Cloning and Molecular Characterization of Hev b 3, a Spina–bifida–Associated Allergen from &lt;i&gt;Hevea Brasiliensis&lt;/i&gt; Latex

Scheiner, Otto; Wagner, B.J.; Wagner, Stefan; Krebitz, Monika; Crameri, Reto; Niggemann, B.; Yeang, Hoong Yeet; Ebner, Christoph; Breiteneder, Heimo

doi:10.1159/000024113

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…With the exception of two sera, all Hev b 3-positive individuals produced sIgE reactive with rHev b 1. These data support the observations of Wagner et al [25], that the insoluble particle-bound allergens Hev b 1 and 3 contain similar IgE epitopes and that the suspected route of sensitization (mucosal) is the same for both. Based on this insolubility, both allergens are much harder to aerosolize, and their sensitization process thus seems to require direct contact of latex with blood or mucosal surfaces, which occurs during surgery.…”

Section: Discussionsupporting

confidence: 91%

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Raulf‐Heimsoth

Rihs

Rozynek

et al. 2007

Clin Experimental Allergy

108

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SummaryBackground Characterized native and recombinant Hevea brasiliensis (rHev b) natural rubber latex (NRL) allergens are available to assess patient allergen sensitization profiles.Objective Quantification of individual IgE responses to the spectrum of documented NRL allergens and evaluation of cross-reactive carbohydrate determinants (CCDs) for more definitive diagnosis. Methods Sera of 104 healthcare workers (HCW; 51 German, 21 Portuguese, 32 American), 31 spina bifida patients (SB; 11 German, 20 Portuguese) and 10 Portuguese with multiple surgeries (MS) were analysed for allergen-specific IgE antibody (sIgE) to NRL, single Hev b allergens and CCDs with ImmunoCAP TM technology. Results In all patient groups rHev b 5-sIgE concentrations were the most pronounced. Hev b 2, 5, 6.01 and 13 were identified as the major allergens in HCW and combined with Hev b 1 and Hev b 3 in SB. In MS Hev b 1 displayed an intermediate relevance. Different sIgE antibody levels to native Hevea brasiliensis (nHev b) 2 and rHev b 6.01 allowed discrimination of SB with clinical relevant latex allergy vs. those with latex sensitization. Sensitization profiles of German, Portuguese and American patients were equivalent. rHev b 5, 6.01 and nHev b 13 combined detected 100% of the latex-allergic HCW and 80.1% of the SB. Only 8.3% of the sera showed sIgE response to CCDs. Conclusions Hev b 1, 2, 5, 6.01 and 13 were identified as the major Hev b allergens and they should be present in standardized latex extracts and in vitro allergosorbents. CCDs are only of minor relevance in patients with clinical relevant latex allergy. Component-resolved diagnostic analyses for latex allergy set the stage for an allergen-directed immunotherapy strategy.

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Section: Discussionsupporting

confidence: 91%

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Raulf‐Heimsoth

Rihs

Rozynek

et al. 2007

Clin Experimental Allergy

108

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“…The full alignment of the REF/SRPP proteins from H. brasiliensis is showed in Supplementary Figure 1, and the homology of both REF (P15252) and SRPP (O82803) genes we used in this study is presented in Figure 2A with the sequence consensus of the hevea family. This important homology (>40%) was previously described [8], [13], [29], [37] and their possible role during stress was previously proposed as their mRNA expression increases upon tapping [32], [33].…”

Section: Resultssupporting

confidence: 70%

Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties

et al. 2012

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REF (Hevb1) and SRPP (Hevb3) are two major components of Hevea brasiliensis latex, well known for their allergenic properties. They are obviously taking part in the biosynthesis of natural rubber, but their exact function is still unclear. They could be involved in defense/stress mechanisms after tapping or directly acting on the isoprenoid biosynthetic pathway. The structure of these two proteins is still not described. In this work, it was discovered that REF has amyloid properties, contrary to SRPP. We investigated their structure by CD, TEM, ATR-FTIR and WAXS and neatly showed the presence of β-sheet organized aggregates for REF, whereas SRPP mainly fold as a helical protein. Both proteins are highly hydrophobic but differ in their interaction with lipid monolayers used to mimic the monomembrane surrounding the rubber particles. Ellipsometry experiments showed that REF seems to penetrate deeply into the monolayer and SRPP only binds to the lipid surface. These results could therefore clarify the role of these two paralogous proteins in latex production, either in the coagulation of natural rubber or in stress-related responses. To our knowledge, this is the first report of an amyloid formed from a plant protein. This suggests also the presence of functional amyloid in the plant kingdom.

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“…Certain allergens can be attributed to different clinical entities. While spina bifida is associated with Hev b 1, Hev b 3 (35)(36)(37), and Hev b 7 (38) in health care workers, problems are associated with Hev b 1 (39), Hev b 6, and in the latex-fruit syndrome with Hev b 2 and Hev b 8 (40,41).…”

Section: Allergensmentioning

confidence: 99%

IgE-mediated latex allergy - An exciting and instructive piece of allergy history

Niggemann

2010

Pediatric Allergy and Immunology

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Natural rubber latex represents a potent allergen, which for many years had an important impact on occupational health problems but especially on certain risk groups such as spina bifida. Luckily, these problems decreased when powder-free, latex-poor gloves were introduced. Latest data show that in children with spina bifida, who grew up completely latex-free from birth on, sensitization to NRL as well as clinical relevant allergy significantly decreased. Furthermore, sensitization to aeroallergens also went down and even the prevalence of allergic diseases decreased significantly to rates of the general population. This new data clearly indicates that potent allergens (such as latex) in high-risk groups (such as spina bifida) can induce sensitization spreading, and corresponding avoidance can reverse this development. In conclusion, it can be stated that 'new' allergies can suddenly arise, there are allergen-specific risk groups, local IgE-production is also possible in the CNS, allergen avoidance can be very effective in terms of primary prevention, sensitization spreading can be made reversible by effective prevention, and finally, certain allergies can luckily become history within a relatively short period of time. Furthermore, these new findings clearly end the debate about whether patients with spina bifida have a disease-inherited risk for allergy to NRL and show that the cause is the meningi and the multiple surgical interventions - and therefore sequelae can be reversed by implementing preventive measures.

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Cloning and Molecular Characterization of Hev b 3, a Spina–bifida–Associated Allergen from <i>Hevea Brasiliensis</i> Latex

Cited by 9 publications

References 13 publications

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Quantitative analysis of immunoglobulin E reactivity profiles in patients allergic or sensitized to natural rubber latex (Hevea brasiliensis)

Rubber Elongation Factor (REF), a Major Allergen Component in Hevea brasiliensis Latex Has Amyloid Properties

IgE-mediated latex allergy - An exciting and instructive piece of allergy history

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