Cloning and Overexpression of the Xylitol Dehydrogenase Gene from <i>Bacillus pallidus</i> and Its Application to <scp>L</scp>-Xylulose Production

Takata, Goro; Poonperm, Wayoon; Morimoto, Kenji; Izumori, Ken

doi:10.1271/bbb.100144

Cited by 20 publications

(18 citation statements)

References 32 publications

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“…2). This result is consistent with a previous study of l-xylulose production using resting cells expressing xylitol dehydrogenase, where Takata et al achieved approximately 53% conversion with a substrate concentration of 2% (133 mM), but only achieved a 36% conversion rate with a substrate concentration of 5% [12]. To further improve the conversion of l-arabinitol to l-xylulose under conditions of high substrate concentration, we investigated the effect of pH on lxylulose yield.…”

Section: Resultssupporting

confidence: 90%

“…Additionally, l-xylulose produced by B. pallidus Y25 strain can be partially converted to l-xylose and larabinitol because of the intrinsic isomerase activity in B. pallidus [10]. Resting E. coli cells harboring the recombinant xylitol dehydrogenase gene of B. pallidus have been reported to produce l-xylulose from 2% xylitol, with a yield of approximately 53% [12].…”

Section: Resultsmentioning

confidence: 99%

“…701B [9], and Bacillus pallidus Y25 [10]. Furthermore, the genes encoding the xylitol dehydrogenase from P. ananatis ATCC 43,072 [11] and B. pallidus [12] have been cloned, and recombinant strains containing these genes have been used to produce l-xylulose. Highefficiency conversions of xylitol to l-xylulose (∼70% conversion) have previously been achieved by a recombinant Escherichia coli strain; however, this conversion was only achieved under low (<67 mM) xylitol conditions [13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Gao

Kim

Choi³

et al. 2015

Biochemical Engineering Journal

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Gao

Kim

Choi³

et al. 2015

Biochemical Engineering Journal

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“…In a recent study, the XDH from Bacillus pallidus has been overexpressed in Escherichia coli for L-xylulose production. Although the conversion rates were lower than when the B. pallidus strain was used, the advantage of E. coli is that the formation of side products is drastically reduced [87,105]. At higher temperatures, however, L-xylose started to accumulate instead of L-xylulose, perhaps due to the activity of endogenous D-arabinose isomerase in E. coli.…”

Section: Oxidoreductasesmentioning

confidence: 95%

“…Starting material for the synthesis of the nucleosides against HBV [69] Synthesis of L-ribofuranose derivatives [8] L-Xylulose Potential inhibitor of various glucosidases [56,61] Synthesis of L-xylose and L-lyxose [26] Indicator of hepatitis or liver cirrhosis [105] HBV Hepatitis B virus, HIV human immunodeficiency virus J Ind Microbiol Biotechnol (2012) 39:823-834 825 (Table 2). Isomerases, for example, are promiscuous biocatalysts that are active on a range of simple substrates, i.e., unsubstituted monosaccharides.…”

Section: Enzymes For Rare Sugar Productionmentioning

confidence: 99%

Enzymes for the biocatalytic production of rare sugars

Beerens

Desmet

Soetaert

2012

Journal of Industrial Microbiology and Biotechnology

168

109

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Carbohydrates are much more than just a source of energy as they also mediate a variety of recognition processes that are central to human health. As such, saccharides can be applied in the food and pharmaceutical industries to stimulate our immune system (e.g., prebiotics), to control diabetes (e.g., low-calorie sweeteners), or as building blocks for anticancer and antiviral drugs (e.g., L: -nucleosides). Unfortunately, only a small number of all possible monosaccharides are found in nature in sufficient amounts to allow their commercial exploitation. Consequently, so-called rare sugars have to be produced by (bio)chemical processes starting from cheap and widely available substrates. Three enzyme classes that can be used for rare sugar production are keto-aldol isomerases, epimerases, and oxidoreductases. In this review, the recent developments in rare sugar production with these biocatalysts are discussed.

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Biosynthesis of rare ketoses through constructing a recombination pathway in an engineered Corynebacterium glutamicum

Yang

Zhu

et al. 2014

Biotech & Bioengineering

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Rare sugars have various known biological functions and potential for applications in pharmaceutical, cosmetics, and food industries. Here we designed and constructed a recombination pathway in Corynebacterium glutamicum, in which dihydroxyacetone phosphate (DHAP), an intermediate of the glycolytic pathway, and a variety of aldehydes were condensed to synthesize rare ketoses sequentially by rhamnulose-1-phosphate aldolase (RhaD) and fructose-1-phosphatase (YqaB) obtained from Escherichia coli. A wild-type strain harboring this artificial pathway had the ability to produce D-sorbose and D-psicose using D-glyceraldehyde and glucose as the substrates. The tpi gene, encoding triose phosphate isomerase was further deleted, and the concentration of DHAP increased to nearly 20-fold relative to that of the wild-type. After additional optimization of expression levels from rhaD and yqaB genes and of the fermentation conditions, the engineered strain SY6(pVRTY) exhibited preferable performance for rare ketoses production. Its yield increased to 0.59 mol/mol D-glyceraldehyde from 0.33 mol/mol D-glyceraldehyde and productivity to 2.35 g/L h from 0.58 g/L h. Moreover, this strain accumulated 19.5 g/L of D-sorbose and 13.4 g/L of D-psicose using a fed-batch culture mode under the optimal conditions. In addition, it was verified that the strain SY6(pVRTY) meanwhile had the ability to synthesize C4, C5, C6, and C7 rare ketoses when a range of representative achiral and homochiral aldehydes were applied as the substrates. Therefore, the platform strain exhibited the potential for microbial production of rare ketoses and deoxysugars.

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Cloning and Overexpression of the Xylitol Dehydrogenase Gene from Bacillus pallidus and Its Application to L-Xylulose Production

Cited by 20 publications

References 32 publications

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Repeated production of l-xylulose by an immobilized whole-cell biocatalyst harboring l-arabinitol dehydrogenase coupled with an NAD+ regeneration system

Enzymes for the biocatalytic production of rare sugars

Biosynthesis of rare ketoses through constructing a recombination pathway in an engineered Corynebacterium glutamicum

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