Cloning and molecular characterization of a yak α-lactalbumin cDNA from mammary tissue

Bai, Wen; Yin, Rong; Zheng, Yusheng; Ma, Zhenghua; Zhong, Jincheng; Yin, Renfu; Dou, Q.L.; Zhang, S.C.; Luo, G.B.; Zhao, Zhenghua

doi:10.1016/j.livsci.2010.01.012

Cited by 6 publications

(3 citation statements)

References 25 publications

(28 reference statements)

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“…The yak has high similarities with domestic cattle ( Bos taurus ) in amino acid sequence of proteins assayed [10,11]. Thus, yak would be a suitable model to study the molecular adaptation to hypoxia in bovine species.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

Wang

Zhao

Zhong

et al. 2013

IJMS

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The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.

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Section: Discussionmentioning

confidence: 99%

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

Wang

Zhao

Zhong

et al. 2013

IJMS

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“…Because of the high sequence similarity (98 to 99%) between yak and Yellow cattle in genes assayed (Zheng et al, 2008;Bai et al, 2010;Zhang et al, 2010), yak may act as a suitable animal model to study the mechanism of hypoxia adaptation. Due to the low oxygen environment, energy supply is an important aspect for studying adaptation in yaks.…”

Section: Discussionmentioning

confidence: 99%

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles

Lin¹,

Xu²,

Yu³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Hypoxia can affect energy metabolism. We examined gene expression and enzyme activity related to mitochondrial energy metabolism, as well as myosin heavy chain (MyHC) types in yaks (Bos grunniens) living at high altitudes. Real-time quantitative PCR assays indicated that the yak has significantly lower levels of carnitine palmitoyltransferase (CPT) mRNA in the biceps femoris and lower levels of uncoupling protein 3 (UCP3) mRNA in both biceps femoris and longissimus dorsi than in Yellow cattle. No significant differences between yak and Yellow cattle were observed in the activities of mitochondrial β-hydroxyacyl-CoA dehydrogenase, isocitrate dehydrogenase and cytochrome oxidase in the same muscles. Semiquantitative RT-PCR analysis showed that the MyHC 1 mRNA levels in yak biceps femoris was lower than in Yellow cattle. We conclude that the yak has significantly lower mRNA levels of CPT, UCP3, and MyHC 1 in biceps femoris than in Yellow cattle, suggesting that the yak biceps femoris has lower fatty acid oxidation capacity and greater glycolytic metabolic potential.

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“…Many candidate genes have been presented for muscle growth and meat quality in cattle and other domestic animals (de Koning et al, 1999;Damon et al, 2006;Hoashi et al, 2008), but they are nearly unavailable in yaks. The yak shows great similarities of amino acid sequences with domestic cattle (Bos taurus) in proteins assayed (Zheng et al, 2008;Bai et al, 2010;Zhang et al, 2010). Thus, the yak would be a suitable model to study the influences of genetic or environmental factors on performances in bovine species.…”

mentioning

confidence: 99%

Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle

Zheng¹,

Lin²,

Yu³

et al. 2011

CZECH J ANIM SCI

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ABSTRACT:The main objective of this study was to reveal the expression profiles of two negative regulators, myostatin (MSTN) and calpastatin (CAST) genes, of skeletal muscle growth in highland yaks (Bos grunniens). mRNA levels of both genes were quantified in different yak tissues by semi-quantitative RT-PCR to reveal the tissue expression pattern, and real-time quantitative RT-PCR was employed to compare the mRNA levels of MSTN and CAST in longissimus muscles of yaks at different ages and adult Yellow cattle. Intramuscular fat (IMF) content, tenderness and pH of longissimus muscle of yaks at different ages and of adult Yellow cattle were also measured. The results showed that MSTN and CAST expressions have tissue specificity and both exhibited a high level in longissimus muscle and a low level in adipose tissue. Yak calves had lower mRNA levels of both MSTN and CAST in longissimus muscle compared with adult yaks. The analysis of meat quality traits of longissimus muscle showed that the shear forces of raw longissimus muscle of yak calves were significantly lower than those of adult yaks and Yellow cattle, no significant difference was found between adult yaks and Yellow cattle of similar age. IMF content in longissimus muscle was lower in yaks than in Yellow cattle. Although yaks were smaller in body size than Yellow cattle, adult yaks showed lower levels of MSTN and similar level of CAST mRNA in longissimus muscle compared to Yellow cattle. These data indicate that the expression of both MSTN and CAST in longissimus muscle differs between adult yaks and yak calves, and the yak longissimus muscle shows a lower IMF content compared to cattle.

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Cloning and molecular characterization of a yak α-lactalbumin cDNA from mammary tissue

Cited by 6 publications

References 25 publications

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene

Differences in mitochondrial gene expression profiles, enzyme activities and myosin heavy chain types in yak versus bovine skeletal muscles

Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle

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