Cloning and sequence of a secretory protein induced by growth factors in mouse fibroblasts

Purification and Characterization of Novel Heparin-binding Growth Factors in Uterine Secretory Fluids

“…Native hCTGF, partially purified on a PDGF immunoaffinity column, was reported to stimulate DNA synthesis in NIH 3T3 cells with an ED 50 of ϳ10 ng/ml (28). A similar ED 50 was shown for the (29). e From cDNA analysis in this study.…”

“…Also, by analogy with the genes for hCTGF, fisp-12 and nov, there is no intron/exon boundary that could directly give rise to the N terminus of the truncated pCTGF proteins (29,41,42), suggesting that alternative splicing alone is unlikely to be responsible for their production. Although the mechanism is as yet undefined, 10 -12-kDa forms of immunoreactive CTGF are also present in conditioned medium from cultured mouse or human fibroblasts, 2 suggesting that production of low mass CTGF is common to several species and biological systems.…”

“…Although CTGF mitogenic and chemotactic activities were reported to correlate with the presence of 36 -39-kDa proteins in human endothelial cell conditioned medium (28), these proteins were not individually isolated, tested for biological activity, or structurally characterized. Also, while the mouse CTGF gene product has been characterized as a nonglycosylated 35-kDa protein that is rapidly produced in and secreted by NIH 3T3 cells after serum stimulation (29), its intrinsic growth factor activity has not been reported. The susceptibility of CTGF to acid inactivation suggests that 10-kDa HBGF-0.8 proteins are likely to be more potent in their native state than after purification (i.e.…”

Human CYR61-mediated enhancement of bFGF-induced DNA synthesis in human umbilical vein endothelial cells

“…These secreted, cysteine-rich proteins have been named members of the`CCN family' (for Cyr61/Cef10, CTGF/Fisp12, and Nov; Bork, 1993) and include at least three distinct members: cyr61/cef10 (Simmons et al, 1989;O'Brien et al, 1990), CTGF/®sp12 (Bradham et al, 1991;Ryseck et al, 1991) and nov Joliot et al, 1992). Members of this protein family share a high level of sequence homology, including complete conservation of 38 cysteine residues in their secreted portion.…”

“…Both cyr61 (O'Brien et al, 1990;Nathans, 1985, 1987) and ®sp12 (Ryseck et al, 1991) were identi®ed as immediate-early genes, whose transcription is rapidly induced by serum growth factors without requiring de novo protein synthesis (Williams et al, 1992;Herschmann, 1991;Lau and Nathans, 1991). cef10, the chicken ortholog of cyr61, was also cloned as a growth factor-and v-src-inducible immediate-early gene (Simmons et al, 1989).…”