Cloning and molecular characterization of an oral papillomavirus of domestic rabbits

O’Banion, M. Kerry; Me, Cialkowski; Me, Reichmann; Jp, Sundberg

doi:10.1016/0042-6822(88)90411-4

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…The seed stock of infectious ROPV was obtained from papillomas found on the undersurfaces of the tongues of several domestic New Zealand White rabbits. PCR amplification techniques indirectly detected ROPV DNA with primers designed from previously published partial ROPV sequence data (14). We have sequenced the entire genome of a cloned isolate of this virus, and the data confirm that the virus is ROPV (data not shown).…”

mentioning

confidence: 52%

Rabbit Genital Tissue Is Susceptible to Infection by Rabbit Oral Papillomavirus: an Animal Model for a Genital Tissue-Targeting Papillomavirus

Sb¹,

Nm²,

Lr³

et al. 1998

J Virol

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Rabbit oral papillomavirus (ROPV) is a mucosatropic papillomavirus which naturally infects oral mucosal sites of domestic rabbits. In this study, we tested the hypothesis that rabbit genital mucosa is also susceptible to ROPV infection by using the athymic mouse xenograft system and adult immunocompetent rabbits. Subrenal xenografts of ROPV-infected rabbit vulvar and penile sheath tissues were strongly positive for ROPV infection by histologic, in situ hybridization, and Southern analyses. Direct inoculation of adult rabbit penises with infectious ROPV produced small raised lesions of approximately 1 by 1 by 1 mm that were ROPV positive by both in situ hybridization and Southern analyses and were also viral capsid antigen positive by immunohistological staining. Infection of rabbit genital tissues with ROPV may be a useful animal model for the study of genital tissue-targeting papillomaviruses.

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mentioning

confidence: 52%

Rabbit Genital Tissue Is Susceptible to Infection by Rabbit Oral Papillomavirus: an Animal Model for a Genital Tissue-Targeting Papillomavirus

Sb¹,

Nm²,

Lr³

et al. 1998

J Virol

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“…A papillomavirus (MmPV) from the European harvest mouse Min"omys minutus has been cloned and characterized (O'Banion et al, 1988a). This virus has been associated with a variety of tumours including papillomas and sebaceous carcinomas and has homology with rabbit oral papillomavirus (ROPV), MnPV and HPV-1a (O'Banion et al, 1988b). Horizontal transmission was successful, although it was not possible to transmit the virus to inbred strains of laboratory mice .…”

Section: Rodent Modelsmentioning

confidence: 95%

“…A further virus, ROPV, infects domestic rabbits causing oral papillomas (not transmissible to the genital tract), with abundant virion production (Sundberg et al, 1985). The genome has been cloned and partially sequenced (O'Banion et al, 1988b), showing similarities with CRPV, HPV-la, HPV-16 and BPV-5. Rabbit tongue tissues have been incubated with ROPV and placed subrenally into athymic mice, producing stocks of infectious ROPV particles (Christensen et al, 1996).…”

Section: Transgenic Rabbit Modelmentioning

confidence: 99%

In vitroand Animal Models for Antiviral Therapy in Papillomavirus Infections

Stanley

Masterson

Nicholls

1997

Antivir Chem Chemother

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SummaryThe need for antiviral therapies for papillomavirus infections is well recognized but the difficulties of reproducing the infectious cycle of papillomaviruses in vitro has hindered our understanding of virus-cell interactions and the regulation of viral gene expression during permissive growth. Recent advances in understanding the temporal expression and function of papillomavirus proteins. has enabled consideration of a targeted approach to papillomavirus chemotherapy and in particular the inhibition of viral replication by targeting the El and E2 proteins. There are in vitro culture systems available for the screening of new chemotherapeutic agents, since significant advances have been made with culture systems which promote epithelial differentiation in vitro. However, to date, there are no published data which show that virions generated in vitro can infect keratinocytes and initiate another round of replication in vitro. In vivo animal models are therefore necessary to assess the efficacy of antivirals in preventing and treating viral infection, particularly for the low-risk genital viruses which are on the whole refractory to culture in vitro. Although papillomaviruses affect a wide variety of hosts in a species-specific manner, the animals most useful for modelling papillomavirus infections include the rabbit, ox, mouse, dog, horse, primate and sheep. The ideal animal model should be widely available, easy to house and handle, be large enough to allow for adequate tissue sampling, develop lesions on anatomical sites comparable with those in human diseases and these lesions should be readily accessible for monitoring and ideally should yield large amounts of infectious virus particles for use in both in vivo and in vitro studies. The relative merits of the various papillomavirus animal models available in relation to these criteria are discussed.

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“…Since this time, there have been very few published reports describing this PV system (Richter et al, 1964;Rdzok et al, 1966;Weisbroth & Scher, 1970;Dominguez et al, 1981 ;Sundberg et al, 1985;O'Banion eta]., 1988), and only limited stocks of infectious virus have been reported. A more recent study has described the cloning and partial sequencing of the ROPV genome (O'Banion et al, 1988), and the published sequence data were used to help confirm indirectly that the PV isolate described in this study was ROPV. In an earlier study, cloning of the ROPV genome was achieved as three separate subgenomic fragments (O'Banion etal., 1988) because a restriction enzyme that cut the ROPV genome once was not found.…”

Section: Discussionmentioning

confidence: 99%

“…Rabbit oral papillomavirus (ROPV) is a mucosal-targeting papillomavirus (PV) that causes benign lesions on the tongue and in the oral cavity of domestic rabbits (Parsons & Kidd, 1936, 1942Richter et al, 1964;Rdzok et al, 1966;Weisbroth & Scher, 1970;Dominguez et al, 1981;Sundberg et al, 1985;O'Banion et al, 1988). ROPV, in contrast to cottontail rabbit papillomavirus (CRPV) (a cutaneous PV associated with squamous cell carcinomas), does not infect cutaneous sites, is antigenically unrelated to CRPV, and induces lesions that often regress spontaneously (Parsons & Kidd, 1942;Rdzok et al, 1966).…”

Section: Introductionmentioning

confidence: 99%