Cloning and polymorphisms of the 3′ untranslated region of malic enzyme gene in Chinese red cattle

Zhou, Guoli; Zhang, G.L.; Cao, Yang; Jin, Haiguo

doi:10.1016/j.meatsci.2011.03.023

Cited by 9 publications

(12 citation statements)

References 19 publications

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“…Finally, one SNP in the 3′UTR (NW_0014955 44:g.1536016T>C) showed a significant association with cooking loss, pH measured 24 h postmortem and eye muscle area in the Chinese red cattle. The animals with the CC genotype had a lower cooking loss and greater eye muscle area than those with the TT or CT genotypes (Zhou et al 2011).…”

Section: Association Between Snps and Production Traitsmentioning

confidence: 87%

“…Association of the ME1 gene polymorphism with production traits was also studied in cattle (Zhou et al 2011;Gill et al 2012). It was claimed that a non-synonymous SNP in exon 8, causing valine-to-isoleucine substitution, is strongly associated with sirloin weightand fat-related traits in commercial cattle crossed with Aberdeen Angus sires.…”

Section: Association Between Snps and Production Traitsmentioning

confidence: 99%

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Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Bartz

Kociucka

Mańkowska

et al. 2013

J Animal Breeding Genetics

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Pork quality depends on multiple factors, including fatty acid composition in muscle and fat tissues. The ME1 gene is a strong candidate for fat accumulation, as it encodes the malic enzyme, which is required for fatty acid synthesis. We identified seven new polymorphisms in 3'UTR of the ME1 gene and moreover confirmed the presence of 4 polymorphisms detected previously. Interestingly, the studied Duroc pigs were monomorphic at all these polymorphic sites, while in 3 other breeds (Pietrain, Polish Landrace and Polish Large White), the polymorphisms were unevenly distributed. One of the novel SNPs (c.*488A>G) was found in the Polish Large White and the Polish Landrace only, and the association studies revealed that it was significantly associated with backfat thickness and average daily weight gain in the Polish Landrace (N = 207) and the Polish Large White (N = 157). This SNP was differently associated with ME1 transcript level in muscle and backfat. The in silico analysis of another novel SNP (c.*548C>T) indicated that it is located within a binding sequence conserved among vertebrates for the miR-30 family in 3'UTR of the ME1. It was shown that in the longissimus muscle, but not in adipose tissue, CT gilts compared with CC ones had significantly lower levels of the ME1 transcript. This polymorphism, however, was not associated with production traits. Additionally, we observed that transcript level of the ME1 was significantly higher in subcutaneous fat than in the longissimus muscle, as well as both investigated tissues of the Polish Landrace when compared to the other breeds. However, no association was found between this polymorphism and fatty acid profiles. We conclude that the ME1 gene polymorphism (c.*488A>G) is a potential marker for porcine backfat thickness.

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Section: Association Between Snps and Production Traitsmentioning

confidence: 87%

Section: Association Between Snps and Production Traitsmentioning

confidence: 99%

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Bartz

Kociucka

Mańkowska

et al. 2013

J Animal Breeding Genetics

View full text Add to dashboard Cite

show abstract

“…Polymorphisms of ME1 gene have been shown to influence meat quality and carcass traits in beef cattle (Zhou et al 2011;Gill et al 2012). Gill et al (2012) revealed an association between the c.1001+66C>T SNP in ME1 gene and pH 24h in Aberdeen Angus beef cattle population.…”

Section: Discussionmentioning

confidence: 98%

“…A SNP was detected in 3' UTR of ME1 gene in Chinese Red cattle and this mutation creates a DraI restriction site. The ME1-DraI polymorphism was shown to be associated with meat quality and carcass traits in Chinese red cattle (Zhou et al 2011). Gill et al (2012) have demonstrated that the c.903 + 11A>T and c.1001 + 66C>T SNPs in bovine ME1 gene are associated with taste panel assessed juiciness and pH at 24 h, respectively.…”

Section: Introductionmentioning

confidence: 99%

Identification of SNPs in ME1 gene and association analysis with meat quality traits in Chinese Red cattle

Zhou¹,

Cao²,

Jin³

2014

CZECH J ANIM SCI

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ABSTRACT:The objective of this study was to identify single nucleotide polymorphisms (SNPs) in coding regions of bovine ME1 gene and to evaluate if the polymorphisms are associated with meat quality traits in Chinese Red cattle. Four SNPs were identified: NW_001495544:g.1721768G>A in exon 2, g.1653796T>A in exon 4, g.1649532G>A in exon 5, and g.1546272T>C in exon 12 and they were genotyped by applying the PCR-RFLP method. Statistical analysis showed that two SNPs, g.1649532G>A and g.1546272T>C, were significantly associated with cooking loss and pH 24h (P < 0.05). But no statistically significant differences were observed in the g.1721768G>A and g.1653796A>T SNPs for meat quality traits tested in Chinese Red cattle. This suggests that ME1 gene is a candidate that may have effects on meat quality traits in cattle.

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“…For instance, Yie et al (2008) identified a single base pair mutation in the human leukocyte antigen-G 3'UTR, which was thought to be a fundamental mechanism for lowering the levels of placental human leukocyte antigen-G protein expression in patients with preeclampsia. Zhou et al (2011) identified an SNP in the malic enzyme 1 gene 3'UTR, and showed that it was correlated with ultrasound in Chinese red cattle.…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms in the SIRT5 gene and their association with body measurement and ultrasound traits in Qinchuan cattle

Gui¹,

Wang²,

Liu³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Silent information regulator 5 (SIRT5), a member of the Sirtuin family class III nicotinamide adenine dinucleotide-dependent protein deacetylases, plays an important role in metabolic and aging processes in mammals. We identified 4 single-nucleotide polymorphisms (SNPs) (G22010A, G22052A, G22119T, and G22245C) in the 3' untranslated regions of the SIRT5 gene from 572 Qinchuan cattle by sequencing and investigating their association with growth and ultrasound traits. The frequencies of genotype GG and allele G were high at the 4 SNPs. Based on the c 2 test, the genotypic distributions of the 4 SNPs were not in Hardy-Weinberg equilibrium (P < 0.05 or P < 0.01). Association analysis of individual SNPs and haplotype combinations revealed that the 4 loci were significantly associated with some body measurement and ultrasound traits in Qinchuan cattle, L.S. Gui et al. 3844©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 3843-3853 (2015) and the H 1 H 5 (AG-GA-GG-GG) diplotypes had better performance than other combinations in Qinchuan cattle. Our results demonstrate that SIRT5 may be a candidate for marker-assisted selection in future breeding programs for Qinchuan cattle.

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Cloning and polymorphisms of the 3′ untranslated region of malic enzyme gene in Chinese red cattle

Cited by 9 publications

References 19 publications

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Transcript level of the porcine ME1 gene is affected by SNP in its 3′UTR, which is also associated with subcutaneous fat thickness

Identification of SNPs in ME1 gene and association analysis with meat quality traits in Chinese Red cattle

Polymorphisms in the SIRT5 gene and their association with body measurement and ultrasound traits in Qinchuan cattle

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