Cloning and sequence analysis of the <i>TRP1</i> gene encoding the phosphoribosyl anthranilate isomerase from <i>Pichia anomala</i> (strain K)

Friel, D.; Vandenbol, Micheline; Jijakli, M. Haïssam

doi:10.1002/yea.1033

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…This includes genes that can complement auxotrophic mutations, such as URA3 (J.A. Perez et al , unpublished: EMBL accession number Y09221), (Ogata et al , 1992), LEU2 (De la Rosa et al , 2001) and TRP1 (Friel et al , 2003), genes involved in the nitrate assimilation (Garcia‐Lugo et al , 2000), an invertase gene (Perez et al , 1996, 2001), SEC61 , which is involved in the secretory pathway (Ruiz et al , 2001, 2003), the gene for the alternative oxidase (Sakajo et al , 1999), and the PaEXG2 gene, encoding an exoglucanase that was suggested to be involved in the antifungal activity of P. anomala (Grevesse et al , 2003).…”

Section: Geneticsmentioning

confidence: 99%

Biotechnology, physiology and genetics of the yeastPichia anomala

Passoth

Fredlund

Druvefors

et al. 2006

FEMS Yeast Research

150

106

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The ascomycetous yeast Pichia anomala is frequently associated with food and feed products, either as a production organism or as a spoilage yeast. It belongs to the nonSaccharomyces wine yeasts and contributes to the wine aroma by the production of volatile compounds. The ability to grow in preserved food and feed environments is due to its capacity to grow under low pH, high osmotic pressure and low oxygen tension. A new application of P. anomala is its use as a biocontrol agent, which is based on the potential to inhibit a variety of moulds in different environments. Although classified as a biosafety class-1 organism, cases of P. anomala infections have been reported in immunocompromised patients. On the other hand, P. anomala killer toxins have a potential as antimicrobial agents. The yeast can use a broad range of nitrogen and phosphor sources, which makes it a potential agent to decrease environmental pollution by organic residues from agriculture. However, present knowledge of the physiological basis of its performance is limited. Recently, the first studies have been published dealing with the global regulation of the metabolism of P. anomala under different conditions of oxygenation.

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Section: Geneticsmentioning

confidence: 99%

Biotechnology, physiology and genetics of the yeastPichia anomala

Passoth

Fredlund

Druvefors

et al. 2006

FEMS Yeast Research

150

106

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“…S. fibuligera Trp1 protein shares the highest sequence identity (43.5%) with that of Komagataella pastoris, followed by those of Candida albicans (39.5%), C. orthopsilosis (39.1%), and Wickerhamomyces anomalus (38.4%). The alignment also confirmed several motifs highly conserved among fungal Trp1s: VGVFRNOS at amino acid positions 99-105, DFVQLHG at 120-126, and ILAGGLT at 200-206 (S. fibuligera numbering) [3,8,22]. Analysis by the C-DART program confirmed that S. fibuligera Trp1 shares a conserved structure with the PRAI family [9].…”

mentioning

confidence: 54%

“…Sequence alignment with other Trp1 proteins (Fig. 1) indicated that S. fibuligera Trp1 has a typical primary structure; the previously reported Trp1 domains [8,20] are highly conserved in S. fibuligera Tp1. S. fibuligera Trp1 protein shares the highest sequence identity (43.5%) with that of Komagataella pastoris, followed by those of Candida albicans (39.5%), C. orthopsilosis (39.1%), and Wickerhamomyces anomalus (38.4%).…”

mentioning

confidence: 58%

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Isolation of the Phosphoribosyl Anthranilate Isomerase Gene (TRP1) from Starch-Utilizing Yeast Saccharomycopsis fibuligera

2015

Journal of Microbiology and Biotechnology

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The nucleotide sequence of the TRP1 gene encoding phosphoribosyl anthranilate isomerase in yeast Saccharomycopsis fibuligera was determined by degenerate polymerase chain reaction and genome walking. Sequence analysis revealed the presence of an uninterrupted open-reading frame of 759 bp, including the stop codon, encoding a 252 amino acid residue. The deduced amino acid sequence of Trp1 in S. fibuligera was 43.5% homologous to that of Komagataella pastoris. The cloned TRP1 gene (SfTRP1) complemented the trp1 mutation in Saccharomyces cerevisiae, suggesting that it encodes a functional TRP1 in S. fibuligera. A new auxotrophic marker to engineer starch-degrading yeast S. fibuligera is now available. The GenBank Accession No. for SfTRP1 is KR078268.

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“…The Saccharomyces cerevisiae strain used was FY1679-18b, MATa ura3-52 leu2D1 trp1D63 his3D200 (Friel et al 2003). Yeasts were transformed using the method LiAc method of Ito et al (1983).…”

Section: Strains Dna Transformation and B-galactosidase Assaysmentioning

confidence: 99%

An mRNA sequence derived from the yeast EST3 gene stimulates programmed +1 translational frameshifting

Taliaferro¹,

Farabaugh²

2007

RNA

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Programmed translational frameshift sites are sequences in mRNAs that promote frequent stochastic changes in translational reading frame allowing expression of alternative forms of protein products. The EST3 gene of Saccharomyces cerevisiae, encoding a subunit of telomerase, uses a programmed +1 frameshift site in its expression. We show that the site is complex, consisting of a heptameric sequence at which the frameshift occurs and a downstream 27-nucleotide stimulator sequence that increases frameshifting eightfold. The stimulator appears to be modular, composed of at least three separable domains. It increases frameshifting only when ribosomes pause at the frameshift site because of a limiting supply of a cognate aminoacyltRNA and not when pausing occurs at a nonsense codon. These data suggest that the EST3 stimulator may modulate access by aminoacyl-tRNAs to the ribosomal A site by interacting with several targets in a ribosome paused during elongation.

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Cloning and sequence analysis of the TRP1 gene encoding the phosphoribosyl anthranilate isomerase from Pichia anomala (strain K)

Abstract: Pichia anomala (strain K) is an efficient biocontrol agent against post-harvest diseases affecting apples. To study the role of strain K genes in biocontrol activity, it is useful to identify selectable markers on which to base a gene disruption strategy. The Pichia anomala TRP1 gene (

Cited by 9 publications

References 16 publications

Biotechnology, physiology and genetics of the yeastPichia anomala

Biotechnology, physiology and genetics of the yeastPichia anomala

Isolation of the Phosphoribosyl Anthranilate Isomerase Gene (TRP1) from Starch-Utilizing Yeast Saccharomycopsis fibuligera

An mRNA sequence derived from the yeast EST3 gene stimulates programmed +1 translational frameshifting

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