Cloning and salt tolerance analysis of the PnHB7 transcription factor in Populus nigra L

Yu, Xiaoyue; Du, Jiujun; Dong, Yan; Lu, Bin

doi:10.1016/j.indcrop.2020.112943

Cited by 8 publications

(5 citation statements)

References 49 publications

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“…ZF_HD family consist of 22 transcription factors that belong to Gossypium arboreum species that were divided in three main subfamilies A, B and C. Family B is further subdivided into B1, and B2 subfamilies and GaZnF transcription factor belongs to transcription factors A-19854 and A-27654 that are known as zinc finger homeodomain proteins and thought to be involved in the formation of homo and heterodimers and may form a zinc finger 31 . In the present study, the subcellular localization of GaZnF in the nucleus showed consistency with the characteristics of TFs 11 . The integration of GaZnF in genomic DNA of transgenic plants revealed 531 bp fragment.…”

Section: Discussionsupporting

confidence: 84%

“…The protein motif consists of cysteine (C) or histidine (H) residues coordinated with several zinc ions. Different unique and important TFs have been discovered at the molecular level that will increase the plant adaptation to drought stress 11 by regulating the expression of stress-related genes 12 , 13 . A stress tolerant Zinc finger transcription factor gene ( GaZnF ) was previously isolated from G. arboreum 10 .…”

Section: Introductionmentioning

confidence: 99%

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Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum

Fatima

Hassan

Azam

et al. 2023

Sci Rep

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Drought stress is the major limiting factor in plant growth and production. Cotton is a significant crop as textile fiber and oilseed, but its production is generally affected by drought stress, mainly in dry regions. This study aimed to investigate the expression of Zinc finger transcription factor’s gene (GaZnF) to enhance the drought tolerance in Gossypium hirsutum. Sequence features of the GaZnF protein were recognized through different bioinformatics tools like multiple sequence alignment analysis, phylogenetic tree for evolutionary relationships, Protein motifs, a transmembrane domain, secondary structure and physio-chemical properties indicating that GaZnF is a stable protein. CIM-482, a local Gossypium hirsutum variety was transformed with GaZnF through Agrobacterium-mediated transformation method with 2.57% transformation efficiency. The integration of GaZnF was confirmed through Southern blot showing 531 bp, and Western blot indicated a 95 kDa transgene-GUS fusion band in transgenic plants. The normalized real-time expression analysis revealed the highest relative fold spatial expression of cDNA of GaZnF within leaf tissues at vegetative and flowering stages under drought stress. Morphological, physiological and biochemical parameters of transgenic cotton plants at 05- and 10-day drought stress was higher than those of non-transgenic control plants. The values of fresh and dry biomass, chlorophyll content, photosynthesis, transpiration rate, and stomatal conductance reduced in GaZnF transgenic cotton plants at 05- and 10-day drought stress, but their values were less low in transgenic plants than those of non-transgenic control plants. These findings showed that GaZnF gene expression in transgenic plants could be a valuable source for the development of drought-tolerant homozygous lines through breeding.

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Section: Discussionsupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum

Fatima

Hassan

Azam

et al. 2023

Sci Rep

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“…The evolution of genes from different species was analyzed using the neighbor-joining method in MEGA ver. 7.0 (https://www.megasoftware.net) [25], and a phylogenetic tree was constructed using the results (Fig. 2b).…”

Section: Bioinformatics Analysis Of Pewrky31mentioning

confidence: 99%

“…The latest release of the PlantTFDB (http://planttfdb.cbi.pku.edu.cn/)transcription factor database contains a total of 185 Populus tomentosa WRKYs. Over a dozen related transcription factors have been isolated, their functions have been verified [25], and the WRKY family genes in many poplar trees have been shown to respond to abiotic stress and play important roles in salt resistance [26][27][28]. Preliminary laboratory research revealed that PeWRKY31 of Populus × euramericana responded to biological stress, but the underlying mechanism was not clear.…”

Section: Introductionmentioning

confidence: 99%

Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana

Pan

Dong

et al. 2021

BMC Plant Biol

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Background As important forest tree species, biological stress and soil salinization are important factors that restrict the growth of Populus × euramericana. WRKYs are important transcription factors in plants that can regulate plant responses to biotic and abiotic stresses. In this study, PeWRKY31 was isolated from Populus × euramericana, and its bioinformation, salt resistance and insect resistance were analyzed. This study aims to provide guidance for producing salt-resistant and insect-resistant poplars. Results PeWRKY31 has a predicted open reading frame (ORF) of 1842 bp that encodes 613 amino acids. The predicted protein is the unstable, acidic, and hydrophilic protein with a molecular weight of 66.34 kDa, and it has numerous potential phosphorylation sites, chiefly on serines and threonines. PeWRKY31 is a zinc-finger C2H2 type-II WRKY TF that is closely related to WRKY TFs of Populus tomentosa, and localizes to the nucleus. A PeWRKY31 overexpression vector was constructed and transformed into Nicotiana tabacum L. Overexpression of PeWRKY31 improved the salt tolerance and insect resistance of the transgenic tobacco. Transcriptome sequencing and KEGG enrichment analysis showed the elevated expression of genes related to glutathione metabolism, plant hormone signal transduction, and MAPK signaling pathways, the functions of which were important in plant salt tolerance and insect resistance in the overexpressing tobacco line. Conclusions PeWRKY31 was isolated from Populus × euramericana. Overexpression of PeWRKY31 improved the resistance of transgenic plant to salt stress and pest stress. The study provides references for the generation of stress-resistant lines with potentially great economic benefit.

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“…Populus tomentosa generally grows upright and has wood that is compact and delicate with very high fiber content, making it particularly useful for plate making, paper making, and other industrial applications. Previous studies have shown that modifying BR biosynthesis genes such as DWF4 and CYP85 enhances growth, biomass accumulation, and timber quality [ 26 , 27 , 28 ]. OsCYP85A1 / OsBRD1 is a key gene for CS synthesis in rice, which plays an important role in its rapid growth.…”

Section: Introductionmentioning

confidence: 99%

The Overexpression of Oryza sativa L. CYP85A1 Promotes Growth and Biomass Production in Transgenic Trees

Chen

Tian

et al. 2023

IJMS

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Brassinosteroids (BRs) are important hormones that play crucial roles in plant growth, reproduction, and responses to abiotic and biotic stresses. CYP85A1 is a castasterone (CS) synthase that catalyzes C-6 oxidation of 6-deoxocastasterone (6-deoxoCS) to CS, after which CS is converted into brassinolide (BL) in a reaction catalyzed by CYP85A2. Here, we report the functional characteristics of rice (Oryza sativa L.) OsCYP85A1. Constitutive expression of OsCYP85A1 driven by the cauliflower mosaic virus 35S promoter increased endogenous BR levels and significantly promoted growth and biomass production in three groups of transgenic Populus tomentosa lines. The plant height and stem diameter of the transgenic poplar plants were increased by 17.6% and 33.6%, respectively, in comparison with control plants. Simultaneously, we showed that expression of OsCYP85A1 enhanced xylem formation in transgenic poplar without affecting cell wall thickness or the composition of cellulose. Our findings suggest that OsCYP85A1 represents a potential target candidate gene for engineering fast-growing trees with improved wood production.

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Cloning and salt tolerance analysis of the PnHB7 transcription factor in Populus nigra L

Cited by 8 publications

References 49 publications

Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum

Transformation and expressional studies of GaZnF gene to improve drought tolerance in Gossypium hirsutum

Cloning and overexpression of PeWRKY31 from Populus × euramericana enhances salt and biological tolerance in transgenic Nicotiana

The Overexpression of Oryza sativa L. CYP85A1 Promotes Growth and Biomass Production in Transgenic Trees

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