Cloning and partial characterization of a Boophilus microplus (Acari: Ixodidae) glutathione S-transferase

Lima, Monica Fernandes Rosa de; Ferreira, Carlos Alexandre Sanchez; Freitas, Daniela Reis Joaquim de; Valenzuela, Jesús G.; Masuda, Aoi

doi:10.1016/s0965-1748(01)00157-6

Cited by 52 publications

(33 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other examples of housekeeping protein involve enzymes linked to detoxification (Table 1). Glutathione S-transferase (GST) is a protein that catalyzes the conjugation of glutathione with several xenobiotic and endogenous substances [164]. In this sense, GST seems to be closely associated with detoxification and acaricide resistance [165].…”

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Cattle Tick Rhipicephalus (Boophilus) microplus Saliva: A Comparison between Partially and Fully Engorged Females

et al. 2014

View full text Add to dashboard Cite

The cattle tick Rhipicephalus (Boophilus) microplus is one of the most harmful parasites affecting bovines. Similarly to other hematophagous ectoparasites, R. microplus saliva contains a collection of bioactive compounds that inhibit host defenses against tick feeding activity. Thus, the study of tick salivary components offers opportunities for the development of immunological based tick control methods and medicinal applications. So far, only a few proteins have been identified in cattle tick saliva. The aim of this work was to identify proteins present in R. microplus female tick saliva at different feeding stages. Proteomic analysis of R. microplus saliva allowed identifying peptides corresponding to 187 and 68 tick and bovine proteins, respectively. Our data confirm that (i) R. microplus saliva is complex, and (ii) that there are remarkable differences in saliva composition between partially engorged and fully engorged female ticks. R. microplus saliva is rich mainly in (i) hemelipoproteins and other transporter proteins, (ii) secreted cross-tick species conserved proteins, (iii) lipocalins, (iv) peptidase inhibitors, (v) antimicrobial peptides, (vii) glycine-rich proteins, (viii) housekeeping proteins and (ix) host proteins. This investigation represents the first proteomic study about R. microplus saliva, and reports the most comprehensive Ixodidae tick saliva proteome published to date. Our results improve the understanding of tick salivary modulators of host defense to tick feeding, and provide novel information on the tick-host relationship.

show abstract

Section: Resultsmentioning

confidence: 99%

Proteomic Analysis of Cattle Tick Rhipicephalus (Boophilus) microplus Saliva: A Comparison between Partially and Fully Engorged Females

et al. 2014

View full text Add to dashboard Cite

show abstract

“…GST expression has been previously induced upon blood feeding in R. microplus , and is postulated to be an adaptive response to reactive oxygen species created during the blood meal [17]. GSTs may also facilitate digestion of the meal by reducing proteins and lipids [18]. Additional unigenes were similar to other protein-reducing antioxidants including glutaredoxin (ContigA561), peroxiredoxin (MPOAE24TR), and several peroxinectins (ContigA694, MPOA737TR, MPOA895TR, MPOA475TR, MPOAC37TR and MPOAK85TR).…”

Section: Resultsmentioning

confidence: 99%

The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis

et al. 2013

View full text Add to dashboard Cite

BackgroundCattle babesiosis is a tick-borne disease of cattle with the most severe form of the disease caused by the apicomplexan, Babesia bovis. Babesiosis is transmitted to cattle through the bite of infected cattle ticks of the genus Rhipicephalus. The most prevalent species is Rhipicephalus (Boophilus) microplus, which is distributed throughout the tropical and subtropical countries of the world. The transmission of B. bovis is transovarian and a previous study of the R. microplus ovarian proteome identified several R. microplus proteins that were differentially expressed in response to infection. Through various approaches, we studied the reaction of the R. microplus ovarian transcriptome in response to infection by B. bovis.MethodsA group of ticks were allowed to feed on a B. bovis-infected splenectomized calf while a second group fed on an uninfected splenectomized control calf. RNA was purified from dissected adult female ovaries of both infected and uninfected ticks and a subtracted B. bovis-infected cDNA library was synthesized, subtracting with the uninfected ovarian RNA. Four thousand ESTs were sequenced from the ovary subtracted library and annotated.ResultsThe subtracted library dataset assembled into 727 unique contigs and 2,161 singletons for a total of 2,888 unigenes, Microarray experiments designed to detect B. bovis-induced gene expression changes indicated at least 15 transcripts were expressed at a higher level in ovaries from ticks feeding upon the B. bovis-infected calf as compared with ovaries from ticks feeding on an uninfected calf. We did not detect any transcripts from these microarray experiments that were expressed at a lower level in the infected ovaries compared with the uninfected ovaries. Using the technique called serial analysis of gene expression, 41 ovarian transcripts from infected ticks were differentially expressed when compared with transcripts of controls.ConclusionCollectively, our experimental approaches provide the first comprehensive profile of the R. microplus ovarian transcriptome responding to infection by B. bovis. This dataset should prove useful in molecular studies of host-pathogen interactions between this tick and its apicomplexan parasite.

show abstract

“…Hemelipoglycoproteins and cytochrome c are involved in the detoxification process or energy production of larval stages [48]. The major functions of GSTs include the detoxification of xenobiotics, digestive processes, and prostaglandin synthesis and they are also associated with a series of reactions essential to protect cell constituents from oxidative attack by oxygen and oxygen-associated free radicals [49]. However, further work is needed to understand the physiological role of GSTs in tick metabolism as well as to evaluate its function during tick-host interaction.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, it has been suggested that blood digestion is a source of oxidative stress for blood-feeding parasites, and several antioxidant defences and haeme detoxification mechanisms have been shown to contribute to the adaptation of these parasites to a hematophagous lifestyle [81]. In R. microplus , transcripts related to managing oxidative stress were identified (n = 2) including a transcript with significant similarity to a putative glutathione S-transferase [49]. …”

Section: Resultsmentioning

confidence: 99%

Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle

et al. 2010

View full text Add to dashboard Cite

BackgroundRhipicephalus (Boophilus) microplus is an obligate blood feeder which is host specific to cattle. Existing knowledge pertaining to the host or host breed effects on tick transcript expression profiles during the tick - host interaction is poor.ResultsGlobal analysis of gene expression changes in whole R. microplus ticks during larval, pre-attachment and early adult stages feeding on Bos indicus and Bos taurus cattle were compared using gene expression microarray analysis. Among the 13,601 R. microplus transcripts from BmiGI Version 2 we identified 297 high and 17 low expressed transcripts that were significantly differentially expressed between R. microplus feeding on tick resistant cattle [Bos indicus (Brahman)] compared to R. microplus feeding on tick susceptible cattle [Bos taurus (Holstein-Friesian)] (p ≤ 0.001). These include genes encoding enzymes involved in primary metabolism, and genes related to stress, defence, cell wall modification, cellular signaling, receptor, and cuticle formation. Microarrays were validated by qRT-PCR analysis of selected transcripts using three housekeeping genes as normalization controls.ConclusionThe analysis of all tick stages under survey suggested a coordinated regulation of defence proteins, proteases and protease inhibitors to achieve successful attachment and survival of R. microplus on different host breeds, particularly Bos indicus cattle. R. microplus ticks demonstrate different transcript expression patterns when they encounter tick resistant and susceptible breeds of cattle. In this study we provide the first transcriptome evidence demonstrating the influence of tick resistant and susceptible cattle breeds on transcript expression patterns and the molecular physiology of ticks during host attachment and feeding.The microarray data used in this analysis have been submitted to NCBI GEO database under accession number GSE20605 http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE20605.

show abstract

Cloning and partial characterization of a Boophilus microplus (Acari: Ixodidae) glutathione S-transferase

Cited by 52 publications

References 50 publications

Proteomic Analysis of Cattle Tick Rhipicephalus (Boophilus) microplus Saliva: A Comparison between Partially and Fully Engorged Females

Proteomic Analysis of Cattle Tick Rhipicephalus (Boophilus) microplus Saliva: A Comparison between Partially and Fully Engorged Females

The ovarian transcriptome of the cattle tick, Rhipicephalus (Boophilus) microplus, feeding upon a bovine host infected with Babesia bovis

Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle

Contact Info

Product

Resources

About