Cloning and sequence analysis of a cDNA encoding porcine mitochondrial aspartate aminotransferase precursor.

Abstract

The primary structure of pig mitochondrial aspartate aminotransferase (mAspATase; L-aspartate:2-oxoglutarate aminotransferase, EC 2.6

Synthesis and Assembly of Mitochondrial Proteins

“…Proteins: (1) 70 kDa protein (yeast), Hase et al (1983); (2) cytochrome c peroxidase (yeast), Kaput et al (1982), Reid et al (1982); (3) cytochrome b 2 (yeast), Guiard (1985), Gasser et al (1982b); (4) Rieske Fe/S of bc, complex (N. crassa), Harnisch et al (1985), Hartl et al (1986); (5) cytochrome d (yeast), Sadler et al (1984), Gasser et al (1982b); (6) cytochrome oxidase V (yeast), Koerner et aJ. (1985); (7) cytochrome oxidase V (N. crassa), Sachs et al (1986); (8) cytochrome oxidase IV (bovine), Lomax et al (1984); (9) cytochrome P-450 (SCC) (bovine), Morohashi et al (1984); (10) cytochrome oxidase VI (yeast), Wright et al (1984) ; (11) ATPase IX (N. crassa), Viebrock et al (1982), Schmidt et al (1984); (12) ATPase IX-Pl (bovine), Gay and Walker (1985); (13) ATPase IX-P2 (bovine), Gay and Walker (1985); (14) cytochrome oxidase IV (yeast), Maarse et al (1984); (15) citrate synthase (yeast), Suissa et al (1984); (16) Ornithine aminotransferase (rat), Mueckler and Pitot (1985), Simmaco et al (1986); (17) aspartate aminotransferase (chicken), Jaussi et al (1985) ; (18) aspartate aminotransferase (porcine), Joh et al (1985); (19) Mn-superoxide dismutase (yeast), Marres et al (1985); (20) ATPase F, 5 subunit (N. crassa), Kruse and Sebald (1984); (21) 5-aminolevulinate synthase (yeast), Keng et al (1986), Urban-Grimal et al (1986); (22) alcohol dehydrogenase iso-III (yeast), Young and Pilgrim (1985); (23) Ornithine carbamoyltransferase (human), Horwich et al (1984); (24) Ornithine carbamoyltransferase (rat), Mclntyre et al (1...…”

Structure of the genes of two homologous intracellularly heterotopic isoenzymes

“…198 Biol. 200,[13][14][15][16][17][18][19][20][21][22]] reveals closely similar structures: in the gene of both the mitochondrial and the cytosolic isoenzyme all but one intron positions are conserved in the two species and five introns out of nine are placed at the same positions in all four genes indicating that the introns were in place before the genes of the two isoenzymes diverged.…”

“…The complete amino acid sequences of the two isoenzymes from pig [2-41, chicken [5, 61 and horse [7] have been determined. The nucleotide sequences of the cDNAs for both isoenzymes are known for chicken [8, 91, mouse [lo], rat [I 1, 121 and pig [13,14]. Mitochondria1 AspAT is one ofthe typical nuclear-encoded mitochondrial proteins that are synthesized as larger precursors on free ribosomes in the cytosol [15], transported through the mitochondrial envelope, and processed to the mature form in the mitochondrial matrix (for reviews, see [16,171).…”

Aspartate Aminotransferase in Effective and Ineffective Alfalfa Nodules

“…In animals, cytosolic and mitochondrial forms of AAT have been extensively characterized, and the genes encoding these proteins have been isolated (e.g. 14,22). Plant AAT genes have not been isolated.…”