Cloning and molecular characterization of the glucose transporter 1 in tilapia (Oreochromis niloticus)

Hrytsenko, Olga; Pohajdak, Bill; Xu, Bing; Morrison, Carol M.; vanTol, Brenna; Wright, James R.

doi:10.1016/j.ygcen.2009.07.017

Cited by 30 publications

(17 citation statements)

References 60 publications

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“…29 Cloning of the partial sequence of the NTins2 gene Based on the alignment of the available sequences for fish insulin 2 genes, we designed degenerative primers: In2-S1, In2-AS1, In2-S2, In2-AS2, and used them together with the tilapia genomic DNA in 2 consecutive rounds of PCR. The first round was performed with In2-S1and In2-AS1 primers, followed by the second round with In2-S2 and In2-AS2 primers.…”

Section: Fish Maintenance and Tissue Isolationmentioning

confidence: 99%

Ancestral genomic duplication of the insulin gene in tilapia: An analysis of possible implications for clinical islet xenotransplantation using donor islets from transgenic tilapia expressing a humanized insulin gene

Pohajdak

Wright

2016

Islets

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Tilapia, a teleost fish, have multiple large anatomically discrete islets which are easy to harvest, and when transplanted into diabetic murine recipients, provide normoglycemia and mammalian-like glucose tolerance profiles. Tilapia insulin differs structurally from human insulin which could preclude their use as islet donors for xenotransplantation. Therefore, we produced transgenic tilapia with islets expressing a humanized insulin gene. It is now known that fish genomes may possess an ancestral duplication and so tilapia may have a second insulin gene. Therefore, we cloned, sequenced, and characterized the tilapia insulin 2 transcript and found that its expression is negligible in islets, is not islet-specific, and would not likely need to be silenced in our transgenic fish.

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Section: Fish Maintenance and Tissue Isolationmentioning

confidence: 99%

Ancestral genomic duplication of the insulin gene in tilapia: An analysis of possible implications for clinical islet xenotransplantation using donor islets from transgenic tilapia expressing a humanized insulin gene

Pohajdak

Wright

2016

Islets

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“…It is known that glucose is a critical nutrient for the brain and that transport of this hexose from blood to brain is mediated by the blood-brain barrier (BBB) GLUT1 glucose transporter in mammals. Expression of the GLUT1 transporter has been also described in the brain of several fish species, including rainbow trout (Teerijoki et al, 2000;Hall et al, 2005;Hrytsenko et al, 2010). In rats, increased expression of GLUT1 mRNA at the BBB has been demonstrated in chronic hypoglycemia (Boado and Pardridge, 1993;Kumagai et al, 1995) and it has also been shown that glucose infusion after a fasting period induces acute hyperglycemia in the brain (Béquet et al, 2000).…”

Section: Brain Glycogen Supercompensation After Fasting-induced Hypogmentioning

confidence: 97%

Brain glycogen supercompensation after different conditions of induced hypoglycemia and sustained swimming in rainbow trout ( Oncorhynchus mykiss )

Blanco

Gómez-Boronat

Maceira

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

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“…GLUT 1 protein has been detected in tissues of teleost fish (Wright et al 2000;Moon 2001). In Nile tilapia, it has been observed that GLUT 1 is widely distributed in various tissues (Hrytsenko et al 2010); it is strongly expressed in erythrocytes and brain whereas liver, heart, and muscle all have exhibited low levels of expression (Wright et al 1998;Klepper et al 1999). GLUT 1 is a stress-inducible protein of the glucose-regulated proteins superfamily (Wertheimer et al 1991) of which the expression level is modulated by stress (Hwang and Ismail-Beigi 2001).…”

Section: Introductionmentioning

confidence: 99%

“…For instance, Wright et al (1998) reported the presence of GLUT 1 protein in heart and brain of tilapia; and increased glucose uptake and metabolism in transgenic trout embryos overexpressing human GLUT 1. Hrytsenko et al (2010) analyzed the effect of acute glucose injection and feeding on the level of GLUT 1 mRNA expression in Nile tilapia, and found that both food and glucose upregulated GLUT 1 mRNA levels in white muscle. However, little is known about how chronic stress affects levels of GLUT 1 in Nile tilapia.…”

Section: Introductionmentioning

confidence: 99%

Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

et al. 2014

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Fish farmers have a tendency of employing high stocking density (HSD) as a means of increasing productivity. However, HSD is a chronic stressor that is likely to lower profitability of fish farming if not implemented properly. HSD induces stress which in turn elevates sequentially the levels of plasma cortisol and glucose. The resultant glucose is distributed to various tissues by glucose transporter protein (GLUTs) to restore normalcy. GLUT 1, transmembrane protein found in erythrocytes, is responsible for import and export of glucose in red blood cells. However, knowledge on how chronic stress impact glucose and GLUT 1 protein in fish subjected to HSD is still unclear. In this study, effect of HSD on the expression of GLUT 1 in Nile tilapia was investigated in an attempt to elucidate the role of GLUT 1 in glucose metabolism during chronic stress. Fish were reared for 4 weeks at 1.5 and 4.5 kg/m 3 for low stocking density (LSD) and HSD, respectively. Four physiological parameters were determined from the blood samples obtained from fish at the end of experiment. At p \ 0.05, there were significant differences between fish reared at HSD and LSD in plasma cortisol level (72.1 ± 5.9 ng/ml and 37.5 ± 4.6 ng/ml); blood glucose level (136.00 ± 1.3 mg/dL and 70.2 ± 1.0 mg/dL); erythrocytes count (7.2 ± 0.5 9 106 mm -3 and 2.1 ± 0.4 9 106 mm -3 ); and plasma GLUT 1 level (1.40 ± 0.17 rbi and 0.81 ± 0.07 rbi), respectively. HSD induced elevation of plasma cortisol level, blood glucose level, erythrocytes count and GLUT 1 level. These elevated physiological factors and particularly GLUT 1 can be used as a cellular stress biomarker in fish farming and aquaculture.

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Cloning and molecular characterization of the glucose transporter 1 in tilapia (Oreochromis niloticus)

Cited by 30 publications

References 60 publications

Ancestral genomic duplication of the insulin gene in tilapia: An analysis of possible implications for clinical islet xenotransplantation using donor islets from transgenic tilapia expressing a humanized insulin gene

Ancestral genomic duplication of the insulin gene in tilapia: An analysis of possible implications for clinical islet xenotransplantation using donor islets from transgenic tilapia expressing a humanized insulin gene

Brain glycogen supercompensation after different conditions of induced hypoglycemia and sustained swimming in rainbow trout ( Oncorhynchus mykiss )

Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

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