Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China

Yang, Huey‐Lang; Makeyev, Eugene V.; Kang, Zi-Xuan; S, Ji; Bamford, Dennis H.; Dijk, Alberdina A. van

doi:10.1016/j.virusres.2004.05.011

Cited by 47 publications

(34 citation statements)

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“…The genome of J19 is AUrich (66.49 %), with a base composition of A (36.45 %), G (17.83 %), T (30.04 %) and C (15.68 %), and 66.1 % Table 1 shows non-protein-coding features of these 11 gene segments of NADRV J19. From these data, a terminal GGsequence, common to all rotaviruses, was located at the 59-end of all the cloned genes, while at the 39-end of the genes -ATACCC sequence was found in 10 cloned genes (1-7 and 9-11), -ACACCC sequence was found only in the NSP3 gene, which is similar to that of NADRV and B219, but different from those of groups A, B and C rotaviruses (Yang et al, 2004a;Alam et al, 2007). Analysis of the 11 messenger sense RNA fragments with an RNA secondary structure prediction program (GeneBee) demonstrated that all of them could base-pair with themselves for nearly the entire length of the RNA and their 59 and 39 termini could form panhandle structures (Brodsky et al, 1992(Brodsky et al, , 1995.…”

Section: Complete J19 Genomic Cloning and Sequencingmentioning

confidence: 80%

“…The same rotavirus was implicated in gastroenteritis outbreaks in Beijing city in 1994 and in Shijiazhuang city in 1997. Partial genes of NADRV were cloned and sequenced from stool samples of patients and primary results showed that the novel rotavirus did not belong to known groups A, B or C rotaviruses (Yang et al, 1998(Yang et al, , 2004a. Later, an unusual human rotavirus strain, B219, was detected in a stool specimen from a 65-year-old patient with diarrhoea in Bangladesh during April 2002, which is genetically closely related to the NADRV strain (Alam et al, 2007).…”

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confidence: 99%

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Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

Jiang

Tang

et al. 2008

Journal of General Virology

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The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method. The complete genome is 17 961 bp and is . Northern blot analysis and genomic sequence analysis indicated that segments 1-11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the J19 proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus strain with great significance to the evolution and origin of group B rotaviruses. INTRODUCTIONRotaviruses are important aetiological agents of disease in humans and animals. The viral genome is composed of 11 segments of double-stranded (ds) RNA that encode structural and non-structural proteins. The group A rotavirus strain SA11 genome, sequenced in 1990, is AUrich and has 18 555 bp (Both et al., 1984;Estes et al., 1984;Mitchell & Both, 1990). Its RNA segments encode six structural proteins (VP1, VP2, VP3, VP4, VP6 and VP7) and five non-structural proteins (NSP1, NSP2, NSP3, NSP4 and NSP5) (Estes, 2001). VP6 is the group antigen determinant, making up approximately 50 % of the viral protein (Estes et al., 1984;Estes & Cohen, 1989). The antigenic properties of VP6 are used to define seven antigenically different rotavirus groups, named groups A-G. Groups A, B and C rotaviruses infect humans, while other groups have been found only in animal species (Saif & Jiang, 1994). Group A rotaviruses cause severe diarrhoea in infants and young children, but they can also infect adults. Group C rotavirus infection occurs both in children and in adults, usually in sporadic cases or clustered outbreaks (Rodger et al., 1982;Caul et al., 1990;Jiang et al.,1995; Kuzuya et al., 1996; Nilsson et al., 2000;Adah et al., 2002;Chen et al., 2002;Ji et al., 2002;Schnagl et al., 2004).In China, large waterborne epidemics caused by the human group B rotavirus strain adult diarrhoea rotavirus (ADRV) infected thousands of people aged between 10 and 40 years of age in the 1980s (Hung et al., 1983(Hung et al., , 1984Chen et al., 1985Chen et al., , 1990 et al., 1987). The same rotavirus was implicated in gastroenteritis outbreaks in Beijing city in 1994 and in Shijiazhuang city in 1997. Partial genes of NADRV were clon...

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Section: Complete J19 Genomic Cloning and Sequencingmentioning

confidence: 80%

mentioning

confidence: 99%

Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

Jiang

Tang

et al. 2008

Journal of General Virology

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“…Interestingly, the (+)RNAs of all groups of rotavirus examined to date (groups A, B, and C) initiate with the dinucleotide GG, indicating that the 5¢ recognition signal is highly conserved within this genera of viruses (Yang et al 2004). In contrast, the C3 residue is not conserved among all virus groups and is not required for activity of the 5¢ recognition signal.…”

Section: Discussionmentioning

confidence: 99%

A base-specific recognition signal in the 5′ consensus sequence of rotavirus plus-strand RNAs promotes replication of the double-stranded RNA genome segments

Tortorici

Shapiro²,

Patton³

2005

RNA

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Replication of the segmented double-stranded (ds)RNA genome of rotavirus requires the viral RNA-dependent RNA polymerase (RdRP) to use 11 different (+)RNAs as templates for (À) strand synthesis. Complementary sequences proximal to the 5¢ and 3¢ termini are predicted to direct cyclization of the (+)RNAs by forming panhandle structures from which short highly conserved terminal sequences protrude as single-stranded tails. Cell-free replication assays indicate that such structural organization of the 5¢-and 3¢-ends is required for efficient dsRNA synthesis. Multiple specifically recognized elements exist at the 3¢-end that promote dsRNA synthesis including RdRP-recruitment signals and a (À) strand initiation sequence. In contrast to the 3¢-end, the role of the 5¢-end has been less well defined. In this study, we determined that the 5¢-end contains a base-specific recognition signal that plays an important role in the assembly of the RdRP and cofactors into a stable initiation complex for (À) strand synthesis. The 5¢ recognition signal is associated with the G2 residue of the 5¢-consensus sequence, a residue that shows absolute conservation among all rotavirus groups (A, B, and C) examined to date. From our results, we suggest that rotavirus (+)RNA cyclization, although likely initiated by 5¢-3¢ nucleotide complementarity, may be stabilized by RdRP-dependent bridging. Given that synthesis of the (À) strand on the (+)RNA template will disrupt 5¢-3¢ nucleotide interactions, RdRP-dependent bridging may be the sole mechanism by which the dsRNA product can be held in the necessary cyclized conformation required for efficient multiple rounds of transcription.

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“…Among RVs, there are eight major groups (A to H) identified with RVA being the most predominant across the world (Yang et al 2004;Matthijnssens et al 2011). Classification of AvRVs was initially ascertained by cross-immunofluorescence studies or polyacrylamide gel electrophoresis (PAGE) analysis of ds RNA segments (Guy 1998).…”

Section: Virus Classification and Genome Organizationmentioning

confidence: 99%

Avian rotavirus enteritis – an updated review

Dhama

Saminathan

Karthik

et al. 2015

Veterinary Quarterly

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Cloning and sequence analysis of dsRNA segments 5, 6 and 7 of a novel non-group A, B, C adult rotavirus that caused an outbreak of gastroenteritis in China

Cited by 47 publications

References 50 publications

Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses

A base-specific recognition signal in the 5′ consensus sequence of rotavirus plus-strand RNAs promotes replication of the double-stranded RNA genome segments

Avian rotavirus enteritis – an updated review

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