Cloning and nucleotide sequence of a cDNA encoding the mink growth hormone

Detection, Analysis and Interactions of Plasma Ghrelin, Leptin and Growth Hormone in the Mink (Mustela vison)

“…The plasma leptin levels were determined with the Multi-Species Leptin RIA kit (Linco Research, St Charles, MO, USA; 2.8-3.6 and 6.5-8.7% CV) and the plasma GH levels using the hGH Double Antibody Human Growth Hormone kit (Diagnostic Products Corporation, Los Angeles, CA, USA; 1.5-5.9 and 1.8-8.3% CV). The mink GH shares a 66% amino acid homology with the human GH (Shoji et al , 1990), whereas the homologies between the human and mink leptin and ghrelin molecules are unknown. The plasma ghrelin, leptin and GH assays were validated such that serial dilutions of the mink plasma showed linear changes in B/B 0 values that were parallel with the standard curves produced with human standards (Fig.…”

Growth hormone and growth hormone gene of the American mink (Neovison vison) – the current state of knowledge of one of the key hormones in one of the most intensively economically exploited species

“…The mGH particle (accession code UNIPROT: P19795 SOMA_MUSVI) has the structure of a simple polypeptide chain, composed of 190 amino acids (in the largest amount there is leucine, consisting 13.2% of all amino acids, while in the smallest one -tryptophan, consisting 0,5%), with the molecular weight of 21,717.84 Da (Harada et al, 1990;Rice et al, 2000). The mature hormone is formed from the precursor molecule, consisting of 216 amino acids, of which 26 form a signal peptide proteolytically cleaved during the post-translational processing (Shoji et al, 1990). The molecular formula of mGH is C 973 H 1519 N 263 O 285 S 8 , and the theoretical value of the isoelectric point (pI) is 7.38 (Rice et al, 2000;Artimo et al, 2012).…”

“…The growth hormone of American mink, like in case of other mammals, contains in its structure of the polypeptide chain two internal disulfide bonds (disulfide bridges) between cysteines in the position of Cys78-Cys189 and Cys206-Cys214 (Watahiki et al, 1989;Shoji et al, 1990). Also the presence of two zinc binding sites in the amino acid sequence of the GH polypeptide of N. vison was shown -in the position of His45 and Glu198 (The UniProt Consortium, 2012).…”

“…cDNA for the GH gene of American mink (mGH) was sequenced and described for the first time by Shoji et al (1990) (GenBank: X56120.1). The sequence deposited in GenBank was then verified and supplemented by Harada et al (1990) (GenBank: E04303.1) and Perelygina et al (1991) (GenBank: E04303.1).…”

“…The sequence deposited in GenBank was then verified and supplemented by Harada et al (1990) (GenBank: E04303.1) and Perelygina et al (1991) (GenBank: E04303.1). The total length coding sequence (CDS) was specified on 648 bp (Shoji et al, 1990). There is also known the sequence of untranslated regios (UTR) in position 5' (fragment) and 3', enabling the localisation of the poliadenylation signal (5'-AATAAA-3') in position c.*84 -*89, as well as the flanking region 3' (Harada et al, 1990;Shoji et al, 1990).…”