sites were characterized by means of whole-mount in situ hybridization. We have characterized four and two genes that were specifically or strongly expressed in the digestive tube and pyloric gland, respectively. The present data provide the basic information and useful resources for studying gut formation in Ciona.

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“…A HotStart PCR protocol was used to amplify adenylyl cyclase III. Additional primers were designed to amplify adenylyl cyclases V and VI using a partial sequence for Homo sapiens cardiac adenylyl cyclase types V and VI (Raimundo et al, 1999). For adenylyl cyclase V: forward primer of 20 bases representing nucleotides 41-60 and a reverse primer of 20 bases complementary to nucleotides 231-212.…”

Section: Rt-pcr Amplificationmentioning

confidence: 99%

Characterization of adenylyl cyclases in cultured human granulosa cells

Asboth¹

2001

Reproduction

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Cloning and sequence of partial cDNAs encoding the human type V and VI adenylyl cyclases and subsequent RNA-quantification in various tissues

Cited by 8 publications

References 11 publications

Similarly Potent Inhibition of Adenylyl Cyclase by P-Site Inhibitors in Hearts from Wild Type and AC5 Knockout Mice

Similarly Potent Inhibition of Adenylyl Cyclase by P-Site Inhibitors in Hearts from Wild Type and AC5 Knockout Mice

Establishment of Enhancer Detection Lines Expressing GFP in the Gut of the Ascidian Ciona intestinalis

Characterization of adenylyl cyclases in cultured human granulosa cells

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