Cloning and molecular characterization of an ornithine decarboxylase gene and its expression during embryonic development of the housefly, Musca domestica

Abstract

We are interested in identifying targets that may be used to develop new control products for the common housefly, Musca domestica, a vector of disease for many vertebrates. One such target, ornithine decarboxylase (ODC), is an embryonic enzyme involved in the regulation of polyamines and is a critical enzyme during M. domestica development. In this study, the cDNA for ODC from M. domestica was cloned, sequenced, and characterized. The full-length cDNA was 1,337-bp, consistent with a single band of approximately 1.35 kb obtained by northern analysis. The open-reading frame contains 1,191 bp, yielding a deduced polypeptide of 396 amino acid residues with a predicted mass of 44,618 Da. The deduced M. domestica ODC protein was homologous to other ODC proteins. mRNA expression profiles analyzed by real-time PCR indicated that the ODC transcript is temporally regulated throughout embryogenesis. Sequence data and Southern blot analysis suggests that there were likely only one or two closely linked copies of the M. domestica ODC gene.

SEQUENCING AND CHARACTERIZATION OF GLYCOGEN SYNTHASE AND GLYCOGEN PHOSPHORYLASE GENES FROM Spodoptera exigua AND ANALYSIS OF THEIR FUNCTION IN STARVATION AND EXCESSIVE SUGAR INTAKE

“…After hybridization, the membrane was washed with 0.2 × SSPE (0.03 M NaCl, 0.002 M NaH 2 PO 4 , 0.0002. M EDTA, pH 7.4) at 45°C and exposed to X‐ray film at −70°C for 24 h (Tang et al, ; Rivera‐Vega et al, ; Wang et al, ; Toutges et al, ). rRNA was used as a control.…”